A multiplexed quantitative strategy for membrane proteomics: Opportunities for mining therapeutic targets for autosomal dominant polycystic kidney disease

Chia Li Han, Chih Wei Chien, Wen Cheng Chen, Yet Ran Chen, Chien Peng Wu, Hung Li, Yu Ju Chen

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Toward multiplexed, comprehensive, and robust quantitation of the membrane proteome, we report a strategy combining gel-assisted digestion, iTRAQ (isobaric tags for relative and absolute quantitation) labeling, and LCMS/MS. Quantitation of four independently purified membrane fractions from HeLa cells gave high accuracy (

Original languageEnglish
Pages (from-to)1983-1997
Number of pages15
JournalMolecular and Cellular Proteomics
Volume7
Issue number10
DOIs
Publication statusPublished - Oct 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

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