A cell line (EP-1 cell line) derived from "Beko disease" affected Japanese eel elver (Anguilla japonica) persistently infected with Pleistophora anguillarum

Guang Hsiung Kou, Chung Hsiung Wang, Hsu Wei Hung, Yun Shiang Jang, Chih Ming Chou, Chu Fang Lo

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Tissues collected from Japanese eel elver (Anguilla japonica) experimentally infected with Pleistophora anguillarum were cultivated in Leiboritz's L-15 medium. A cell line designated as EP-1 (eel cells persistently infected with P. anguillarum) was established, and was found to be persistently infected with P. anguillarum. With this culture system, we first described the merogonial reproduction of P. anguillarum. Using histochemical and indirect immunofluorescent technique, it was revealed that there were 3-4 merozoites of different sizes residing in the cytoplasm of EP-1 cells. Electron micrographs of EP-1 cells showed that the merogonial stages had isolated nuclei (monokaryotic). The merozoite plasmalemma was separated from host cytoplasm by a membrane of host origin. The results of the infection experiment revealed that the present in vitro cultivation system could provide infective merozoites which were capable of proceeding to further sporogonial reproduction in the muscle of their natural host, eels.

Original languageEnglish
Pages (from-to)161-173
Number of pages13
JournalAquaculture
Volume132
Issue number1-2
DOIs
Publication statusPublished - Apr 15 1995
Externally publishedYes

    Fingerprint

Keywords

  • Anguilla japonica
  • Beko disease of eel
  • Microspora
  • Pleistophora anguillarum

ASJC Scopus subject areas

  • Aquatic Science

Cite this