A novel detection platform for shrimp white spot syndrome virus using an ICP11-dependent immunomagnetic reduction (IMR) assay

Bing Hsien Liu, Yu Chen Lin, Chia Shin Ho, Che Chuan Yang, Yun Tsui Chang, Jui Feng Chang, Chun Yuan Li, Cheng Shun Cheng, Jiun Yan Huang, Yen Fu Lee, Ming Hung Hsu, Feng Chun Lin, Hao Ching Wang, Chu Fang Lo, Shieh Yueh Yang, Han Ching Wang

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Abstract

Shrimp white spot disease (WSD), which is caused by white spot syndrome virus (WSSV), is one of the world's most serious shrimp diseases. Our objective in this study was to use an immunomagnetic reduction (IMR) assay to develop a highly sensitive, automatic WSSV detection platform targeted against ICP11 (the most highly expressed WSSV protein). After characterizing the magnetic reagents (Fe3O4 magnetic nanoparticles coated with anti ICP11), the detection limit for ICP11 protein using IMR was approximately 2 x 103 ng/ml, and the linear dynamic range of the assay was 0.1~1 x 106 ng/ml. In assays of ICP11 protein in pleopod protein lysates from healthy and WSSV-infected shrimp, IMR signals were successfully detected from shrimp with low WSSV genome copy numbers. We concluded that this IMR assay targeting ICP11 has potential for detecting the WSSV.

Original languageEnglish
Article numbere0138207
JournalPLoS One
Volume10
Issue number9
DOIs
Publication statusPublished - Sep 18 2015

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Liu, B. H., Lin, Y. C., Ho, C. S., Yang, C. C., Chang, Y. T., Chang, J. F., Li, C. Y., Cheng, C. S., Huang, J. Y., Lee, Y. F., Hsu, M. H., Lin, F. C., Wang, H. C., Lo, C. F., Yang, S. Y., & Wang, H. C. (2015). A novel detection platform for shrimp white spot syndrome virus using an ICP11-dependent immunomagnetic reduction (IMR) assay. PLoS One, 10(9), [e0138207]. https://doi.org/10.1371/journal.pone.0138207