A multiplex polymerase chain reaction method for rapid identification of Citrobacter freundii and Salmonella species, including Salmonella Typhi

Chia G. Lin, Cheng Hsun Chiu, Chishih Chu, Yhu Chering Huang, Tzou Yien Lin, Jonathan T. Ou

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background and Purpose: Salmonella enterica is one of the most common enteric pathogens worldwide. Conventional methods of isolation of Salmonella strains take 4-7 days to complete, are laborious and require substantial manpower. We devised a polymerase chain reaction (PCR) method that simultaneously uses three pairs of specific primers to detect inv, spv, and via genes of Salmonella. Methods: Three primer pairs were designed, including: SPVC-1 and SPVC-2, based on the nucleotide sequences of the spvC gene; INVA-1 and INVA-2, based on the invA gene; and VIAB-1 and VIAB-2, based on the viaB gene. PCR was performed using these three primers to identify 14 clinically important bacterial organisms, including Citrobacter freundii, S. enterica serovars Typhi and Paratyphi C, Dublin, and other non-typhoidal Salmonella that harbor a virulence plasmid. Results: The following strains were readily identified using the PCR: (1) C. freundii; (2) S. Typhi; and S. Paratyphi C; (3) S. Dublin (virulence antigen-positive); and (4) Salmonella serovars that harbor an spy-type virulence plasmid. S. enterica could also be identified, but required further testing to determine serovar. Conclusions: This PCR method allows S. Typhi to be identified immediately so that appropriate antibiotic treatment can be initiated without delay.

Original languageEnglish
Pages (from-to)222-226
Number of pages5
JournalJournal of Microbiology, Immunology and Infection
Volume40
Issue number3
Publication statusPublished - Jun 2007
Externally publishedYes

Fingerprint

Citrobacter freundii
Salmonella typhi
Multiplex Polymerase Chain Reaction
Salmonella
Virulence
Polymerase Chain Reaction
Salmonella enterica
Salmonella paratyphi C
Genes
Plasmids
Anti-Bacterial Agents
Antigens
Serogroup

Keywords

  • Citrobacter freundii
  • Polymerase chain reaction
  • Salmonella enterica

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Immunology and Microbiology(all)

Cite this

A multiplex polymerase chain reaction method for rapid identification of Citrobacter freundii and Salmonella species, including Salmonella Typhi. / Lin, Chia G.; Chiu, Cheng Hsun; Chu, Chishih; Huang, Yhu Chering; Lin, Tzou Yien; Ou, Jonathan T.

In: Journal of Microbiology, Immunology and Infection, Vol. 40, No. 3, 06.2007, p. 222-226.

Research output: Contribution to journalArticle

@article{e2dba2ee63ac4785ae013aa2e6561693,
title = "A multiplex polymerase chain reaction method for rapid identification of Citrobacter freundii and Salmonella species, including Salmonella Typhi",
abstract = "Background and Purpose: Salmonella enterica is one of the most common enteric pathogens worldwide. Conventional methods of isolation of Salmonella strains take 4-7 days to complete, are laborious and require substantial manpower. We devised a polymerase chain reaction (PCR) method that simultaneously uses three pairs of specific primers to detect inv, spv, and via genes of Salmonella. Methods: Three primer pairs were designed, including: SPVC-1 and SPVC-2, based on the nucleotide sequences of the spvC gene; INVA-1 and INVA-2, based on the invA gene; and VIAB-1 and VIAB-2, based on the viaB gene. PCR was performed using these three primers to identify 14 clinically important bacterial organisms, including Citrobacter freundii, S. enterica serovars Typhi and Paratyphi C, Dublin, and other non-typhoidal Salmonella that harbor a virulence plasmid. Results: The following strains were readily identified using the PCR: (1) C. freundii; (2) S. Typhi; and S. Paratyphi C; (3) S. Dublin (virulence antigen-positive); and (4) Salmonella serovars that harbor an spy-type virulence plasmid. S. enterica could also be identified, but required further testing to determine serovar. Conclusions: This PCR method allows S. Typhi to be identified immediately so that appropriate antibiotic treatment can be initiated without delay.",
keywords = "Citrobacter freundii, Polymerase chain reaction, Salmonella enterica",
author = "Lin, {Chia G.} and Chiu, {Cheng Hsun} and Chishih Chu and Huang, {Yhu Chering} and Lin, {Tzou Yien} and Ou, {Jonathan T.}",
year = "2007",
month = "6",
language = "English",
volume = "40",
pages = "222--226",
journal = "Journal of Microbiology, Immunology and Infection",
issn = "0253-2662",
publisher = "Elsevier Taiwan LLC",
number = "3",

