A comprehensive analysis of recruitment and transactivation potential of K-Rta and K-bZIP during reactivation of Kaposi's sarcoma-associated herpesvirus

Thomas J. Ellison, Yoshihiro Izumiya, Chie Izumiya, Paul A. Luciw, Hsing Jien Kung

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent of Kaposi's sarcoma. K-Rta and K-bZIP are two major viral transcription factors that control reactivation of this virus. Here we report a genome-wide analysis of transcriptional capacity by evaluation of a comprehensive library of 83 putative KSHV promoters. In reporter assays, 34 viral promoters were activated by K-Rta, whereas K-bZIP activated 21 promoters. When K-Rta and K-bZIP were combined, 3 K-Rta responsive promoters were repressed by K-bZIP. The occupancy of K-Rta and K-bZIP across KSHV promoters was analyzed by chromatin immunoprecipitation with a viral promoter-chip in BCBL-1 cells. In addition, acetylation of local histones was examined to determine accessibility of promoters during latency and reactivation. Finally, 10 promoters were selected to study the dynamics of transcription factor recruitment. This study provides a comprehensive overview of the responsiveness of KSHV promoters to K-Rta and K-bZIP, and describes key chromatin changes during viral reactivation.

Original languageEnglish
Pages (from-to)76-88
Number of pages13
JournalVirology
Volume387
Issue number1
DOIs
Publication statusPublished - Apr 25 2009
Externally publishedYes

Fingerprint

Human Herpesvirus 8
Transcriptional Activation
Transcription Factors
Chromatin Immunoprecipitation
Kaposi's Sarcoma
Acetylation
Histones
Chromatin
Genome
Viruses

Keywords

  • Chromatin
  • Chromatin immunoprecipitation
  • Gene expression
  • HHV-8
  • K-bZIP/K8
  • K-Rta/ORF50
  • Kaposi's sarcoma
  • KSHV
  • Microarray
  • Transcription

ASJC Scopus subject areas

  • Virology

Cite this

A comprehensive analysis of recruitment and transactivation potential of K-Rta and K-bZIP during reactivation of Kaposi's sarcoma-associated herpesvirus. / Ellison, Thomas J.; Izumiya, Yoshihiro; Izumiya, Chie; Luciw, Paul A.; Kung, Hsing Jien.

In: Virology, Vol. 387, No. 1, 25.04.2009, p. 76-88.

Research output: Contribution to journalArticle

@article{32d6f1341de34d0891733358e6d249fd,
title = "A comprehensive analysis of recruitment and transactivation potential of K-Rta and K-bZIP during reactivation of Kaposi's sarcoma-associated herpesvirus",
abstract = "Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent of Kaposi's sarcoma. K-Rta and K-bZIP are two major viral transcription factors that control reactivation of this virus. Here we report a genome-wide analysis of transcriptional capacity by evaluation of a comprehensive library of 83 putative KSHV promoters. In reporter assays, 34 viral promoters were activated by K-Rta, whereas K-bZIP activated 21 promoters. When K-Rta and K-bZIP were combined, 3 K-Rta responsive promoters were repressed by K-bZIP. The occupancy of K-Rta and K-bZIP across KSHV promoters was analyzed by chromatin immunoprecipitation with a viral promoter-chip in BCBL-1 cells. In addition, acetylation of local histones was examined to determine accessibility of promoters during latency and reactivation. Finally, 10 promoters were selected to study the dynamics of transcription factor recruitment. This study provides a comprehensive overview of the responsiveness of KSHV promoters to K-Rta and K-bZIP, and describes key chromatin changes during viral reactivation.",
keywords = "Chromatin, Chromatin immunoprecipitation, Gene expression, HHV-8, K-bZIP/K8, K-Rta/ORF50, Kaposi's sarcoma, KSHV, Microarray, Transcription",
author = "Ellison, {Thomas J.} and Yoshihiro Izumiya and Chie Izumiya and Luciw, {Paul A.} and Kung, {Hsing Jien}",
year = "2009",
month = "4",
day = "25",
doi = "10.1016/j.virol.2009.02.016",
language = "English",
volume = "387",
pages = "76--88",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - A comprehensive analysis of recruitment and transactivation potential of K-Rta and K-bZIP during reactivation of Kaposi's sarcoma-associated herpesvirus

AU - Ellison, Thomas J.

AU - Izumiya, Yoshihiro

AU - Izumiya, Chie

AU - Luciw, Paul A.

AU - Kung, Hsing Jien

PY - 2009/4/25

Y1 - 2009/4/25

N2 - Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent of Kaposi's sarcoma. K-Rta and K-bZIP are two major viral transcription factors that control reactivation of this virus. Here we report a genome-wide analysis of transcriptional capacity by evaluation of a comprehensive library of 83 putative KSHV promoters. In reporter assays, 34 viral promoters were activated by K-Rta, whereas K-bZIP activated 21 promoters. When K-Rta and K-bZIP were combined, 3 K-Rta responsive promoters were repressed by K-bZIP. The occupancy of K-Rta and K-bZIP across KSHV promoters was analyzed by chromatin immunoprecipitation with a viral promoter-chip in BCBL-1 cells. In addition, acetylation of local histones was examined to determine accessibility of promoters during latency and reactivation. Finally, 10 promoters were selected to study the dynamics of transcription factor recruitment. This study provides a comprehensive overview of the responsiveness of KSHV promoters to K-Rta and K-bZIP, and describes key chromatin changes during viral reactivation.

AB - Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent of Kaposi's sarcoma. K-Rta and K-bZIP are two major viral transcription factors that control reactivation of this virus. Here we report a genome-wide analysis of transcriptional capacity by evaluation of a comprehensive library of 83 putative KSHV promoters. In reporter assays, 34 viral promoters were activated by K-Rta, whereas K-bZIP activated 21 promoters. When K-Rta and K-bZIP were combined, 3 K-Rta responsive promoters were repressed by K-bZIP. The occupancy of K-Rta and K-bZIP across KSHV promoters was analyzed by chromatin immunoprecipitation with a viral promoter-chip in BCBL-1 cells. In addition, acetylation of local histones was examined to determine accessibility of promoters during latency and reactivation. Finally, 10 promoters were selected to study the dynamics of transcription factor recruitment. This study provides a comprehensive overview of the responsiveness of KSHV promoters to K-Rta and K-bZIP, and describes key chromatin changes during viral reactivation.

KW - Chromatin

KW - Chromatin immunoprecipitation

KW - Gene expression

KW - HHV-8

KW - K-bZIP/K8

KW - K-Rta/ORF50

KW - Kaposi's sarcoma

KW - KSHV

KW - Microarray

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=63549108515&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=63549108515&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2009.02.016

DO - 10.1016/j.virol.2009.02.016

M3 - Article

C2 - 19269659

AN - SCOPUS:63549108515

VL - 387

SP - 76

EP - 88

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -