A feasible tool to detect mRNA expression of matrix metalloproteinases and their tissue inhibitors in human Tenon's capsule

Yu Lun Huang, Catherine J. Liu, Allen W. Chiu, Yu Chun Wang, Steven K. Huan, Fenq Lih Lee, Shih Jen Chen, Wen-Ming Hsu, Shie Liang Hsieh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose: To determine the mRNA expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in human specimens of Tenon's capsule. Methods: Reverse transcription-polymerase chain reaction (RT-PCR) with specific primers for MMP-1, MMP-2, MMP-9, TIMP-1 and TIMP-2 was performed on tissue specimens obtained from patients with cataract, rhegmatogenous retinal detachment, glaucoma, or diabetes mellitus. Results: Glyceraldehyde phosphate dehydrogenase (GAPDH) mRNA transcripts were detected in 26 (76.5%) of 34 specimens, with almost the same amount of expression in each of these samples. Messenger RNA expression of one or more of the MMPs/TIMPs could also be detected in all of these 26 samples, but not in any sample without GAPDH expression. MMP-2, TIMP-1 and TIMP-2 were detected in 25 (96.2%) of the 26 samples with GAPDH expression, while MMP-1 and MMP-9 were detected with a lower percentage (34.6 and 19.2%, respectively). Conclusions: The feasibility of RT-PCR with GAPDH as an internal standard to determine mRNA transcripts of the MMPs and TIMPs in the subcon-junctival Tenon's capsule was demonstrated.

Original languageEnglish
Pages (from-to)375-379
Number of pages5
JournalOphthalmic Research
Volume34
Issue number6
DOIs
Publication statusPublished - Dec 1 2002
Externally publishedYes

Fingerprint

Tenon Capsule
Glyceraldehyde
Matrix Metalloproteinases
Tissue Inhibitor of Metalloproteinases
Matrix Metalloproteinase Inhibitors
Oxidoreductases
Phosphates
Tissue Inhibitor of Metalloproteinase-2
Messenger RNA
Matrix Metalloproteinase 1
Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Reverse Transcription
Polymerase Chain Reaction
Retinal Detachment
Glaucoma
Cataract
Diabetes Mellitus

Keywords

  • Eye
  • Matrix metalloproteinase
  • Reverse transcription-polymerase chain reaction
  • Tenon's capsule
  • Tissue inhibitor of metalloproteinase

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Huang, Y. L., Liu, C. J., Chiu, A. W., Wang, Y. C., Huan, S. K., Lee, F. L., ... Hsieh, S. L. (2002). A feasible tool to detect mRNA expression of matrix metalloproteinases and their tissue inhibitors in human Tenon's capsule. Ophthalmic Research, 34(6), 375-379. https://doi.org/10.1159/000067044

A feasible tool to detect mRNA expression of matrix metalloproteinases and their tissue inhibitors in human Tenon's capsule. / Huang, Yu Lun; Liu, Catherine J.; Chiu, Allen W.; Wang, Yu Chun; Huan, Steven K.; Lee, Fenq Lih; Chen, Shih Jen; Hsu, Wen-Ming; Hsieh, Shie Liang.

In: Ophthalmic Research, Vol. 34, No. 6, 01.12.2002, p. 375-379.

Research output: Contribution to journalArticle

Huang, Yu Lun ; Liu, Catherine J. ; Chiu, Allen W. ; Wang, Yu Chun ; Huan, Steven K. ; Lee, Fenq Lih ; Chen, Shih Jen ; Hsu, Wen-Ming ; Hsieh, Shie Liang. / A feasible tool to detect mRNA expression of matrix metalloproteinases and their tissue inhibitors in human Tenon's capsule. In: Ophthalmic Research. 2002 ; Vol. 34, No. 6. pp. 375-379.
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