A novel cationic cardiolipin analogue for gene delivery

Zhi Yi Zhang, S. Ugwu, A. Zhang, M. U. Ahmad, Imran Ahmad, S. Chiu, R. L. Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The optically active R and S isomers of cationic cardiolipin analogues (CCA) were synthesized and evaluated as a liposome based transfection reagent. Both isomers form stable liposomes with mean diameters of about 120nm without any additional lipid ingredients. No significant change in particle size distribution profile was observed over one-month storage at room temperature (20-25 °C). The gel to liquid crystalline phase transition temperature (Tm) of cationic liposomes comprised of both R and S isomers was approximately 2 °C, as measured by differential scanning calorimetry (DSC). Both isomers also formed stable liposomes when combined with DOPE. In vitro transfection efficiency of the CCA/DOPE liposomes complexed to plasmid DNA was evaluated using a luciferase reporter gene. Both liposomes composed of R and S isomers of the cationic cardiolipin displayed higher transfection efficiency than commercially available Lipofectin®. Further in vivo studies are warranted.

Original languageEnglish
Pages (from-to)10-14
Number of pages5
JournalPharmazie
Volume61
Issue number1
Publication statusPublished - Jan 2006
Externally publishedYes

Fingerprint

Cardiolipins
Liposomes
Isomers
Genes
Transfection
Transition Temperature
Phase Transition
Differential Scanning Calorimetry
Luciferases
Reporter Genes
Particle Size
Particle size analysis
Differential scanning calorimetry
Plasmids
Phase transitions
Gels
Crystalline materials
Lipids
Temperature
DNA

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Chemistry(all)
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science

Cite this

Zhang, Z. Y., Ugwu, S., Zhang, A., Ahmad, M. U., Ahmad, I., Chiu, S., & Lee, R. L. (2006). A novel cationic cardiolipin analogue for gene delivery. Pharmazie, 61(1), 10-14.

A novel cationic cardiolipin analogue for gene delivery. / Zhang, Zhi Yi; Ugwu, S.; Zhang, A.; Ahmad, M. U.; Ahmad, Imran; Chiu, S.; Lee, R. L.

In: Pharmazie, Vol. 61, No. 1, 01.2006, p. 10-14.

Research output: Contribution to journalArticle

Zhang, ZY, Ugwu, S, Zhang, A, Ahmad, MU, Ahmad, I, Chiu, S & Lee, RL 2006, 'A novel cationic cardiolipin analogue for gene delivery', Pharmazie, vol. 61, no. 1, pp. 10-14.
Zhang ZY, Ugwu S, Zhang A, Ahmad MU, Ahmad I, Chiu S et al. A novel cationic cardiolipin analogue for gene delivery. Pharmazie. 2006 Jan;61(1):10-14.
Zhang, Zhi Yi ; Ugwu, S. ; Zhang, A. ; Ahmad, M. U. ; Ahmad, Imran ; Chiu, S. ; Lee, R. L. / A novel cationic cardiolipin analogue for gene delivery. In: Pharmazie. 2006 ; Vol. 61, No. 1. pp. 10-14.
@article{1fe0651ec3e4489f976041e27ebe8c65,
title = "A novel cationic cardiolipin analogue for gene delivery",
abstract = "The optically active R and S isomers of cationic cardiolipin analogues (CCA) were synthesized and evaluated as a liposome based transfection reagent. Both isomers form stable liposomes with mean diameters of about 120nm without any additional lipid ingredients. No significant change in particle size distribution profile was observed over one-month storage at room temperature (20-25 °C). The gel to liquid crystalline phase transition temperature (Tm) of cationic liposomes comprised of both R and S isomers was approximately 2 °C, as measured by differential scanning calorimetry (DSC). Both isomers also formed stable liposomes when combined with DOPE. In vitro transfection efficiency of the CCA/DOPE liposomes complexed to plasmid DNA was evaluated using a luciferase reporter gene. Both liposomes composed of R and S isomers of the cationic cardiolipin displayed higher transfection efficiency than commercially available Lipofectin{\circledR}. Further in vivo studies are warranted.",
author = "Zhang, {Zhi Yi} and S. Ugwu and A. Zhang and Ahmad, {M. U.} and Imran Ahmad and S. Chiu and Lee, {R. L.}",
year = "2006",
month = "1",
language = "English",
volume = "61",
pages = "10--14",
journal = "Die Pharmazie",
issn = "0031-7144",
publisher = "Govi-Verlag Pharmazeutischer Verlag GmbH",
number = "1",

}

TY - JOUR

T1 - A novel cationic cardiolipin analogue for gene delivery

AU - Zhang, Zhi Yi

AU - Ugwu, S.

AU - Zhang, A.

AU - Ahmad, M. U.

AU - Ahmad, Imran

AU - Chiu, S.

AU - Lee, R. L.

PY - 2006/1

Y1 - 2006/1

N2 - The optically active R and S isomers of cationic cardiolipin analogues (CCA) were synthesized and evaluated as a liposome based transfection reagent. Both isomers form stable liposomes with mean diameters of about 120nm without any additional lipid ingredients. No significant change in particle size distribution profile was observed over one-month storage at room temperature (20-25 °C). The gel to liquid crystalline phase transition temperature (Tm) of cationic liposomes comprised of both R and S isomers was approximately 2 °C, as measured by differential scanning calorimetry (DSC). Both isomers also formed stable liposomes when combined with DOPE. In vitro transfection efficiency of the CCA/DOPE liposomes complexed to plasmid DNA was evaluated using a luciferase reporter gene. Both liposomes composed of R and S isomers of the cationic cardiolipin displayed higher transfection efficiency than commercially available Lipofectin®. Further in vivo studies are warranted.

AB - The optically active R and S isomers of cationic cardiolipin analogues (CCA) were synthesized and evaluated as a liposome based transfection reagent. Both isomers form stable liposomes with mean diameters of about 120nm without any additional lipid ingredients. No significant change in particle size distribution profile was observed over one-month storage at room temperature (20-25 °C). The gel to liquid crystalline phase transition temperature (Tm) of cationic liposomes comprised of both R and S isomers was approximately 2 °C, as measured by differential scanning calorimetry (DSC). Both isomers also formed stable liposomes when combined with DOPE. In vitro transfection efficiency of the CCA/DOPE liposomes complexed to plasmid DNA was evaluated using a luciferase reporter gene. Both liposomes composed of R and S isomers of the cationic cardiolipin displayed higher transfection efficiency than commercially available Lipofectin®. Further in vivo studies are warranted.

UR - http://www.scopus.com/inward/record.url?scp=31144435328&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=31144435328&partnerID=8YFLogxK

M3 - Article

VL - 61

SP - 10

EP - 14

JO - Die Pharmazie

JF - Die Pharmazie

SN - 0031-7144

IS - 1

ER -