A better biomaterial for bone regeneration

Type II collagen

Li Hsuan Chiu, Wen Fu T Lai, Yu Hui Tsai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Type II collagen is crucial to the development of embryonic skeletal system. Its functional defect in transgenic animal model results in severe chondrodysplasia.12 The in vitro studies including ours showed that type II collagen promotes chondrogenesis of mesenchymal stem cells (MSCs). 3-6 Our recent studies have demonstrated that type II collagen also regulated MSC adipogenesis and the early stages of osteogenesis.7 Type II collagen-coated surface enhanced osteogenesis-induced MSC calcium deposition, and increased protein expression levels of RUNX2. This mineralization-enhancing effect of type II collagen was diminished by MEK inhibitors. On the other hand, integrin α2β1 presents as the predominant receptor that responses to type II collagen in the osteogenic-induced MSCs. While implanted in the fracture bone of rats, the type II collagen-HA/TCP (nano-particle) scaffold group showed higher SFI scoring of foot-stepping and better locomotion as comparing to that of control group or type I collagen-HA/TCP group did. Collectively, type II collagen serves as an important modulator during early osteogenic differentiation of BMSCs, and enhance bone defect repair through an endochondral ossification-like process. The information advances our understanding about the cartilaginous ECM-BMSC interaction and provides perspective strategies for faster bone regeneration.

Original languageEnglish
Title of host publicationTechnical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
Pages343-346
Number of pages4
Volume3
Publication statusPublished - 2013
EventNanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 - Washington, DC, United States
Duration: May 12 2013May 16 2013

Conference

ConferenceNanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
CountryUnited States
CityWashington, DC
Period5/12/135/16/13

Fingerprint

Bone Regeneration
Collagen Type II
Biocompatible Materials
Mesenchymal Stromal Cells
Osteogenesis
Chondrogenesis
Adipogenesis
Genetically Modified Animals
Bone Fractures
Mitogen-Activated Protein Kinase Kinases
Locomotion
Collagen Type I
Integrins
Embryonic Development
Foot
Animal Models
Calcium
Bone and Bones
Control Groups

ASJC Scopus subject areas

  • Biotechnology

Cite this

Chiu, L. H., Lai, W. F. T., & Tsai, Y. H. (2013). A better biomaterial for bone regeneration: Type II collagen. In Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 (Vol. 3, pp. 343-346)

A better biomaterial for bone regeneration : Type II collagen. / Chiu, Li Hsuan; Lai, Wen Fu T; Tsai, Yu Hui.

Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. Vol. 3 2013. p. 343-346.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chiu, LH, Lai, WFT & Tsai, YH 2013, A better biomaterial for bone regeneration: Type II collagen. in Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. vol. 3, pp. 343-346, Nanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013, Washington, DC, United States, 5/12/13.
Chiu LH, Lai WFT, Tsai YH. A better biomaterial for bone regeneration: Type II collagen. In Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. Vol. 3. 2013. p. 343-346
Chiu, Li Hsuan ; Lai, Wen Fu T ; Tsai, Yu Hui. / A better biomaterial for bone regeneration : Type II collagen. Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. Vol. 3 2013. pp. 343-346
@inproceedings{c10119214fe144238e343ba656aa3fa1,
title = "A better biomaterial for bone regeneration: Type II collagen",
abstract = "Type II collagen is crucial to the development of embryonic skeletal system. Its functional defect in transgenic animal model results in severe chondrodysplasia.12 The in vitro studies including ours showed that type II collagen promotes chondrogenesis of mesenchymal stem cells (MSCs). 3-6 Our recent studies have demonstrated that type II collagen also regulated MSC adipogenesis and the early stages of osteogenesis.7 Type II collagen-coated surface enhanced osteogenesis-induced MSC calcium deposition, and increased protein expression levels of RUNX2. This mineralization-enhancing effect of type II collagen was diminished by MEK inhibitors. On the other hand, integrin α2β1 presents as the predominant receptor that responses to type II collagen in the osteogenic-induced MSCs. While implanted in the fracture bone of rats, the type II collagen-HA/TCP (nano-particle) scaffold group showed higher SFI scoring of foot-stepping and better locomotion as comparing to that of control group or type I collagen-HA/TCP group did. Collectively, type II collagen serves as an important modulator during early osteogenic differentiation of BMSCs, and enhance bone defect repair through an endochondral ossification-like process. The information advances our understanding about the cartilaginous ECM-BMSC interaction and provides perspective strategies for faster bone regeneration.",
author = "Chiu, {Li Hsuan} and Lai, {Wen Fu T} and Tsai, {Yu Hui}",
year = "2013",
language = "English",
isbn = "9781482205862",
volume = "3",
pages = "343--346",
booktitle = "Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013",

}

TY - GEN

T1 - A better biomaterial for bone regeneration

T2 - Type II collagen

AU - Chiu, Li Hsuan

AU - Lai, Wen Fu T

AU - Tsai, Yu Hui

PY - 2013

Y1 - 2013

N2 - Type II collagen is crucial to the development of embryonic skeletal system. Its functional defect in transgenic animal model results in severe chondrodysplasia.12 The in vitro studies including ours showed that type II collagen promotes chondrogenesis of mesenchymal stem cells (MSCs). 3-6 Our recent studies have demonstrated that type II collagen also regulated MSC adipogenesis and the early stages of osteogenesis.7 Type II collagen-coated surface enhanced osteogenesis-induced MSC calcium deposition, and increased protein expression levels of RUNX2. This mineralization-enhancing effect of type II collagen was diminished by MEK inhibitors. On the other hand, integrin α2β1 presents as the predominant receptor that responses to type II collagen in the osteogenic-induced MSCs. While implanted in the fracture bone of rats, the type II collagen-HA/TCP (nano-particle) scaffold group showed higher SFI scoring of foot-stepping and better locomotion as comparing to that of control group or type I collagen-HA/TCP group did. Collectively, type II collagen serves as an important modulator during early osteogenic differentiation of BMSCs, and enhance bone defect repair through an endochondral ossification-like process. The information advances our understanding about the cartilaginous ECM-BMSC interaction and provides perspective strategies for faster bone regeneration.

AB - Type II collagen is crucial to the development of embryonic skeletal system. Its functional defect in transgenic animal model results in severe chondrodysplasia.12 The in vitro studies including ours showed that type II collagen promotes chondrogenesis of mesenchymal stem cells (MSCs). 3-6 Our recent studies have demonstrated that type II collagen also regulated MSC adipogenesis and the early stages of osteogenesis.7 Type II collagen-coated surface enhanced osteogenesis-induced MSC calcium deposition, and increased protein expression levels of RUNX2. This mineralization-enhancing effect of type II collagen was diminished by MEK inhibitors. On the other hand, integrin α2β1 presents as the predominant receptor that responses to type II collagen in the osteogenic-induced MSCs. While implanted in the fracture bone of rats, the type II collagen-HA/TCP (nano-particle) scaffold group showed higher SFI scoring of foot-stepping and better locomotion as comparing to that of control group or type I collagen-HA/TCP group did. Collectively, type II collagen serves as an important modulator during early osteogenic differentiation of BMSCs, and enhance bone defect repair through an endochondral ossification-like process. The information advances our understanding about the cartilaginous ECM-BMSC interaction and provides perspective strategies for faster bone regeneration.

UR - http://www.scopus.com/inward/record.url?scp=84881102199&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881102199&partnerID=8YFLogxK

M3 - Conference contribution

SN - 9781482205862

VL - 3

SP - 343

EP - 346

BT - Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013

ER -