A novel synthetic bipartite carrier protein for developing glycotope-based vaccines

Hsiao Ling Chiang, Chi Yu Lin, Fan Dan Jan, Yaoh Shiang Lin, Chia Tse Hsu, Jacqueline Whang-Peng, Leroy F. Liu, Shin Nieh, Chun Cheng Lin, Jaulang Hwang

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Development of successful vaccines against glycotopes remains a major challenge. In the current studies, we have successfully developed a novel carrier protein for glycotopes based on the concept of antigen clustering and specific stimulation of T helper cells to mount strong antibody response to glycotopes. The bipartite carrier protein consists of a tandem repeat of a cysteine-rich peptide for docking of clustered glycotopes to effectively activate B cells and an Fc domain for antigen delivery to antigen presenting cells (APCs). To demonstrate its utility, we conjugated the tumor-specific monosaccharide antigen Tn to this novel carrier protein and successfully developed a Tn vaccine against cancer in animal models. The Tn vaccine effectively elicited high-titer IgG1 antibodies against Tn in immunized mice, and effectively suppressed the development of prostate cancer in Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP) mice. Our results suggest that this novel bipartite carrier protein could be effectively used for developing anti-glycotope vaccines such as the anticancer Tn vaccine.

Original languageEnglish
Pages (from-to)7573-7581
Number of pages9
JournalVaccine
Volume30
Issue number52
DOIs
Publication statusPublished - Dec 14 2012

Keywords

  • Carrier protein
  • Glycotope
  • Tn antigen
  • Tumor immunotherapy
  • Vaccine

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

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