A new in vitro method of decongestant assay of nasal mucosa

A preliminary report

Hsing Won Wang, Chuan Hsiang Kao, Yueng Hsiang Chu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: The calculation of cell planar surface area, successfully used for glomerular mesangial cell-altered contractility, also is a reasonable methodology to assess nasal mucosal contractility. A simple technique that works particularly well with vasoconstriction-inducing agents has been introduced to test the effects of several drugs on patient turbinate nasal mucosa in vitro. This report will introduce this technique and present some of the initial findings. Methods: We tested epinephrine, norepinephrine, pseudoephedrine HCl, and methoxamine, respectively, for effectiveness as a vasoconstricting drug. We also tested the effectiveness of verapamil as a vasodilating drug. A piece of nasal mucosa ∼3 × 4 mm in size was mounted with a 27-gauge needle and submersed in a 4-mL muscle bath in a Petri dish at room temperature. The bath consisted of 4 mL of Kreb's solution. We recorded the decrease of nasal mucosa planar surface area in response to the application of vasoconstricting agents, as measured using a digital microscope connected to a Pentium III with morphometric software, as the estimated degree of contraction. A photograph of the same mucosa was taken every 3 minutes for a total period of 18 minutes. Results: Nasal mucosa that was mounted in a muscle bath in a Petri dish and treated with a small dose of a nasal decongestant was observed to contract. Drug dosage determined the degree of nasal mucosa contraction. Nasal mucosa similarly prepared but treated with a vasodilating agent, such as verapamil, exhibited a relaxation response only when pretreated with a vasoconstricting agent, such as methoxamine. Both vasoconstricting and vasodilating drugs induce dose-related responses in planar surface calculation. Conclusion: In this study, drug dosage determined the degree of nasal mucosa contraction. We also found that the rate of contraction and percent of contraction were dose dependent. We believe this new method will prove useful in studying the effects of drugs on nasal blood vessels.

Original languageEnglish
Pages (from-to)43-47
Number of pages5
JournalAmerican Journal of Rhinology
Volume20
Issue number1
Publication statusPublished - Jan 2006
Externally publishedYes

Fingerprint

Nasal Decongestants
Nasal Mucosa
Pharmaceutical Preparations
Baths
Methoxamine
Verapamil
Nose
Pseudoephedrine
Muscles
Turbinates
Mesangial Cells
In Vitro Techniques
Vasoconstriction
Epinephrine
Needles
Blood Vessels
Norepinephrine
Mucous Membrane
Software
Temperature

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

A new in vitro method of decongestant assay of nasal mucosa : A preliminary report. / Wang, Hsing Won; Kao, Chuan Hsiang; Chu, Yueng Hsiang.

In: American Journal of Rhinology, Vol. 20, No. 1, 01.2006, p. 43-47.

Research output: Contribution to journalArticle

Wang, Hsing Won ; Kao, Chuan Hsiang ; Chu, Yueng Hsiang. / A new in vitro method of decongestant assay of nasal mucosa : A preliminary report. In: American Journal of Rhinology. 2006 ; Vol. 20, No. 1. pp. 43-47.
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