A chemically functionalized magnetic nanoplatform for rapid and specific biomolecular recognition and separation

Po Chiao Lin, Ching Ching Yu, Huan Ting Wu, Ying Wei Lu, Chia Li Han, An Kai Su, Yu Ju Chen, Chun Cheng Lin

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have developed a target-molecule-functionalized magnetic nanoparticle (MNP)-based method to facilitate the study of biomolecular recognition and separation. The superparamagnetic property of MNPs allows the corresponding biomolecules to be rapidly separated from crude biofluids with a significant improvement in recovery yield and specificity. Various MNPs functionalized with tag molecules (chitin, heparin, and amylose) were synthesized for recombinant protein purification, and several probe-functionalized MNPs, such as nitrilotriacetic acid (NTA)@MNP and Pk@MNP, exhibited excellent extraction efficiency for proteins. In a cell recognition study, mannose-functionalized MNPs allowed specific purification of Escherichia coli with FimH adhesin on the surface. In an immunoprecipitation assay, the antibody-conjugated MNPs reduced the incubation time from 12 to 1 h while maintaining a comparable efficiency. The functionalized MNPs were also used in a membrane proteomic study that utilized the interaction between streptavidin-functionalized MNPs and biotinylated cell membrane proteins. Overall, the functionalized MNPs were demonstrated to be promising probes for the specific separation of targets from proteins to cells and proteomics.

Original languageEnglish
Pages (from-to)160-168
Number of pages9
JournalBiomacromolecules
Volume14
Issue number1
DOIs
Publication statusPublished - Jan 14 2013
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials

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