A crystallographic investigation of citrate synthase from pig and chicken heart muscle

Andrew H.J. Wang, M. L. Sherman, Alexander Rich

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Citrate synthase (EC 4. 1. 3. 7.) from pig heart and chicken heart muscle can be crystallized from 10 mM phosphate buffer (pH 7. 0-7. 5) and 10-15% PEG4000 solution. The space group is P41212 with one subunit/asymmetric unit for the pig heart enzyme. The chicken heart citrate synthase purified from Blue-dextran as well as ATP-Sepharose affinity chromatography both crystallize in space group P21212 with one molecule/asymmetric unit, but they have different unit cell dimensions. The a-axis differs by about 17 Å between these two crystal forms, while b- and c-axis dimensions are virtually identical.

Original languageEnglish
Pages (from-to)150-156
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume82
Issue number1
DOIs
Publication statusPublished - May 15 1978
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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