A multiplexed quantitative strategy for membrane proteomics

Opportunities for mining therapeutic targets for autosomal dominant polycystic kidney disease

Chia Li Han, Chih Wei Chien, Wen Cheng Chen, Yet Ran Chen, Chien Peng Wu, Hung Li, Yu Ju Chen

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Toward multiplexed, comprehensive, and robust quantitation of the membrane proteome, we report a strategy combining gel-assisted digestion, iTRAQ (isobaric tags for relative and absolute quantitation) labeling, and LCMS/MS. Quantitation of four independently purified membrane fractions from HeLa cells gave high accuracy (

Original languageEnglish
Pages (from-to)1983-1997
Number of pages15
JournalMolecular and Cellular Proteomics
Volume7
Issue number10
DOIs
Publication statusPublished - Oct 2008
Externally publishedYes

Fingerprint

Autosomal Dominant Polycystic Kidney
Proteomics
Membranes
Proteome
HeLa Cells
Labeling
Digestion
Gels
Therapeutics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Cite this

A multiplexed quantitative strategy for membrane proteomics : Opportunities for mining therapeutic targets for autosomal dominant polycystic kidney disease. / Han, Chia Li; Chien, Chih Wei; Chen, Wen Cheng; Chen, Yet Ran; Wu, Chien Peng; Li, Hung; Chen, Yu Ju.

In: Molecular and Cellular Proteomics, Vol. 7, No. 10, 10.2008, p. 1983-1997.

Research output: Contribution to journalArticle

Han, Chia Li ; Chien, Chih Wei ; Chen, Wen Cheng ; Chen, Yet Ran ; Wu, Chien Peng ; Li, Hung ; Chen, Yu Ju. / A multiplexed quantitative strategy for membrane proteomics : Opportunities for mining therapeutic targets for autosomal dominant polycystic kidney disease. In: Molecular and Cellular Proteomics. 2008 ; Vol. 7, No. 10. pp. 1983-1997.
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