A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways

Yi Chung Chien, Ming Jyh Sheu, Chieh Hsi Wu, Wen Hsin Lin, Ying Yi Chen, Po Liang Cheng, Hsu Chen Cheng

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.Methods: VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.Results: Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.Conclusions: These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

Original languageEnglish
Article number137
JournalBMC Complementary and Alternative Medicine
Volume12
DOIs
Publication statusPublished - Aug 24 2012
Externally publishedYes

Keywords

  • Chinese herbs
  • Gan-Lu-Yin
  • Migration
  • Restenosis
  • Vascular smooth muscle cell

ASJC Scopus subject areas

  • Complementary and alternative medicine

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