A novel RING finger protein, Znf179, modulates cell cycle exit and neuronal differentiation of P19 embryonal carcinoma cells

P. C. Pao, N. K. Huang, Y. W. Liu, S. H. Yeh, S. T. Lin, C. P. Hsieh, A. M. Huang, H. S. Huang, J. T. Tseng, W. C. Chang, Y. C. Lee

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Znf179 is a member of the RING finger protein family. During embryogenesis, Znf179 is expressed in a restricted manner in the brain, suggesting a potential role in nervous system development. In this report, we show that the expression of Znf179 is upregulated during P19 cell neuronal differentiation. Inhibition of Znf179 expression by RNA interference significantly attenuated neuronal differentiation of P19 cells and a primary culture of cerebellar granule cells. Using a microarray approach and subsequent functional annotation analysis, we identified differentially expressed genes in Znf179-knockdown cells and found that several genes are involved in development, cellular growth, and cell cycle control. Flow cytometric analyses revealed that the population of G0/G1 cells decreased in Znf179-knockdown cells. In agreement with the flow cytometric data, the number of BrdU-incorporated cells significantly increased in Znf179-knockdown cells. Moreover, in Znf179-knockdown cells, p35, a neuronal-specific Cdk5 activator that is known to activate Cdk5 and may affect the cell cycle, and p27, a cell cycle inhibitor, also decreased. Collectively, these results show that induction of the Znf179 gene may be associated with p35 expression and p27 protein accumulation, which lead to cell cycle arrest in the G0/G1 phase, and is critical for neuronal differentiation of P19 cells.

Original languageEnglish
Pages (from-to)1791-1804
Number of pages14
JournalCell Death and Differentiation
Volume18
Issue number11
DOIs
Publication statusPublished - Nov 2011

Keywords

  • neuronal differentiation
  • P19 cells
  • p27
  • p35
  • Znf179

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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    Pao, P. C., Huang, N. K., Liu, Y. W., Yeh, S. H., Lin, S. T., Hsieh, C. P., Huang, A. M., Huang, H. S., Tseng, J. T., Chang, W. C., & Lee, Y. C. (2011). A novel RING finger protein, Znf179, modulates cell cycle exit and neuronal differentiation of P19 embryonal carcinoma cells. Cell Death and Differentiation, 18(11), 1791-1804. https://doi.org/10.1038/cdd.2011.52