A novel function of the lysophosphatidic acid receptor 3 (LPAR3) gene in zebrafish on modulating anxiety, circadian rhythm locomotor activity, and short-term memory

Yu Nung Lin, Gilbert Audira, Nemi Malhotra, Nguyen Thi Ngoc Anh, Petrus Siregar, Jen Her Lu, Hsinyu Lee, Chung Der Hsiao

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Lysophosphatidic acid (LPA) is a small lysophospholipid molecule that activates multiple cellular functions through pathways with G-protein-coupled receptors. So far, six LPA receptors (LPAR1 to LPAR6) have been discovered and each one of them can connect to the downstream cell message-transmitting network. A previous study demonstrated that LPA receptors found in blood-producing stem cells can enhance erythropoietic processes through the activation of LPAR3. In the current study, newly discovered functions of LPAR3 were identified through extensive behavioral tests in lpar3 knockout (KO) zebrafish. It was found that the adult lpar3 KO zebrafish display an abnormal movement orientation and altered exploratory behavior compared to that of the control group in the three-dimensional locomotor and novel tank tests, respectively. Furthermore, consistent with those results, in the circadian rhythm locomotor activity test, the lpar3 KO zebrafish showed a lower level of angular velocity and average speed during the light cycles, indicating an hyperactivity-like behavior. In addition, the mutant fish also exhibited considerably higher locomotor activity during the dark cycle. Supporting those findings, this phenomenon was also displayed in the lpar3 KO zebrafish larvae. Furthermore, several important behavior alterations were also observed in the adult lpar3 KO fish, including a lower degree of aggression, less interest in conspecific social interaction, and looser shoal formation. However, there was no significant difference regarding the predator avoidance behavior between the mutant and the control fish. In addition, lpar3 KO zebrafish displayed memory deficiency in the passive avoidance test. These in vivo results support for the first time that the lpar3 gene plays a novel role in modulating behaviors of anxiety, aggression, social interaction, circadian rhythm locomotor activity, and memory retention in zebrafish.

Original languageEnglish
Article number2837
JournalInternational journal of molecular sciences
Volume21
Issue number8
DOIs
Publication statusPublished - Apr 2 2020
Externally publishedYes

Keywords

  • Anxiety
  • Behavior
  • Circadian rhythm locomotor activity
  • Lysophosphatidic acid receptor
  • Memory
  • Zebrafish

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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