A mechanistic study on urine retention in D-amphetamine addicts

Hui-Fang Chiu, Jen-Kun Cheng, Pu-Hua Lin, Jung-Chen Chang, I. Ming Chen, Ching I. Lin, Wei Pin Chang, Chia-Hsien Lin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Chronic amphetamine intake leads to neurogenic bladder and chronic urinary retention. The mechanism underlying persistent urinary retention is unclear. The pelvic-urethral reflex (PUR) is essential for the urethra to develop sufficient resistance to maintain urine continence, an important function of the urinary system. Recent studies on PUR activities have indicated that repetitive/tetanic stimulation of the pelvic afferent fibers induces spinal reflex potentiation (SRP) in PUR activities, which further increases urinary retention. In this study, results showed that test stimulation (TS, 1/30 Hz) evoked a baseline reflex activity, while repetitive stimulation (RS, 1 Hz) induced reflex potentiation in the external urethral sphincter. Intrathecal d-amphetamine (AMPH, 30 μM) did not but higher AMPH concentration (100 μM) induced SRP in TS-induced reflex activity. H89 (10 μM, a protein kinase A inhibitor), but not chelerythrine chloride (CTC, 10 μM, a protein kinase C inhibitor), prevented the 100 μM AMPH-elicited SRP. At 30 μM, forskolin, an activator of adenylyl cyclase, elicited SRP. The co-administration of 10 μM forskolin and 30 μM AMPH induced SRP in TS-induced reflex activity. These results implied that the repetitive/tetanic stimulation of the pelvic afferent fibers could induce SRP in PUR activities, so that the urethra can produce sufficient resistance and played a significant role in urinary retention. Findings in this study demonstrated that amphetamine could induce bladder dysfunction by triggering protein kinase A activation, and provide a practical basis for the development of treatment for amphetamine-associated urinary retention. © 2014 by The Chinese Physiological Society and Airiti Press Inc.
Original languageEnglish
Pages (from-to)171-181
Number of pages11
JournalChinese Journal of Physiology
Volume57
Issue number4
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Amphetamine
Reflex
Urine
Urinary Retention
Urethra
Colforsin
Protein Kinase Inhibitors
Cyclic AMP-Dependent Protein Kinases
Neurogenic Urinary Bladder
Dextroamphetamine
Protein C Inhibitor
Adenylyl Cyclases
Protein Kinase C
Urinary Bladder

Keywords

  • D-amphetamine
  • Micturition
  • Pelvic afferent nerve
  • Pelvic-urethra reflex
  • Reflex plasticity
  • Spinal reflex potentiation
  • cyclic AMP dependent protein kinase
  • dexamphetamine
  • forskolin
  • n [2 (4 bromocinnamylamino)ethyl] 5 isoquinolinesulfonamide
  • 2,2'-azobis(2-amidinopropane)
  • 2-amino-5-phosphopentanoic acid
  • 6 cyano 7 nitro 2,3 quinoxalinedione
  • amidine
  • amino acid receptor stimulating agent
  • benzophenanthridine derivative
  • central stimulant agent
  • chelerythrine
  • glutamic acid
  • isoquinoline derivative
  • n methylaspartic acid
  • N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide
  • oxidizing agent
  • protein kinase inhibitor
  • sulfonamide
  • valine
  • addiction
  • animal experiment
  • animal model
  • Article
  • bladder sphincter
  • controlled study
  • disease association
  • enzyme activation
  • evoked response
  • female
  • nonhuman
  • rat
  • spinal reflex
  • stimulus response
  • urine retention
  • analogs and derivatives
  • animal
  • chemically induced
  • chronic disease
  • complication
  • drug abuse
  • drug effects
  • micturition
  • pathophysiology
  • physiology
  • reflex
  • sensory nerve
  • spinal cord
  • Wistar rat
  • 6-Cyano-7-nitroquinoxaline-2,3-dione
  • Afferent Pathways
  • Amidines
  • Amphetamine-Related Disorders
  • Animals
  • Benzophenanthridines
  • Central Nervous System Stimulants
  • Chronic Disease
  • Colforsin
  • Dextroamphetamine
  • Excitatory Amino Acid Agonists
  • Female
  • Glutamic Acid
  • Isoquinolines
  • N-Methylaspartate
  • Oxidants
  • Protein Kinase Inhibitors
  • Rats, Wistar
  • Reflex
  • Spinal Cord
  • Sulfonamides
  • Urinary Retention
  • Urination
  • Valine

Cite this

Chiu, H-F., Cheng, J-K., Lin, P-H., Chang, J-C., Chen, I. M., Lin, C. I., ... Lin, C-H. (2014). A mechanistic study on urine retention in D-amphetamine addicts. Chinese Journal of Physiology, 57(4), 171-181. https://doi.org/10.4077/CJP.2014.BAC198

A mechanistic study on urine retention in D-amphetamine addicts. / Chiu, Hui-Fang; Cheng, Jen-Kun; Lin, Pu-Hua; Chang, Jung-Chen; Chen, I. Ming; Lin, Ching I.; Chang, Wei Pin; Lin, Chia-Hsien.

In: Chinese Journal of Physiology, Vol. 57, No. 4, 2014, p. 171-181.

Research output: Contribution to journalArticle

Chiu, H-F, Cheng, J-K, Lin, P-H, Chang, J-C, Chen, IM, Lin, CI, Chang, WP & Lin, C-H 2014, 'A mechanistic study on urine retention in D-amphetamine addicts', Chinese Journal of Physiology, vol. 57, no. 4, pp. 171-181. https://doi.org/10.4077/CJP.2014.BAC198
Chiu H-F, Cheng J-K, Lin P-H, Chang J-C, Chen IM, Lin CI et al. A mechanistic study on urine retention in D-amphetamine addicts. Chinese Journal of Physiology. 2014;57(4):171-181. https://doi.org/10.4077/CJP.2014.BAC198
Chiu, Hui-Fang ; Cheng, Jen-Kun ; Lin, Pu-Hua ; Chang, Jung-Chen ; Chen, I. Ming ; Lin, Ching I. ; Chang, Wei Pin ; Lin, Chia-Hsien. / A mechanistic study on urine retention in D-amphetamine addicts. In: Chinese Journal of Physiology. 2014 ; Vol. 57, No. 4. pp. 171-181.
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abstract = "Chronic amphetamine intake leads to neurogenic bladder and chronic urinary retention. The mechanism underlying persistent urinary retention is unclear. The pelvic-urethral reflex (PUR) is essential for the urethra to develop sufficient resistance to maintain urine continence, an important function of the urinary system. Recent studies on PUR activities have indicated that repetitive/tetanic stimulation of the pelvic afferent fibers induces spinal reflex potentiation (SRP) in PUR activities, which further increases urinary retention. In this study, results showed that test stimulation (TS, 1/30 Hz) evoked a baseline reflex activity, while repetitive stimulation (RS, 1 Hz) induced reflex potentiation in the external urethral sphincter. Intrathecal d-amphetamine (AMPH, 30 μM) did not but higher AMPH concentration (100 μM) induced SRP in TS-induced reflex activity. H89 (10 μM, a protein kinase A inhibitor), but not chelerythrine chloride (CTC, 10 μM, a protein kinase C inhibitor), prevented the 100 μM AMPH-elicited SRP. At 30 μM, forskolin, an activator of adenylyl cyclase, elicited SRP. The co-administration of 10 μM forskolin and 30 μM AMPH induced SRP in TS-induced reflex activity. These results implied that the repetitive/tetanic stimulation of the pelvic afferent fibers could induce SRP in PUR activities, so that the urethra can produce sufficient resistance and played a significant role in urinary retention. Findings in this study demonstrated that amphetamine could induce bladder dysfunction by triggering protein kinase A activation, and provide a practical basis for the development of treatment for amphetamine-associated urinary retention. {\circledC} 2014 by The Chinese Physiological Society and Airiti Press Inc.",
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author = "Hui-Fang Chiu and Jen-Kun Cheng and Pu-Hua Lin and Jung-Chen Chang and Chen, {I. Ming} and Lin, {Ching I.} and Chang, {Wei Pin} and Chia-Hsien Lin",
note = "Export Date: 25 March 2016 CODEN: CJPHD 通訊地址: Lin, C.-H.; Department of Health Industry Management, Kainan University, No. 1 Kainan Rd. Luzhu Shiang, Taiwan 化學物質/CAS: cyclic AMP dependent protein kinase; dexamphetamine, 1462-73-3, 51-63-8, 51-64-9; forskolin, 66575-29-9; n [2 (4 bromocinnamylamino)ethyl] 5 isoquinolinesulfonamide, 127243-85-0; 6 cyano 7 nitro 2,3 quinoxalinedione, 115066-14-3; chelerythrine, 34316-15-9; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; valine, 7004-03-7, 72-18-4; 2,2'-azobis(2-amidinopropane); 2-amino-5-phosphopentanoic acid; 6-Cyano-7-nitroquinoxaline-2,3-dione; Amidines; Benzophenanthridines; Central Nervous System Stimulants; chelerythrine; Colforsin; Dextroamphetamine; Excitatory Amino Acid Agonists; Glutamic Acid; Isoquinolines; N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide; N-Methylaspartate; Oxidants; Protein Kinase Inhibitors; Sulfonamides; Valine 參考文獻: Arnold, L.E., Kirilcuk, V., Corson, S.A., Corson, E.O.L., Levoamphetamine and dextroamphetamine: