A minimal stress model for the assessment of electroacupuncture analgesia in rats under halothane

Yeong-Ray Wen, Geng Chang Yeh, Bai Chuang Shyu, Qing Dong Ling, Kuo Ching Wang, Ta Liang Chen, Wei Zen Sun

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The use of anesthetics in acupuncture analgesia is controversial. We evaluate a steady-state light anesthesia model to test whether minimal stress manipulation and reliable measurement of analgesia could be simultaneously achieved during electroacupuncture (EA) in animals. A series of experiments were performed. Firstly, EA compliance and tail-flick latencies (TFL) were compared in rats under 0.1%, 0.3%, 0.5%, 0.7%, or 1.1% halothane for 120 min. Under 0.5% halothane, TFL were then measured in groups receiving EA at intensity of 3, 10 or 20 volt (V), 1 or 2 mg/kg morphine, 20 V EA plus naloxone, or control. Subsequently, the effect of EA on formalin-induced hyperalgesia was tested and c-fos expression in the spinal dorsal horn was analyzed. Rats exhibited profound irritable behaviors and highly variable TFL under 0.1% or 0.3% halothane, as well as a time-dependent increase of TFL under 0.7% or 1.1% halothane. TFL remained constant at 0.5% halothane, and needle insertion and electrical stimulation were well tolerated. Under 0.5% halothane, EA increased TFL and suppressed formalin-induced hyperalgesia in an intensity-dependent and naloxone-reversible manner. EA of 20 V prolonged TFL by 74%, suppressed formalin-induced hyperalgesia by 32.6% and decreased c-fos expression by 29.7% at the superficial and deep dorsal horn with statistically significant difference. In conclusion, 0.5% halothane provides a steady-state anesthetic level which enables the humane application of EA stimulus with the least interference on analgesic assessment. This condition serves as a minimal stress EA model in animals devoid of stress-induced analgesia while maintaining physiological and biochemical response in the experiment.

Original languageEnglish
Pages (from-to)733-742
Number of pages10
JournalEuropean Journal of Pain (United Kingdom)
Volume11
Issue number7
DOIs
Publication statusPublished - Oct 2007

Fingerprint

Electroacupuncture
Halothane
Analgesia
Tail
Hyperalgesia
Formaldehyde
Naloxone
Anesthetics
Acupuncture Analgesia
Morphine
Electric Stimulation
Needles
Analgesics
Anesthesia
Animal Models

Keywords

  • c-Fos
  • Electroacupuncture
  • Formalin
  • Halothane
  • Model

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine
  • Neurology
  • Neuropsychology and Physiological Psychology

Cite this

A minimal stress model for the assessment of electroacupuncture analgesia in rats under halothane. / Wen, Yeong-Ray; Yeh, Geng Chang; Shyu, Bai Chuang; Ling, Qing Dong; Wang, Kuo Ching; Chen, Ta Liang; Sun, Wei Zen.

In: European Journal of Pain (United Kingdom), Vol. 11, No. 7, 10.2007, p. 733-742.

Research output: Contribution to journalArticle

Wen, Yeong-Ray ; Yeh, Geng Chang ; Shyu, Bai Chuang ; Ling, Qing Dong ; Wang, Kuo Ching ; Chen, Ta Liang ; Sun, Wei Zen. / A minimal stress model for the assessment of electroacupuncture analgesia in rats under halothane. In: European Journal of Pain (United Kingdom). 2007 ; Vol. 11, No. 7. pp. 733-742.
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AU - Chen, Ta Liang

AU - Sun, Wei Zen

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