A novel role for the klebsiella pneumoniae sap (sensitivity to antimicrobial peptides) transporter in intestinal cell interactions, innate immune responses, liver abscess, and virulence

Chun Ru Hsu, I. Wei Chang, Pei Fang Hsieh, Tzu Lung Lin, Pei Yin Liu, Chen Hsiu Huang, Kun Tzu Li, Jin Town Wang

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Klebsiella pneumoniae is an important human pathogen causing hospital-acquired and community-acquired infections. Systemic K. pneumoniae infections may be preceded by gastrointestinal colonization, but the basis of this bacterium's interaction with the intestinal epithelium remains unclear. Here, we report that the K. pneumoniae Sap (sensitivity to antimicrobial peptides) transporter contributes to bacterial-host cell interactions and in vivo virulence. Gene deletion showed that sapA is required for the adherence of a K. pneumoniae blood isolate to intestinal epithelial, lung epithelial, urinary bladder epithelial, and liver cells. Te flsapA mutant was defcient for translocation across intestinal epithelial monolayers, macrophage interactions, and induction of proinflammatory cytokines. In a mouse gastrointestinal infection model, flsapA yielded signifcantly decreased bacterial loads in liver, spleen and intestine, reduced liver abscess generation, and decreased mortality. Tese fndings offer new insights into the pathogenic interaction of K. pneumoniae with the host gastrointestinal tract to cause systemic infection.

Original languageEnglish
Pages (from-to)1294-1306
Number of pages13
JournalJournal of Infectious Diseases
Volume219
Issue number8
DOIs
Publication statusPublished - Apr 8 2019

Keywords

  • Intestinal cell
  • Klebsiella pneumoniae
  • Liver abscess
  • Sap transporter
  • Virulence

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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