Differential effect on aggression and hyperkinesis in children and dogs (1973) Am. J. Psychiatr, 130, pp. 165-170; Beuerle, J.R., Barrueto, F., Neurogenic bladder and chronic urinary retention associated with MDMA abuse (2008) J. Med. Toxicol, 4, pp. 106-108; Chang, S., Chiang, M.J., Li, S.J., Hu, S.J., Cheng, H.Y., Hsieh, S.H., Cheng, C.L., The cooperative phenomenon of autonomic nervous system in urine storage for wistar rats (2009) Chinese J. Physiol, 52, pp. 72-80; Chang, S., Li, S.J., Hu, S.J., Cheng, H.Y., Hsieh, S.H., Cheng, C.L., Dynamic performance evaluation on the synergy of micturition in spinal cord-injured female 81-92 rats under pharmacological effects (2009) Chinese J. Physiol, 52, pp. 81-92; Chen, K.J., Chen, L.W., Liao, J.M., Chen, C.H., Ho, Y.C., Ho, Y.C., Cheng, C.L., Lin, T.B., Effects of a calcineurin inhibitor, tacrolimus, on glutamate-dependent potentiation in pelvic-urethral reflex in anesthetized rats (2006) Neuroscience, 138, pp. 69-76; Christophersen, A.S., Amphetamine designer drugs-an overview and epidemiology (2000) Toxicol. Lett, 112-113, pp. 127-131; Collingridge, G.L., Singer, W., Excitatory amino acid receptors and synaptic plasticity (1990) Trends Pharmacol. Sci, 11, pp. 290-296; de Groat, W.C., Yoshimura, N., Pharmacology of the lower urinary tract (2001) Annu. Rev. Pharmacol. Toxicol, 41, pp. 691-721; Delgado, J.H., Caruso, M.J., Waksman, J.C., Honigman, B., Stillman, D., Acute, transient urinary retention from combined ecstasy and methamphetamine use (2004) J. Emerg. Med, 26, pp. 173-175; Ellershaw, D.C., Greenwood, I.A., Large, W.A., Modulation of volume-sensitive chloride current by noradrenaline in rabbit portal vein myocytes (2002) J. Physiol, 542, pp. 537-547; Gettig, J.P., Grady, S.E., Nowosadzka, I., Methamphetamine: Putting the brakes on speed (2006) J. Sch. Nurs, 22, pp. 66-73; Hopfer, C., Mendelson, B., Van Leeuwen, J.M., Kelly, S., Hooks, S., Club drug use among youths in treatment for substance abuse (2006) Am. J. Addict, 15, pp. 94-99; Ishiura, Y., Yoshiyama, M., Yokoyama, O., Namiki, M., de Groat, W.C., Central muscarinic mechanisms regulating voiding in rats (2001) J. Pharmacol. Exp. Ther, 297, pp. 933-939; Ito, M., Long-term depression (1989) Annu. Rev. Neurosci, 12, pp. 85-102; Johnston, D., Williams, S., Jaffe, D., Gray, R., NMDA-receptorindependent long-term potentiation (1992) Annu. Rev. Physiol, 54, pp. 489-505; Kalant, H., The pharmacology and toxicology of {"}ecstasy{"} (MDMA) and related drugs (2001) Can. Med. Assoc. J, 165, pp. 917-928; Kim, M.J., Kim, E., Ryu, S.H., Suh, P.G., The mechanism of phospholipase C-gamma1 regulation (2000) Exp. Mol. Med, 32, pp. 101-109; Liao, R.M., Cheng, R.K., Acute effects of d-amphetamine on the differential reinforcement of low-rate (DRL) schedule behavior in the rat: Comparison with selective dopamine receptor antagonists (2005) Chinese J. Physiol, 48, pp. 41-50; Madison, D.V., Malenka, R.C., Nicoll, R.A., Mechanisms un derlying long-term potentiation of synaptic transmission (1991) Annu. Rev. Neurosci, 14, pp. 379-397; Martinez, J.L., Jr., Derrick, B.E., Long-term potentiation and learning (1996) Annu. Rev. Psychol, 47, pp. 173-203; Mendell, L.M., Modifiability of spinal synapses (1984) Physiol. Rev, 64, pp. 260-324; Parrott, A.C., MDMA (3,4-methylenedioxymethamphetamine) or ecstasy: The neuropsychobiological implications of taking it at dances and raves (2004) Neuropsychobiology, 50, pp. 329-335; Poikolainen, K., Ecstasy and the antecedents of illicit drug use (2006) Brit. Med. J, 332, pp. 803-804; Prinzmetal, M., Bloomberg, W., The use of bezedrine for the treatment of narcolepsy (1953) J. Am. Med. Assoc, 105, pp. 2051-2054; Randic, M., Jiang, M.C., Rusin, K.I., Cerne, R., Kolaj, M., Interactions between excitatory amino acids and tachykinins and long-term changes of synaptic responses in the rat spinal dorsal horn (1993) Regul. Pept, 46, pp. 418-420; Seamon, K.B., Daly, J.W., Forskolin: A unique diterpene activator of cyclic AMP-generating systems (1981) J. Cyclic Nucleotide Res, 7, pp. 201-224; Siegelbaum, S.A., Kandel, E.R., Learning-related synaptic plasticity: LTP and LTD (1991) Curr. Opin. Neurobiol, 1, pp. 113-120; Yaksh, T.L., Rudy, T.A., Chronic catheterization of the spinal subarachnoid space (1976) Physiol. Behav, 17, pp. 1031-1036",
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TY - JOUR

