A Microtube Array Membrane (MTAM) Encapsulated Live Fermenting Staphylococcus epidermidis as a Skin Probiotic Patch against Cutibacterium acnes

Albert-Jackson Yang, Shinta Marito, John-Jackson Yang, Sunita Keshari, Chee-Ho Chew, Chien-Chung Chen, Chun-Ming Huang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Antibiotics without selectivity for acne treatment may destroy the beneficial microbes in the human microbiome that helps to fight Cutibacterium acnes (C. acnes), a bacterium associated with inflammatory acne vulgaris. Probiotic treatment by direct application of live Staphylococcus epidermidis (S. epidermidis) onto the open acne lesions may run the risk of bloodstream infections. Here, we fabricated the polysulfone microtube array membranes (PSF MTAM) to encapsulate probiotic S.epidermidis. We demonstrate that the application of the encapsulation of S.epidermidis in PSF MTAM enhanced the glycerol fermentation activities of S. epidermidis. To mimic the granulomatous type of acne inflammatory acne vulgaris, the ears of mice were injected intradermally with C.acnes to induce the secretion of macrophage inflammatory protein-2 (MIP-2), a murine counterpart of human interleukin (IL)-8. The C. acnes-injected mouse ears were covered with a PST MTAM encapsulated with or without S.epidermidis in the presence of glycerol. The application of S.epidermidis-encapsulated PST MTAM plus glycerol onto the C.acnes-injected mouse ears considerably reduced the growth of C. acnes and the production of MIP-2. Furthermore, no S. epidermidis leaked from PSF MTAM into mouse skin. The S. epidermidis-encapsulated PST MTAM functions as a probiotic acne patch.

Original languageEnglish
JournalInternational Journal of Molecular Sciences
Volume20
Issue number1
DOIs
Publication statusPublished - Dec 20 2018

Fingerprint

Staphylococcus epidermidis
staphylococcus
Acne Vulgaris
Probiotics
Skin
membranes
Membranes
Polysulfones
mice
ear
glycerols
Glycerol
Chemokine CXCL2
macrophages
Macrophages
Ear
interleukins
proteins
Proteins
secretions

Keywords

  • Animals
  • Antibiosis
  • Chemokine CXCL2/metabolism
  • Dermatitis/metabolism
  • Fermentation
  • Glycerol/metabolism
  • Humans
  • Mice
  • Probiotics
  • Propionibacteriaceae/physiology
  • Skin/microbiology
  • Staphylococcus epidermidis/physiology

Cite this

A Microtube Array Membrane (MTAM) Encapsulated Live Fermenting Staphylococcus epidermidis as a Skin Probiotic Patch against Cutibacterium acnes. / Yang, Albert-Jackson; Marito, Shinta; Yang, John-Jackson; Keshari, Sunita; Chew, Chee-Ho; Chen, Chien-Chung; Huang, Chun-Ming.

In: International Journal of Molecular Sciences, Vol. 20, No. 1, 20.12.2018.

Research output: Contribution to journalArticle

Yang, Albert-Jackson ; Marito, Shinta ; Yang, John-Jackson ; Keshari, Sunita ; Chew, Chee-Ho ; Chen, Chien-Chung ; Huang, Chun-Ming. / A Microtube Array Membrane (MTAM) Encapsulated Live Fermenting Staphylococcus epidermidis as a Skin Probiotic Patch against Cutibacterium acnes. In: International Journal of Molecular Sciences. 2018 ; Vol. 20, No. 1.
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abstract = "Antibiotics without selectivity for acne treatment may destroy the beneficial microbes in the human microbiome that helps to fight Cutibacterium acnes (C. acnes), a bacterium associated with inflammatory acne vulgaris. Probiotic treatment by direct application of live Staphylococcus epidermidis (S. epidermidis) onto the open acne lesions may run the risk of bloodstream infections. Here, we fabricated the polysulfone microtube array membranes (PSF MTAM) to encapsulate probiotic S.epidermidis. We demonstrate that the application of the encapsulation of S.epidermidis in PSF MTAM enhanced the glycerol fermentation activities of S. epidermidis. To mimic the granulomatous type of acne inflammatory acne vulgaris, the ears of mice were injected intradermally with C.acnes to induce the secretion of macrophage inflammatory protein-2 (MIP-2), a murine counterpart of human interleukin (IL)-8. The C. acnes-injected mouse ears were covered with a PST MTAM encapsulated with or without S.epidermidis in the presence of glycerol. The application of S.epidermidis-encapsulated PST MTAM plus glycerol onto the C.acnes-injected mouse ears considerably reduced the growth of C. acnes and the production of MIP-2. Furthermore, no S. epidermidis leaked from PSF MTAM into mouse skin. The S. epidermidis-encapsulated PST MTAM functions as a probiotic acne patch.",
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KW - Mice

KW - Probiotics

KW - Propionibacteriaceae/physiology

KW - Skin/microbiology

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