A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid

Kuan Chung Chen, Ching Yu Lin, Cheng Chun Kuan, Hsien Yi Sung, Ching San Chen

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

A cDNA encoding a small cysteine-rich protein designated VrCRP was isolated from a bruchid-resistant mungbean. VrCRP encodes a protein of 73 amino acids containing a 27 amino acid signal peptide and 8 cysteines. On the basis of the amino acid sequence similarity and conserved residues, it is suggested that VrCRP is a member of the plant defensin family. VrCRP protein was obtained by overexpression of VrCRP with a truncated signal peptide in an IMPACT system. Artificial seeds containing 0.2% (w/w) of the purified VrCRP-TSP were lethal to larvae of the bruchid Callosobruchus chinensis. VrCRP is apparently the first reported plant defensin exhibiting in vitro insecticidal activity against C. chinensis.

Original languageEnglish
Pages (from-to)7258-7263
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume50
Issue number25
DOIs
Publication statusPublished - Dec 4 2002
Externally publishedYes

Fingerprint

Defensins
mung beans
Callosobruchus chinensis
Complementary DNA
Protein Sorting Signals
signal peptide
Amino Acids
Cysteine
cysteine
artificial seeds
Proteins
amino acids
proteins
lethal genes
Larva
Seed
Amino Acid Sequence
Seeds
amino acid sequences
larvae

Keywords

  • Bruchid resistance
  • Defensin
  • In vitro translation
  • Mungbean

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid. / Chen, Kuan Chung; Lin, Ching Yu; Kuan, Cheng Chun; Sung, Hsien Yi; Chen, Ching San.

In: Journal of Agricultural and Food Chemistry, Vol. 50, No. 25, 04.12.2002, p. 7258-7263.

Research output: Contribution to journalArticle

Chen, Kuan Chung ; Lin, Ching Yu ; Kuan, Cheng Chun ; Sung, Hsien Yi ; Chen, Ching San. / A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid. In: Journal of Agricultural and Food Chemistry. 2002 ; Vol. 50, No. 25. pp. 7258-7263.
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