T1 - A mechanistic study on urine retention in D-amphetamine addicts

AU - Chiu, Hui-Fang

AU - Cheng, Jen-Kun

AU - Lin, Pu-Hua

AU - Chang, Jung-Chen

AU - Chen, I. Ming

AU - Lin, Ching I.

AU - Chang, Wei Pin

AU - Lin, Chia-Hsien

N1 - Export Date: 25 March 2016 CODEN: CJPHD 通訊地址: Lin, C.-H.; Department of Health Industry Management, Kainan University, No. 1 Kainan Rd. Luzhu Shiang, Taiwan 化學物質/CAS: cyclic AMP dependent protein kinase; dexamphetamine, 1462-73-3, 51-63-8, 51-64-9; forskolin, 66575-29-9; n [2 (4 bromocinnamylamino)ethyl] 5 isoquinolinesulfonamide, 127243-85-0; 6 cyano 7 nitro 2,3 quinoxalinedione, 115066-14-3; chelerythrine, 34316-15-9; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; valine, 7004-03-7, 72-18-4; 2,2'-azobis(2-amidinopropane); 2-amino-5-phosphopentanoic acid; 6-Cyano-7-nitroquinoxaline-2,3-dione; Amidines; Benzophenanthridines; Central Nervous System Stimulants; chelerythrine; Colforsin; Dextroamphetamine; Excitatory Amino Acid Agonists; Glutamic Acid; Isoquinolines; N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide; N-Methylaspartate; Oxidants; Protein Kinase Inhibitors; Sulfonamides; Valine 參考文獻: Arnold, L.E., Kirilcuk, V., Corson, S.A., Corson, E.O.L., Levoamphetamine and dextroamphetamine: Differential effect on aggression and hyperkinesis in children and dogs (1973) Am. J. Psychiatr, 130, pp. 165-170; Beuerle, J.R., Barrueto, F., Neurogenic bladder and chronic urinary retention associated with MDMA abuse (2008) J. Med. Toxicol, 4, pp. 106-108; Chang, S., Chiang, M.J., Li, S.J., Hu, S.J., Cheng, H.Y., Hsieh, S.H., Cheng, C.L., The cooperative phenomenon of autonomic nervous system in urine storage for wistar rats (2009) Chinese J. Physiol, 52, pp. 72-80; Chang, S., Li, S.J., Hu, S.J., Cheng, H.Y., Hsieh, S.H., Cheng, C.L., Dynamic performance evaluation on the synergy of micturition in spinal cord-injured female 81-92 rats under pharmacological effects (2009) Chinese J. Physiol, 52, pp. 81-92; Chen, K.J., Chen, L.W., Liao, J.M., Chen, C.H., Ho, Y.C., Ho, Y.C., Cheng, C.L., Lin, T.B., Effects of a calcineurin inhibitor, tacrolimus, on glutamate-dependent potentiation in pelvic-urethral reflex in anesthetized rats (2006) Neuroscience, 138, pp. 69-76; Christophersen, A.S., Amphetamine designer drugs-an overview and epidemiology (2000) Toxicol. Lett, 112-113, pp. 127-131; Collingridge, G.L., Singer, W., Excitatory amino acid receptors and synaptic plasticity (1990) Trends Pharmacol. Sci, 11, pp. 290-296; de Groat, W.C., Yoshimura, N., Pharmacology of the lower urinary tract (2001) Annu. Rev. Pharmacol. Toxicol, 41, pp. 691-721; Delgado, J.H., Caruso, M.J., Waksman, J.C., Honigman, B., Stillman, D., Acute, transient urinary retention from combined ecstasy and methamphetamine use (2004) J. Emerg. Med, 