A facile measurement for monitoring dragline silk dope concentration in Nephila pilipes upon spinning

Hsuan Chen Wu, Shang Ru Wu, Thomas Chung Kuang Yang, Jen Chang Yang

Research output: Contribution to journalArticle

Abstract

In spite of all the efforts towards deciphering the silk spinning process of spiders, the underlying mechanism is yet to be fully revealed. In this research, we designed a novel approach that allowed us to quantitatively evaluate the concentration change of silk dope during the liquid-to-solid spinning process of the orb-weaver Nephila pilipes. As a prior characterization of the optimal silking conditions, we first gauged the influence of silking-rate, ranging from 1.5 to 8.0 m/min, on dragline silk diameters and silk tensile strengths obtained from the spiders. Next, to evaluate the liquid content of the silk dope, the major ampullate gland was dissected and the concentration of the sac portion was measured by thermogravimetric analysis (TGA). The solid content of the dragline fibers leaving the spinneret was investigated by calculating the ratio of collected dried silk to the weight loss of the spider recorded in situ upon spinning. As the results indicate, the tensile strength and diameter of the spun dragline fibers were 800-1100 MPa and 8-11 μm, respectively. The liquid content of silk stored in the major ampullate sac (50.0 wt%) was significantly lower than that of silk leaving the spinnerets (80.9-96.1 wt%), indicating that a liquid supplying mechanism might be involved during the spinning process. This reveals, for the first time, quantitative evidence in support of the lubricative hypothesis proposed formerly, namely that a liquid coating layer is supplemented to compensate for silking resistance during the spinning process of a spider. The spigot, at the exit of the spinneret, is speculated to serve as a valve-like controller that regulates the lubrication process along with fiber formation. Taken together, these findings provide understanding of the physiological functions in the spider spinning process and could further shed some light on the future biomimetic development of silk material fabrication.

Original languageEnglish
Article number1951
JournalMaterials
Volume11
Issue number10
DOIs
Publication statusPublished - Oct 12 2018

Fingerprint

Silk
Monitoring
Liquids
Fibers
Tensile strength
Biomimetics
Lubrication
Thermogravimetric analysis
Fabrication
Coatings
Controllers

Keywords

  • Major ampullate gland
  • Nephila pilipes
  • Silk
  • Spider

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

A facile measurement for monitoring dragline silk dope concentration in Nephila pilipes upon spinning. / Wu, Hsuan Chen; Wu, Shang Ru; Yang, Thomas Chung Kuang; Yang, Jen Chang.

In: Materials, Vol. 11, No. 10, 1951, 12.10.2018.

Research output: Contribution to journalArticle

Wu, Hsuan Chen ; Wu, Shang Ru ; Yang, Thomas Chung Kuang ; Yang, Jen Chang. / A facile measurement for monitoring dragline silk dope concentration in Nephila pilipes upon spinning. In: Materials. 2018 ; Vol. 11, No. 10.
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abstract = "In spite of all the efforts towards deciphering the silk spinning process of spiders, the underlying mechanism is yet to be fully revealed. In this research, we designed a novel approach that allowed us to quantitatively evaluate the concentration change of silk dope during the liquid-to-solid spinning process of the orb-weaver Nephila pilipes. As a prior characterization of the optimal silking conditions, we first gauged the influence of silking-rate, ranging from 1.5 to 8.0 m/min, on dragline silk diameters and silk tensile strengths obtained from the spiders. Next, to evaluate the liquid content of the silk dope, the major ampullate gland was dissected and the concentration of the sac portion was measured by thermogravimetric analysis (TGA). The solid content of the dragline fibers leaving the spinneret was investigated by calculating the ratio of collected dried silk to the weight loss of the spider recorded in situ upon spinning. As the results indicate, the tensile strength and diameter of the spun dragline fibers were 800-1100 MPa and 8-11 μm, respectively. The liquid content of silk stored in the major ampullate sac (50.0 wt{\%}) was significantly lower than that of silk leaving the spinnerets (80.9-96.1 wt{\%}), indicating that a liquid supplying mechanism might be involved during the spinning process. This reveals, for the first time, quantitative evidence in support of the lubricative hypothesis proposed formerly, namely that a liquid coating layer is supplemented to compensate for silking resistance during the spinning process of a spider. The spigot, at the exit of the spinneret, is speculated to serve as a valve-like controller that regulates the lubrication process along with fiber formation. Taken together, these findings provide understanding of the physiological functions in the spider spinning process and could further shed some light on the future biomimetic development of silk material fabrication.",
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