A candidate gene for Drosophila genome methylation

M. Narsa Reddy, Lin Ya Tang, Tai Lin Lee, C. K.James Shen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Like vertebrates, the genome of Drosophila melanogaster also contains methylated cytosines. However, the enzyme(s) responsible for this methylation has been elusive. By DNA transfection and sodium bisulfite sequencing, we show here that overexpression of dDnmt2, which is the only expressed and cloned Drosophila protein consisting of motifs conserved among the DNA cytosine methyltransferases, results in genomic DNA methylation of Drosophila S2 cells. The data provide the first evidence for dDnmt2 being one candidate gene encoding the Drosophila DNA methyltransferase(s).

Original languageEnglish
Pages (from-to)6301-6303
Number of pages3
JournalOncogene
Volume22
Issue number40
DOIs
Publication statusPublished - Sep 18 2003
Externally publishedYes

Keywords

  • DNA methylation
  • Drosophila cells
  • Enzyme

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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