A CTLA-4 Antagonizing DNA Aptamer with Antitumor Effect

Bo Tsang Huang, Wei Yun Lai, Yi Chung Chang, Jen Wei Wang, Shauh Der Yeh, Emily Pei Ying Lin, Pan Chyr Yang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The successful translation of cytotoxic T lymphocyte antigen-4 (CTLA-4) blockade has revolutionized the concept of cancer immunotherapy. Although monoclonal antibody therapeutics remain the mainstream in clinical practice, aptamers are synthetic oligonucleotides that encompass antibody-mimicking functions. Here, we report a novel high-affinity CTLA-4-antagonizing DNA aptamer (dissociation constant, 11.84 nM), aptCTLA-4, which was identified by cell-based SELEX and high-throughput sequencing. aptCTLA-4 is relatively stable in serum, promotes lymphocyte proliferation, and inhibits tumor growth in cell and animal models. Our study demonstrates the developmental pipeline of a functional CTLA-4-targeting aptamer and suggests a translational potential for aptCTLA-4.

Original languageEnglish
Pages (from-to)520-528
Number of pages9
JournalMolecular Therapy - Nucleic Acids
Volume8
DOIs
Publication statusPublished - Sep 15 2017

Fingerprint

CTLA-4 Antigen
Nucleotide Aptamers
Oligonucleotides
Immunotherapy
Neoplasms
Animal Models
Monoclonal Antibodies
Lymphocytes
Antibodies
Growth
Serum
Therapeutics

Keywords

  • aptamer
  • cancer
  • CTLA-4
  • immunotherapy

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery

Cite this

Huang, B. T., Lai, W. Y., Chang, Y. C., Wang, J. W., Yeh, S. D., Lin, E. P. Y., & Yang, P. C. (2017). A CTLA-4 Antagonizing DNA Aptamer with Antitumor Effect. Molecular Therapy - Nucleic Acids, 8, 520-528. https://doi.org/10.1016/j.omtn.2017.08.006

A CTLA-4 Antagonizing DNA Aptamer with Antitumor Effect. / Huang, Bo Tsang; Lai, Wei Yun; Chang, Yi Chung; Wang, Jen Wei; Yeh, Shauh Der; Lin, Emily Pei Ying; Yang, Pan Chyr.

In: Molecular Therapy - Nucleic Acids, Vol. 8, 15.09.2017, p. 520-528.

Research output: Contribution to journalArticle

Huang, BT, Lai, WY, Chang, YC, Wang, JW, Yeh, SD, Lin, EPY & Yang, PC 2017, 'A CTLA-4 Antagonizing DNA Aptamer with Antitumor Effect', Molecular Therapy - Nucleic Acids, vol. 8, pp. 520-528. https://doi.org/10.1016/j.omtn.2017.08.006
Huang, Bo Tsang ; Lai, Wei Yun ; Chang, Yi Chung ; Wang, Jen Wei ; Yeh, Shauh Der ; Lin, Emily Pei Ying ; Yang, Pan Chyr. / A CTLA-4 Antagonizing DNA Aptamer with Antitumor Effect. In: Molecular Therapy - Nucleic Acids. 2017 ; Vol. 8. pp. 520-528.
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