26, pp. 173-175; Ellershaw, D.C., Greenwood, I.A., Large, W.A., Modulation of volume-sensitive chloride current by noradrenaline in rabbit portal vein myocytes (2002) J. Physiol, 542, pp. 537-547; Gettig, J.P., Grady, S.E., Nowosadzka, I., Methamphetamine: Putting the brakes on speed (2006) J. Sch. Nurs, 22, pp. 66-73; Hopfer, C., Mendelson, B., Van Leeuwen, J.M., Kelly, S., Hooks, S., Club drug use among youths in treatment for substance abuse (2006) Am. J. Addict, 15, pp. 94-99; Ishiura, Y., Yoshiyama, M., Yokoyama, O., Namiki, M., de Groat, W.C., Central muscarinic mechanisms regulating voiding in rats (2001) J. Pharmacol. Exp. Ther, 297, pp. 933-939; Ito, M., Long-term depression (1989) Annu. Rev. Neurosci, 12, pp. 85-102; Johnston, D., Williams, S., Jaffe, D., Gray, R., NMDA-receptorindependent long-term potentiation (1992) Annu. Rev. Physiol, 54, pp. 489-505; Kalant, H., The pharmacology and toxicology of "ecstasy" (MDMA) and related drugs (2001) Can. Med. Assoc. J, 165, pp. 917-928; Kim, M.J., Kim, E., Ryu, S.H., Suh, P.G., The mechanism of phospholipase C-gamma1 regulation (2000) Exp. Mol. Med, 32, pp. 101-109; Liao, R.M., Cheng, R.K., Acute effects of d-amphetamine on the differential reinforcement of low-rate (DRL) schedule behavior in the rat: Comparison with selective dopamine receptor antagonists (2005) Chinese J. Physiol, 48, pp. 41-50; Madison, D.V., Malenka, R.C., Nicoll, R.A., Mechanisms un derlying long-term potentiation of synaptic transmission (1991) Annu. Rev. Neurosci, 14, pp. 379-397; Martinez, J.L., Jr., Derrick, B.E., Long-term potentiation and learning (1996) Annu. Rev. Psychol, 47, pp. 173-203; Mendell, L.M., Modifiability of spinal synapses (1984) Physiol. Rev, 64, pp. 260-324; Parrott, A.C., MDMA (3,4-methylenedioxymethamphetamine) or ecstasy: The neuropsychobiological implications of taking it at dances and raves (2004) Neuropsychobiology, 50, pp. 329-335; Poikolainen, K., Ecstasy and the antecedents of illicit drug use (2006) Brit. Med. J, 332, pp. 803-804; Prinzmetal, M., Bloomberg, W., The use of bezedrine for the treatment of narcolepsy (1953) J. Am. Med. Assoc, 105, pp. 2051-2054; Randic, M., Jiang, M.C., Rusin, K.I., Cerne, R., Kolaj, M., Interactions between excitatory amino acids and tachykinins and long-term changes of synaptic responses in the rat spinal dorsal horn (1993) Regul. Pept, 46, pp. 418-420; Seamon, K.B., Daly, J.W., Forskolin: A unique diterpene activator of cyclic AMP-generating systems (1981) J. Cyclic Nucleotide Res, 7, pp. 201-224; Siegelbaum, S.A., Kandel, E.R., Learning-related synaptic plasticity: LTP and LTD (1991) Curr. Opin. Neurobiol, 1, pp. 113-120; Yaksh, T.L., Rudy, T.A., Chronic catheterization of the spinal subarachnoid space (1976) Physiol. Behav, 17, pp. 1031-1036

