Abstract

An in situ forming gel based on simply blending carboxymethyl hexanoyl chitosan (CHC) with low molecular weight hyaluronic acid (LMW HA) was developed, without needing cross-linking, photopolymerization or thermal treatments. The CHC/LMW HA blends formed nanoparticles and then rapidly transformed into supermolecular hydrogels under stirring. The gel formation mechanism was examined by Förster resonance energy transfer (FRET). The gels were injectable, cytocompatible and biodegradable, and showed shape-persistent behavior and adhesive property. Berberine, an anti-apoptotic and anti-arthritis naturally occurring compound, was encapsulated within the CHC/LMW HA gels. The gels demonstrated a pH-responsive characteristic which were able to release berberine in a sustained manner at pH 6.0 (simulating inflamed arthritic articular cartilage) and the degradation rates were accelerated at pH 7.4 (simulating healed normal tissue). The berberine-loaded gels effectively protected chondrocytes against sodium nitroprusside-induced apoptosis. The gels may be potentially useful as an injectable system for intra-articular drug delivery and cartilage tissue engineering.

Original languageEnglish
Pages (from-to)664-673
Number of pages10
JournalCarbohydrate Polymers
Volume206
DOIs
Publication statusPublished - Feb 15 2019

Fingerprint

Berberine
Hyaluronic acid
Hyaluronic Acid
Chitosan
Polymers
Gels
Molecular weight
Cartilage
Hydrogels
Photopolymerization
Nitroprusside
Cell death
carboxymethyl-hexanoyl chitosan
Drug delivery
Tissue engineering
Energy transfer
Adhesives
Heat treatment
Sodium
Tissue

Keywords

  • Berberine
  • Chitosan
  • Drug delivery
  • Hyaluronic acid
  • Injectable hydrogels

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

Cite this

A novel injectable in situ forming gel based on carboxymethyl hexanoyl chitosan/hyaluronic acid polymer blending for sustained release of berberine. / Lu, Kun Ying; Lin, Yen Chen; Lu, Hsien Tsung; Ho, Yi Cheng; Weng, Shih Chun; Tsai, Min Lang; Mi, Fwu Long.

In: Carbohydrate Polymers, Vol. 206, 15.02.2019, p. 664-673.

Research output: Contribution to journalArticle

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AU - Tsai, Min Lang

AU - Mi, Fwu Long

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