A nontoxic Pseudomonas exotoxin A induces active immunity and passive protective antibody against Pseudomonas exotoxin A intoxication

Tzong Yueh Chen, Chia Po Lin, Chien Chang Loa, Tso Ling Chen, Huey Fang Shang, Jaulang Hwang, Cho Fat Hui

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Pseudomonas exotoxin A (PE) is one of the most potent cytotoxic agents produced by Pseudomonas aeruginosa. In this study, we examined the possibility of using PE with a deletion of 38 carboxyl-terminal amino acid residues, designated PE(ΔA576-613), for active immunization against PE- mediated disease. We first examined the toxic effects of PE and PE(Δ576-613) on 5- and 9-week-old ICR mice. The results show that the subcutaneous administration of PE(Δ576-613) at a dose of 250 μg was still nontoxic to 5- and 9-week-old ICR mice, while native PE was lethal at a dose of 0.5 and 1 μg, respectively. PE(Δ576-613) was then used to immunize ICR mice. The minimum dose of PE(Δ576-613) that could effectively induce anti-PE antibodies in 5- and 9-week-old ICR mice was found to be 250 ng. However, immunization with 250 ng PE(Δ576-613) failed to protect the immunized mice from a lethal dose of PE. The effective immunization dose of PE(Δ576-613) that could protect mice against a 2 μg PE challenge was found to be 15 μg. In addition, sera obtained from PE(Δ576-613)-immunized ICR mice were able to neutralize PE intoxication and effectively protect mice from PE. Thus, PE(Δ576-613) may be used as an alternative route to new PE vaccine development.

Original languageEnglish
Pages (from-to)357-363
Number of pages7
JournalJournal of Biomedical Science
Volume6
Issue number5
Publication statusPublished - Sep 1999

Keywords

  • Pseudomonas exotoxin A
  • Vaccination

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'A nontoxic Pseudomonas exotoxin A induces active immunity and passive protective antibody against Pseudomonas exotoxin A intoxication'. Together they form a unique fingerprint.

  • Cite this

    Chen, T. Y., Lin, C. P., Loa, C. C., Chen, T. L., Shang, H. F., Hwang, J., & Hui, C. F. (1999). A nontoxic Pseudomonas exotoxin A induces active immunity and passive protective antibody against Pseudomonas exotoxin A intoxication. Journal of Biomedical Science, 6(5), 357-363.