PY - 2014

Y1 - 2014

N2 - Chronic amphetamine intake leads to neurogenic bladder and chronic urinary retention. The mechanism underlying persistent urinary retention is unclear. The pelvic-urethral reflex (PUR) is essential for the urethra to develop sufficient resistance to maintain urine continence, an important function of the urinary system. Recent studies on PUR activities have indicated that repetitive/tetanic stimulation of the pelvic afferent fibers induces spinal reflex potentiation (SRP) in PUR activities, which further increases urinary retention. In this study, results showed that test stimulation (TS, 1/30 Hz) evoked a baseline reflex activity, while repetitive stimulation (RS, 1 Hz) induced reflex potentiation in the external urethral sphincter. Intrathecal d-amphetamine (AMPH, 30 μM) did not but higher AMPH concentration (100 μM) induced SRP in TS-induced reflex activity. H89 (10 μM, a protein kinase A inhibitor), but not chelerythrine chloride (CTC, 10 μM, a protein kinase C inhibitor), prevented the 100 μM AMPH-elicited SRP. At 30 μM, forskolin, an activator of adenylyl cyclase, elicited SRP. The co-administration of 10 μM forskolin and 30 μM AMPH induced SRP in TS-induced reflex activity. These results implied that the repetitive/tetanic stimulation of the pelvic afferent fibers could induce SRP in PUR activities, so that the urethra can produce sufficient resistance and played a significant role in urinary retention. Findings in this study demonstrated that amphetamine could induce bladder dysfunction by triggering protein kinase A activation, and provide a practical basis for the development of treatment for amphetamine-associated urinary retention. © 2014 by The Chinese Physiological Society and Airiti Press Inc.

