A novel in vitro retinal differentiation model by co-culturing adult human bone marrow stem cells with retinal pigmented epithelium cells

Shih Hwa Chiou, Chung Lan Kao, Chi Hsien Peng, Shih Jen Chen, Yih Wen Tarng, Hung Hai Ku, Yu Chih Chen, Yi Ming Shyr, Ren Shyan Liu, Chien Jen Hsu, De Ming Yang, Wen-Ming Hsu, Cheng Deng Kuo, Chen Hsen Lee

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Human retinal pigment epithelium (HRPE) cells are important in maintaining the normal physiology within the neurosensory retina and photoreceptors. Recently, transplantation of HRPE has become a possible therapeutic approach for retinal degeneration. By negative immunoselection (CD45 and glycophorin A), in this study, we have isolated and cultivated adult human bone marrow stem cells (BMSCs) with multilineage differentiation potential. After a 2- to 4-week culture under chondrogenic, osteogenic, adipogenic, and hepatogenic induction medium, these BMSCs were found to differentiate into cartilage, bone, adipocyte, and hepatocyte-like cells, respectively. We also showed that these BMSCs could differentiate into neural precursor cells (nestin-positive) and mature neurons (MAP-2 and Tuj1-positive) following treatment of neural selection and induction medium for 1 month. Furthermore, the plasticity of BMSCs was confirmed by initiating their differentiation into retinal cells and photoreceptor lineages by co-culturing with HRPE cells. The latter system provides an ex vivo expansion model of culturing photoreceptors for the treatment of retinal degeneration diseases.

Original languageEnglish
Pages (from-to)578-585
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume326
Issue number3
DOIs
Publication statusPublished - Jan 21 2005
Externally publishedYes

Fingerprint

Stem cells
Bone Marrow Cells
Retinal Pigment Epithelium
Bone
Stem Cells
Epithelium
Retinal Pigments
Retinal Degeneration
Glycophorin
Nestin
Retinal Diseases
Cell Lineage
Adipocytes
Cartilage
Physiology
Retina
Hepatocytes
Therapeutics
Transplantation
Neurons

Keywords

  • Human bone marrow stem cell
  • Human retinal pigment epithelium
  • Photoreceptor
  • Retinal differentiation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

A novel in vitro retinal differentiation model by co-culturing adult human bone marrow stem cells with retinal pigmented epithelium cells. / Chiou, Shih Hwa; Kao, Chung Lan; Peng, Chi Hsien; Chen, Shih Jen; Tarng, Yih Wen; Ku, Hung Hai; Chen, Yu Chih; Shyr, Yi Ming; Liu, Ren Shyan; Hsu, Chien Jen; Yang, De Ming; Hsu, Wen-Ming; Kuo, Cheng Deng; Lee, Chen Hsen.

In: Biochemical and Biophysical Research Communications, Vol. 326, No. 3, 21.01.2005, p. 578-585.

Research output: Contribution to journalArticle

Chiou, SH, Kao, CL, Peng, CH, Chen, SJ, Tarng, YW, Ku, HH, Chen, YC, Shyr, YM, Liu, RS, Hsu, CJ, Yang, DM, Hsu, W-M, Kuo, CD & Lee, CH 2005, 'A novel in vitro retinal differentiation model by co-culturing adult human bone marrow stem cells with retinal pigmented epithelium cells', Biochemical and Biophysical Research Communications, vol. 326, no. 3, pp. 578-585. https://doi.org/10.1016/j.bbrc.2004.11.061
Chiou, Shih Hwa ; Kao, Chung Lan ; Peng, Chi Hsien ; Chen, Shih Jen ; Tarng, Yih Wen ; Ku, Hung Hai ; Chen, Yu Chih ; Shyr, Yi Ming ; Liu, Ren Shyan ; Hsu, Chien Jen ; Yang, De Ming ; Hsu, Wen-Ming ; Kuo, Cheng Deng ; Lee, Chen Hsen. / A novel in vitro retinal differentiation model by co-culturing adult human bone marrow stem cells with retinal pigmented epithelium cells. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 326, No. 3. pp. 578-585.
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