}

TY - JOUR

T1 - A multiplex polymerase chain reaction method for rapid identification of Citrobacter freundii and Salmonella species, including Salmonella Typhi

AU - Lin, Chia G.

AU - Chiu, Cheng Hsun

AU - Chu, Chishih

AU - Huang, Yhu Chering

AU - Lin, Tzou Yien

AU - Ou, Jonathan T.

PY - 2007/6

Y1 - 2007/6

N2 - Background and Purpose: Salmonella enterica is one of the most common enteric pathogens worldwide. Conventional methods of isolation of Salmonella strains take 4-7 days to complete, are laborious and require substantial manpower. We devised a polymerase chain reaction (PCR) method that simultaneously uses three pairs of specific primers to detect inv, spv, and via genes of Salmonella. Methods: Three primer pairs were designed, including: SPVC-1 and SPVC-2, based on the nucleotide sequences of the spvC gene; INVA-1 and INVA-2, based on the invA gene; and VIAB-1 and VIAB-2, based on the viaB gene. PCR was performed using these three primers to identify 14 clinically important bacterial organisms, including Citrobacter freundii, S. enterica serovars Typhi and Paratyphi C, Dublin, and other non-typhoidal Salmonella that harbor a virulence plasmid. Results: The following strains were readily identified using the PCR: (1) C. freundii; (2) S. Typhi; and S. Paratyphi C; (3) S. Dublin (virulence antigen-positive); and (4) Salmonella serovars that harbor an spy-type virulence plasmid. S. enterica could also be identified, but required further testing to determine serovar. Conclusions: This PCR method allows S. Typhi to be identified immediately so that appropriate antibiotic treatment can be initiated without delay.

AB - Background and Purpose: Salmonella enterica is one of the most common enteric pathogens worldwide. Conventional methods of isolation of Salmonella strains take 4-7 days to complete, are laborious and require substantial manpower. We devised a polymerase chain reaction (PCR) method that simultaneously uses three pairs of specific primers to detect inv, spv, and via genes of Salmonella. Methods: Three primer pairs were designed, including: SPVC-1 and SPVC-2, based on the nucleotide sequences of the spvC gene; INVA-1 and INVA-2, based on the invA gene; and VIAB-1 and VIAB-2, based on the viaB gene. PCR was performed using these three primers to identify 14 clinically important bacterial organisms, including Citrobacter freundii, S. enterica serovars Typhi and Paratyphi C, Dublin, and other non-typhoidal Salmonella that harbor a virulence plasmid. Results: The following strains were readily identified using the PCR: (1) C. freundii; (2) S. Typhi; and S. Paratyphi C; (3) S. Dublin (virulence antigen-positive); and (4) Salmonella serovars that harbor an spy-type virulence plasmid. S. enterica could also be identified, but required further testing to determine serovar. Conclusions: This PCR method allows S. Typhi to be identified immediately so that appropriate antibiotic treatment can be initiated without delay.

KW - Citrobacter freundii

KW - Polymerase chain reaction

KW - Salmonella enterica

UR - http://www.scopus.com/inward/record.url?scp=34948904276&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34948904276&partnerID=8YFLogxK

M3 - Article

VL - 40

SP - 222

EP - 226

JO - Journal of Microbiology, Immunology and Infection

JF - Journal of Microbiology, Immunology and Infection

SN - 0253-2662

IS - 3

ER -