AB - Chronic amphetamine intake leads to neurogenic bladder and chronic urinary retention. The mechanism underlying persistent urinary retention is unclear. The pelvic-urethral reflex (PUR) is essential for the urethra to develop sufficient resistance to maintain urine continence, an important function of the urinary system. Recent studies on PUR activities have indicated that repetitive/tetanic stimulation of the pelvic afferent fibers induces spinal reflex potentiation (SRP) in PUR activities, which further increases urinary retention. In this study, results showed that test stimulation (TS, 1/30 Hz) evoked a baseline reflex activity, while repetitive stimulation (RS, 1 Hz) induced reflex potentiation in the external urethral sphincter. Intrathecal d-amphetamine (AMPH, 30 μM) did not but higher AMPH concentration (100 μM) induced SRP in TS-induced reflex activity. H89 (10 μM, a protein kinase A inhibitor), but not chelerythrine chloride (CTC, 10 μM, a protein kinase C inhibitor), prevented the 100 μM AMPH-elicited SRP. At 30 μM, forskolin, an activator of adenylyl cyclase, elicited SRP. The co-administration of 10 μM forskolin and 30 μM AMPH induced SRP in TS-induced reflex activity. These results implied that the repetitive/tetanic stimulation of the pelvic afferent fibers could induce SRP in PUR activities, so that the urethra can produce sufficient resistance and played a significant role in urinary retention. Findings in this study demonstrated that amphetamine could induce bladder dysfunction by triggering protein kinase A activation, and provide a practical basis for the development of treatment for amphetamine-associated urinary retention. © 2014 by The Chinese Physiological Society and Airiti Press Inc.

KW - D-amphetamine

KW - Micturition

KW - Pelvic afferent nerve

KW - Pelvic-urethra reflex

KW - Reflex plasticity

KW - Spinal reflex potentiation

KW - cyclic AMP dependent protein kinase

KW - dexamphetamine

KW - forskolin

KW - n [2 (4 bromocinnamylamino)ethyl] 5 isoquinolinesulfonamide

KW - 2,2'-azobis(2-amidinopropane)

KW - 2-amino-5-phosphopentanoic acid

KW - 6 cyano 7 nitro 2,3 quinoxalinedione

KW - amidine

KW - amino acid receptor stimulating agent

KW - benzophenanthridine derivative

KW - central stimulant agent

KW - chelerythrine

KW - glutamic acid

KW - isoquinoline derivative

KW - n methylaspartic acid

KW - N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide

KW - oxidizing agent

KW - protein kinase inhibitor

KW - sulfonamide

KW - valine

KW - addiction

KW - animal experiment

KW - animal model

KW - Article

KW - bladder sphincter

KW - controlled study

KW - disease association

KW - enzyme activation

KW - evoked response

KW - female

KW - nonhuman

KW - rat

KW - spinal reflex

KW - stimulus response

KW - urine retention

KW - analogs and derivatives

KW - animal

KW - chemically induced

KW - chronic disease

KW - complication

KW - drug abuse

KW - drug effects

KW - micturition

KW - pathophysiology

KW - physiology

KW - reflex

KW - sensory nerve

KW - spinal cord

KW - Wistar rat

KW - 6-Cyano-7-nitroquinoxaline-2,3-dione

KW - Afferent Pathways

KW - Amidines

KW - Amphetamine-Related Disorders

KW - Animals

KW - Benzophenanthridines

KW - Central Nervous System Stimulants

KW - Chronic Disease

KW - Colforsin

KW - Dextroamphetamine

KW - Excitatory Amino Acid Agonists

KW - Female

KW - Glutamic Acid

KW - Isoquinolines

KW - N-Methylaspartate

KW - Oxidants

KW - Protein Kinase Inhibitors

KW - Rats, Wistar

KW - Reflex

KW - Spinal Cord

KW - Sulfonamides

KW - Urinary Retention

KW - Urination

KW - Valine

U2 - 10.4077/CJP.2014.BAC198

DO - 10.4077/CJP.2014.BAC198

M3 - Article

VL - 57

SP - 171

EP - 181

JO - Chinese Journal of Physiology

JF - Chinese Journal of Physiology

SN - 0304-4920

IS - 4

ER -