A Mutation in Cartilage Oligomeric Matrix Protein (COMP) Causes Early-Onset Osteoarthritis in a Large Kindred Study

Shu Chi Mu, Yi Jung Lin, Hwa Chang Liu, Jer Yuarn Wu, Sing Chung Li, Ming Ta Michael Lee, Ching Heng Chou, Liang Kuang Chen, Yuan Tsong Chen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We performed a genome-wide linkage analysis to identify susceptibility loci in a large six-generation extended family previously reported with early-onset osteoarthritis (OA) DNA sequencing was performed to investigate involvement of the COMP (Cartilage oligomeric matrix protein) gene in this family. The region covering D19S884, D19S226, and D19S414 on chromosome 19p following genome-wide scan from 70 individuals of this kindred showed significant linkage, with a maximum point LOD (logarithm of the odds ratio) score of 2.51 at D19S226. Direct sequencing of the COMP gene, the most plausible candidate gene in the region, identified a c.2152C>T substitution in exon 18 which resulted in a substitution of tryptophan for arginine at position 718 located in the C terminal globular domain of the gene product. A total of 26 individuals were identified with this mutation of which 21 affected individuals had the mutation, and the other five younger individuals (18.6 ± 11.3 years of age) carried the mutation without symptoms. The results indicate that COMP is the disease susceptibility gene and the c.2152C>T mutation in exon 18 could cause early-onset OA phenotypes in this kindred, which is compatible with a previous report that this mutation also causes a mild form of multiple epiphyseal dysplasia (MED).

Original languageEnglish
Pages (from-to)575-583
Number of pages9
JournalAnnals of Human Genetics
Volume75
Issue number5
DOIs
Publication statusPublished - Sep 2011
Externally publishedYes

Fingerprint

Cartilage Oligomeric Matrix Protein
Osteoarthritis
Mutation
Genes
Exons
Genome
Osteochondrodysplasias
Disease Susceptibility
DNA Sequence Analysis
Tryptophan
Arginine
Chromosomes
Odds Ratio
Phenotype

Keywords

  • COMP
  • Early-onset osteoarthritis
  • Genome-wide linkage
  • Multiple epiphyseal dysplasia (MED)
  • Phenotype
  • Pseudoachondroplasia (PSACH)

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

A Mutation in Cartilage Oligomeric Matrix Protein (COMP) Causes Early-Onset Osteoarthritis in a Large Kindred Study. / Mu, Shu Chi; Lin, Yi Jung; Liu, Hwa Chang; Wu, Jer Yuarn; Li, Sing Chung; Michael Lee, Ming Ta; Chou, Ching Heng; Chen, Liang Kuang; Chen, Yuan Tsong.

In: Annals of Human Genetics, Vol. 75, No. 5, 09.2011, p. 575-583.

Research output: Contribution to journalArticle

Mu, Shu Chi ; Lin, Yi Jung ; Liu, Hwa Chang ; Wu, Jer Yuarn ; Li, Sing Chung ; Michael Lee, Ming Ta ; Chou, Ching Heng ; Chen, Liang Kuang ; Chen, Yuan Tsong. / A Mutation in Cartilage Oligomeric Matrix Protein (COMP) Causes Early-Onset Osteoarthritis in a Large Kindred Study. In: Annals of Human Genetics. 2011 ; Vol. 75, No. 5. pp. 575-583.
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