Abstract

Bone regeneration procedures require alternative graft biomaterials to those for autogenous bone. Therefore, we developed a novel porcine graft using particle sizes of 250-500 μm and 500-1000 μm in rabbit calvarial bone defects and compared the graft properties with those of commercial hydroxyapatite (HA)/beta-tricalcium phosphate (β-TCP) over eight weeks. Surgery was performed in 20 adult male New Zealand white rabbits. During a standardized surgical procedure, four calvarial critical-size defects of 5 mm diameter and 3 mm depth were prepared. The defects were filled with HA/β-TCP, 250-500 μm or 500-1000 μm porcine graft, and control defects were not filled. The animals were grouped for sacrifice at 1, 2, 4, and 8 weeks post-surgery. Subsequently, sample blocks were prepared for micro-computed tomography (micro-CT) scanning and histological sectioning. Similar bone formations were observed in all three treatment groups, although the 250-500 μm porcine graft performed slightly better. Rabbit calvarial bone tissue positively responded to porcine grafts and commercial HA/β-TCP, structural analyses showed similar crystallinity and porosity of the porcine and HA/β-TCP grafts, which facilitated bone formation through osteoconduction. These porcine grafts can be considered as graft substitutes, although further development is required for clinical applications.

Original languageEnglish
Pages (from-to)2523-2536
Number of pages14
JournalMaterials
Volume8
Issue number5
DOIs
Publication statusPublished - 2015

Fingerprint

Grafts
Bone
Defects
Hydroxyapatite
Durapatite
Surgery
Biocompatible Materials
Biomaterials
Tomography
Animals
Phosphates
Porosity
Particle size
Tissue
Scanning

Keywords

  • Bone graft
  • Bone regeneration
  • Hydroxyapatite
  • In vivo test
  • Porcine tissue

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

A novel porcine graft for regeneration of bone defects. / Salamanca, Eisner; Lee, Wei Fang; Lin, Chin Yi; Huang, Haw Ming; Lin, Che Tong; Feng, Sheng-Wei; Chang, Wei Jen.

In: Materials, Vol. 8, No. 5, 2015, p. 2523-2536.

Research output: Contribution to journalArticle

Salamanca, Eisner ; Lee, Wei Fang ; Lin, Chin Yi ; Huang, Haw Ming ; Lin, Che Tong ; Feng, Sheng-Wei ; Chang, Wei Jen. / A novel porcine graft for regeneration of bone defects. In: Materials. 2015 ; Vol. 8, No. 5. pp. 2523-2536.
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