A molecular approach applied to enteroviruses surveillance in Northern Taiwan, 2008-2012

Wan Yu Chung, Pai Shan Chiang, Shu Ting Luo, Tzou Yien Lin, Kuo Chien Tsao, Min Shi Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Traditional methods for detection and serotyping of enterovirus infections are virus isolation and immunofluorescence assay (VI-IFA), which are labor-intensive and time-consuming. Recently, VP1 gene has been targeted to develop a CODEHOP-based RT-PCR (VP1- CODEHOP) for the same purpose. In this study, we conducted a 5-year enterovirus surveillance comparing the VI-IFA and VP1-CODEHOP tests. Throat swabs were collected from 431 pediatric patients and 208(48%) and 250(58%) were tested positive by the VI-IFA and VP1-CODEHOP tests, respectively. Among the 47 cases who had inconsistent results between the VI-IFA and VP1-CODEHOP tests and provided paired sera for serological verifications, correct diagnosis for the VI-IFA and VP1-CODEHOP were 5(11%) and 40(85%) cases, respectively. Therefore, the VP1-CODEHOP is more reliable for detection of human enteroviruses than the VI-IFA. Based on serological verifications for the eight cases who had inconsistent serotypes between the two tests and provided paired sera, five and two showed consistent serotypes with the VP1-CODEHOP and VI-IFA tests, respectively. CVA16, CVA6 and EV71 were the most prevalent serotypes in northern Taiwan, 2008~2012. Moreover, variant CVA2, CVA6 and EV71 viruses were further identified based on phylogenetic analysis of partial VP1 sequences. In conclusion, the VP1-CODEHOP test could be used as the primary method for enterovirus surveillance to support decision-making for outbreak control.

Original languageEnglish
Article numbere0167532
JournalPLoS One
Volume11
Issue number12
DOIs
Publication statusPublished - Dec 1 2016
Externally publishedYes

Fingerprint

Enterovirus
Taiwan
Viruses
fluorescent antibody technique
Fluorescent Antibody Technique
Assays
viruses
monitoring
assays
serotypes
testing
Human enterovirus
Enterovirus Infections
Serotyping
Pediatrics
throat
Pharynx
Serum
Disease Outbreaks
decision making

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

A molecular approach applied to enteroviruses surveillance in Northern Taiwan, 2008-2012. / Chung, Wan Yu; Chiang, Pai Shan; Luo, Shu Ting; Lin, Tzou Yien; Tsao, Kuo Chien; Lee, Min Shi.

In: PLoS One, Vol. 11, No. 12, e0167532, 01.12.2016.

Research output: Contribution to journalArticle

Chung, Wan Yu ; Chiang, Pai Shan ; Luo, Shu Ting ; Lin, Tzou Yien ; Tsao, Kuo Chien ; Lee, Min Shi. / A molecular approach applied to enteroviruses surveillance in Northern Taiwan, 2008-2012. In: PLoS One. 2016 ; Vol. 11, No. 12.
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abstract = "Traditional methods for detection and serotyping of enterovirus infections are virus isolation and immunofluorescence assay (VI-IFA), which are labor-intensive and time-consuming. Recently, VP1 gene has been targeted to develop a CODEHOP-based RT-PCR (VP1- CODEHOP) for the same purpose. In this study, we conducted a 5-year enterovirus surveillance comparing the VI-IFA and VP1-CODEHOP tests. Throat swabs were collected from 431 pediatric patients and 208(48{\%}) and 250(58{\%}) were tested positive by the VI-IFA and VP1-CODEHOP tests, respectively. Among the 47 cases who had inconsistent results between the VI-IFA and VP1-CODEHOP tests and provided paired sera for serological verifications, correct diagnosis for the VI-IFA and VP1-CODEHOP were 5(11{\%}) and 40(85{\%}) cases, respectively. Therefore, the VP1-CODEHOP is more reliable for detection of human enteroviruses than the VI-IFA. Based on serological verifications for the eight cases who had inconsistent serotypes between the two tests and provided paired sera, five and two showed consistent serotypes with the VP1-CODEHOP and VI-IFA tests, respectively. CVA16, CVA6 and EV71 were the most prevalent serotypes in northern Taiwan, 2008~2012. Moreover, variant CVA2, CVA6 and EV71 viruses were further identified based on phylogenetic analysis of partial VP1 sequences. In conclusion, the VP1-CODEHOP test could be used as the primary method for enterovirus surveillance to support decision-making for outbreak control.",
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