A Novel Thermal Bubble Valve Integrated Nanofluidic Preconcentrator for Highly Sensitive Biomarker Detection

Chih Zong Deng, Yu Jui Fan, Pei Shan Chung, Horn Jiunn Sheen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this study, we developed a new immunosensor that can achieve an ultralow detection limit and high sensitivity. This new device has an electrokinetic trapping (EKT)-based nanofluidic preconcentrator, which was integrated with oscillating bubble valves, to trap concentrated antigen and immunobeads. During the immunoassay process, oscillating bubbles rapidly grew and acted as control valves and to block the microchannel. Thereafter, the trapped preconcentrated antigen plug and antibody-coated nanobeads were preserved in the region between these two valves. Finally, the antigen concentration was quantitatively analyzed by a real-time measurement of Brownian diffusion of the immunobeads. In this work, the test sample used was C-reactive protein (CRP) which is a risk indicator of coronary heart disease and atherosclerosis.

Original languageEnglish
Pages (from-to)1409-1415
Number of pages7
JournalACS Sensors
Volume3
Issue number7
DOIs
Publication statusPublished - Jul 27 2018

Fingerprint

Nanofluidics
biomarkers
Biomarkers
antigens
Antigens
bubbles
control valves
heart diseases
arteriosclerosis
Immunosensors
immunoassay
electrokinetics
plugs
microchannels
Microchannels
Time measurement
antibodies
Antibodies
C-Reactive Protein
trapping

Keywords

  • acoustic wave
  • Brownian motion
  • C-reactive protein
  • immunobeads
  • nanofluidic preconcentrator
  • oscillating thermal microbubble

ASJC Scopus subject areas

  • Bioengineering
  • Instrumentation
  • Process Chemistry and Technology
  • Fluid Flow and Transfer Processes

Cite this

A Novel Thermal Bubble Valve Integrated Nanofluidic Preconcentrator for Highly Sensitive Biomarker Detection. / Deng, Chih Zong; Fan, Yu Jui; Chung, Pei Shan; Sheen, Horn Jiunn.

In: ACS Sensors, Vol. 3, No. 7, 27.07.2018, p. 1409-1415.

Research output: Contribution to journalArticle

Deng, Chih Zong ; Fan, Yu Jui ; Chung, Pei Shan ; Sheen, Horn Jiunn. / A Novel Thermal Bubble Valve Integrated Nanofluidic Preconcentrator for Highly Sensitive Biomarker Detection. In: ACS Sensors. 2018 ; Vol. 3, No. 7. pp. 1409-1415.
@article{86b2a189b63c459b8084dd76239b851e,
title = "A Novel Thermal Bubble Valve Integrated Nanofluidic Preconcentrator for Highly Sensitive Biomarker Detection",
abstract = "In this study, we developed a new immunosensor that can achieve an ultralow detection limit and high sensitivity. This new device has an electrokinetic trapping (EKT)-based nanofluidic preconcentrator, which was integrated with oscillating bubble valves, to trap concentrated antigen and immunobeads. During the immunoassay process, oscillating bubbles rapidly grew and acted as control valves and to block the microchannel. Thereafter, the trapped preconcentrated antigen plug and antibody-coated nanobeads were preserved in the region between these two valves. Finally, the antigen concentration was quantitatively analyzed by a real-time measurement of Brownian diffusion of the immunobeads. In this work, the test sample used was C-reactive protein (CRP) which is a risk indicator of coronary heart disease and atherosclerosis.",
keywords = "acoustic wave, Brownian motion, C-reactive protein, immunobeads, nanofluidic preconcentrator, oscillating thermal microbubble",
author = "Deng, {Chih Zong} and Fan, {Yu Jui} and Chung, {Pei Shan} and Sheen, {Horn Jiunn}",
year = "2018",
month = "7",
day = "27",
doi = "10.1021/acssensors.8b00323",
language = "English",
volume = "3",
pages = "1409--1415",
journal = "ACS Sensors",
issn = "2379-3694",
publisher = "American Chemical Society",
number = "7",

}

TY - JOUR

T1 - A Novel Thermal Bubble Valve Integrated Nanofluidic Preconcentrator for Highly Sensitive Biomarker Detection

AU - Deng, Chih Zong

AU - Fan, Yu Jui

AU - Chung, Pei Shan

AU - Sheen, Horn Jiunn

PY - 2018/7/27

Y1 - 2018/7/27

N2 - In this study, we developed a new immunosensor that can achieve an ultralow detection limit and high sensitivity. This new device has an electrokinetic trapping (EKT)-based nanofluidic preconcentrator, which was integrated with oscillating bubble valves, to trap concentrated antigen and immunobeads. During the immunoassay process, oscillating bubbles rapidly grew and acted as control valves and to block the microchannel. Thereafter, the trapped preconcentrated antigen plug and antibody-coated nanobeads were preserved in the region between these two valves. Finally, the antigen concentration was quantitatively analyzed by a real-time measurement of Brownian diffusion of the immunobeads. In this work, the test sample used was C-reactive protein (CRP) which is a risk indicator of coronary heart disease and atherosclerosis.

AB - In this study, we developed a new immunosensor that can achieve an ultralow detection limit and high sensitivity. This new device has an electrokinetic trapping (EKT)-based nanofluidic preconcentrator, which was integrated with oscillating bubble valves, to trap concentrated antigen and immunobeads. During the immunoassay process, oscillating bubbles rapidly grew and acted as control valves and to block the microchannel. Thereafter, the trapped preconcentrated antigen plug and antibody-coated nanobeads were preserved in the region between these two valves. Finally, the antigen concentration was quantitatively analyzed by a real-time measurement of Brownian diffusion of the immunobeads. In this work, the test sample used was C-reactive protein (CRP) which is a risk indicator of coronary heart disease and atherosclerosis.

KW - acoustic wave

KW - Brownian motion

KW - C-reactive protein

KW - immunobeads

KW - nanofluidic preconcentrator

KW - oscillating thermal microbubble

UR - http://www.scopus.com/inward/record.url?scp=85048512121&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048512121&partnerID=8YFLogxK

U2 - 10.1021/acssensors.8b00323

DO - 10.1021/acssensors.8b00323

M3 - Article

AN - SCOPUS:85048512121

VL - 3

SP - 1409

EP - 1415

JO - ACS Sensors

JF - ACS Sensors

SN - 2379-3694

IS - 7

ER -