A novel strategy for corneal endothelial reconstruction with a bioengineered cell sheet

Ging Ho Hsiue, Jui Yang Lai, Ko Hua Chen, Wen-Ming Hsu

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Cellular organization of foreign grafts constructed from cultivated cells is critical to successful graft-host integration and tissue repair. This study described a novel human corneal endothelial cell (HCEC) therapeutic method, where cultivated adult HCEC sheet with uniform orientation was prepared and transplanted to a rabbit cornea. Having a correct morphology and intact barriers, the HCEC sheet was made by the temperature-modulated detachment of monolayered HCECs from thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm)-grafted surfaces and was delivered with proper polarity to the corneal posterior surface by a bioadhesive gelatin disc. Results of the in vivo studies, including the follow-up clinical observations and histological examinations, showed the laminated HCEC sheet was successfully integrated into rabbit cornea denuded with endothelial layer after the biodegradation of gelatin carrier. These data indicate the feasibility of the proposed procedure in cell therapy for corneal endothelial cell loss.

Original languageEnglish
Pages (from-to)473-476
Number of pages4
JournalTransplantation
Volume81
Issue number3
DOIs
Publication statusPublished - Feb 1 2006
Externally publishedYes

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Keywords

  • Cell transplantation
  • Gelatin carrier
  • HCEC sheet
  • PNIPAAm-grafted surface
  • Tissue repair

ASJC Scopus subject areas

  • Transplantation
  • Immunology

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