A microfluidic labchip for angiogenesis inhibitor studies via multi-gradients of cancer and fibroblast stimuli

Cheng Hao Chin, Yi Liang Chiu, Kang Yun Lee, Cheng Hsien Liu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Recently, the study of in vitro capillary network using microfluidic devices by generating single concentration gradient have uncovered many biological information of angiogenesis. However, the platform for angiogenesis inhibitors have not been proposed. In this study, we have developed a microfluidic device for generation of multi gradients to stimuli cancer-induced angiogenesis and fibroblast-induced angiogenesis simultaneously. The device features an interaction chamber, connected to the surrounding channels with radian shaped low-height structures used to locate clusters of cells. The formation of multiple gradients is achieved in the interaction chamber through diffusion and sustained by continuous perfusion. Microfluidic valve was developed to control the direction of flow for trapping cells of different types respectively. We show that our device could generate multiple concentration gradient and trap clusters of cells. These results demonstrated that our device is able to stimuli the angiogenesis and can be used to do the angiogenesis inhibitor study.

Original languageEnglish
Title of host publication2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages363-366
Number of pages4
ISBN (Print)9781479989553
DOIs
Publication statusPublished - Aug 5 2015
Event18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States
Duration: Jun 21 2015Jun 25 2015

Other

Other18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
CountryUnited States
CityAnchorage
Period6/21/156/25/15

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Keywords

  • Angiogenesis
  • cell manipulation
  • gradient generator
  • in vitro model

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Chin, C. H., Chiu, Y. L., Lee, K. Y., & Liu, C. H. (2015). A microfluidic labchip for angiogenesis inhibitor studies via multi-gradients of cancer and fibroblast stimuli. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 (pp. 363-366). [7180936] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2015.7180936