Abstract
Purpose: To determine the mRNA expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in human specimens of Tenon's capsule. Methods: Reverse transcription-polymerase chain reaction (RT-PCR) with specific primers for MMP-1, MMP-2, MMP-9, TIMP-1 and TIMP-2 was performed on tissue specimens obtained from patients with cataract, rhegmatogenous retinal detachment, glaucoma, or diabetes mellitus. Results: Glyceraldehyde phosphate dehydrogenase (GAPDH) mRNA transcripts were detected in 26 (76.5%) of 34 specimens, with almost the same amount of expression in each of these samples. Messenger RNA expression of one or more of the MMPs/TIMPs could also be detected in all of these 26 samples, but not in any sample without GAPDH expression. MMP-2, TIMP-1 and TIMP-2 were detected in 25 (96.2%) of the 26 samples with GAPDH expression, while MMP-1 and MMP-9 were detected with a lower percentage (34.6 and 19.2%, respectively). Conclusions: The feasibility of RT-PCR with GAPDH as an internal standard to determine mRNA transcripts of the MMPs and TIMPs in the subcon-junctival Tenon's capsule was demonstrated.
| Original language | English |
|---|---|
| Pages (from-to) | 375-379 |
| Number of pages | 5 |
| Journal | Ophthalmic Research |
| Volume | 34 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 1 2002 |
| Externally published | Yes |
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Keywords
- Eye
- Matrix metalloproteinase
- Reverse transcription-polymerase chain reaction
- Tenon's capsule
- Tissue inhibitor of metalloproteinase
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience
Cite this
A feasible tool to detect mRNA expression of matrix metalloproteinases and their tissue inhibitors in human Tenon's capsule. / Huang, Yu Lun; Liu, Catherine J.; Chiu, Allen W.; Wang, Yu Chun; Huan, Steven K.; Lee, Fenq Lih; Chen, Shih Jen; Hsu, Wen-Ming; Hsieh, Shie Liang.
In: Ophthalmic Research, Vol. 34, No. 6, 01.12.2002, p. 375-379.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A feasible tool to detect mRNA expression of matrix metalloproteinases and their tissue inhibitors in human Tenon's capsule
AU - Huang, Yu Lun
AU - Liu, Catherine J.
AU - Chiu, Allen W.
AU - Wang, Yu Chun
AU - Huan, Steven K.
AU - Lee, Fenq Lih
AU - Chen, Shih Jen
AU - Hsu, Wen-Ming
AU - Hsieh, Shie Liang
PY - 2002/12/1
Y1 - 2002/12/1
N2 - Purpose: To determine the mRNA expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in human specimens of Tenon's capsule. Methods: Reverse transcription-polymerase chain reaction (RT-PCR) with specific primers for MMP-1, MMP-2, MMP-9, TIMP-1 and TIMP-2 was performed on tissue specimens obtained from patients with cataract, rhegmatogenous retinal detachment, glaucoma, or diabetes mellitus. Results: Glyceraldehyde phosphate dehydrogenase (GAPDH) mRNA transcripts were detected in 26 (76.5%) of 34 specimens, with almost the same amount of expression in each of these samples. Messenger RNA expression of one or more of the MMPs/TIMPs could also be detected in all of these 26 samples, but not in any sample without GAPDH expression. MMP-2, TIMP-1 and TIMP-2 were detected in 25 (96.2%) of the 26 samples with GAPDH expression, while MMP-1 and MMP-9 were detected with a lower percentage (34.6 and 19.2%, respectively). Conclusions: The feasibility of RT-PCR with GAPDH as an internal standard to determine mRNA transcripts of the MMPs and TIMPs in the subcon-junctival Tenon's capsule was demonstrated.
AB - Purpose: To determine the mRNA expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in human specimens of Tenon's capsule. Methods: Reverse transcription-polymerase chain reaction (RT-PCR) with specific primers for MMP-1, MMP-2, MMP-9, TIMP-1 and TIMP-2 was performed on tissue specimens obtained from patients with cataract, rhegmatogenous retinal detachment, glaucoma, or diabetes mellitus. Results: Glyceraldehyde phosphate dehydrogenase (GAPDH) mRNA transcripts were detected in 26 (76.5%) of 34 specimens, with almost the same amount of expression in each of these samples. Messenger RNA expression of one or more of the MMPs/TIMPs could also be detected in all of these 26 samples, but not in any sample without GAPDH expression. MMP-2, TIMP-1 and TIMP-2 were detected in 25 (96.2%) of the 26 samples with GAPDH expression, while MMP-1 and MMP-9 were detected with a lower percentage (34.6 and 19.2%, respectively). Conclusions: The feasibility of RT-PCR with GAPDH as an internal standard to determine mRNA transcripts of the MMPs and TIMPs in the subcon-junctival Tenon's capsule was demonstrated.
KW - Eye
KW - Matrix metalloproteinase
KW - Reverse transcription-polymerase chain reaction
KW - Tenon's capsule
KW - Tissue inhibitor of metalloproteinase
UR - http://www.scopus.com/inward/record.url?scp=0036974462&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036974462&partnerID=8YFLogxK
U2 - 10.1159/000067044
DO - 10.1159/000067044
M3 - Article
C2 - 12483026
AN - SCOPUS:0036974462
VL - 34
SP - 375
EP - 379
JO - Ophthalmic Research
JF - Ophthalmic Research
SN - 0030-3747
IS - 6
ER -