10 Citations (Scopus)

Abstract

We previously reported that 9-cis retinoic acid (RA) treatment induced an increase in mitochondrial (mt)DNA transcription. In order to extend these results, we tested various concentrations of 9-cis RA were used to treat 143B cells. Cells with low membrane potential treated with 9-cis RA showed significantly lower amounts of RXRα in mitochondria. We also found lower RXRα levels in mtDNA-depleted cells. Treating cells with 9-cis RA significantly increased expression of ND1, ND6, and COX I RNA. However, 9-cis RA-treatment did not appear to induce any significant changes in mtDNA copy number or mitochondrial mass. This study represents that 9-cis RA increases mtDNA transcription but not mtDNA replication, and it suggests that the effects of 9-cis RA on mitochondria are mediated by RXR localization to mitochondria. In addition, this is the first report that 9-cis RA regulation of RXR mitochondrial translocation depends on mitochondrial membrane potential and ATP.

Original languageEnglish
Pages (from-to)351-354
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume377
Issue number2
DOIs
Publication statusPublished - Dec 12 2008

Fingerprint

Retinoid X Receptors
Mitochondria
Transcription
Mitochondrial DNA
Membranes
alitretinoin
Mitochondrial Membrane Potential
Membrane Potentials
Adenosine Triphosphate

Keywords

  • 9-cis retinoic acid
  • Mitochondrial biogenesis
  • Retinoid X receptor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

@article{a2e17877b9e9406097f2a65bc730bca8,
title = "9-cis retinoic acid induces retinoid X receptor localized to the mitochondria for mediation of mitochondrial transcription",
abstract = "We previously reported that 9-cis retinoic acid (RA) treatment induced an increase in mitochondrial (mt)DNA transcription. In order to extend these results, we tested various concentrations of 9-cis RA were used to treat 143B cells. Cells with low membrane potential treated with 9-cis RA showed significantly lower amounts of RXRα in mitochondria. We also found lower RXRα levels in mtDNA-depleted cells. Treating cells with 9-cis RA significantly increased expression of ND1, ND6, and COX I RNA. However, 9-cis RA-treatment did not appear to induce any significant changes in mtDNA copy number or mitochondrial mass. This study represents that 9-cis RA increases mtDNA transcription but not mtDNA replication, and it suggests that the effects of 9-cis RA on mitochondria are mediated by RXR localization to mitochondria. In addition, this is the first report that 9-cis RA regulation of RXR mitochondrial translocation depends on mitochondrial membrane potential and ATP.",
keywords = "9-cis retinoic acid, Mitochondrial biogenesis, Retinoid X receptor",
author = "Lin, {Yung Wei} and Lien, {Li Ming} and Yeh, {Tien Shun} and Wu, {Hsiao Mei} and Liu, {Yi Li} and Hsieh, {Rong Hong}",
year = "2008",
month = "12",
day = "12",
doi = "10.1016/j.bbrc.2008.09.122",
language = "English",
volume = "377",
pages = "351--354",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier B.V.",
number = "2",

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TY - JOUR

T1 - 9-cis retinoic acid induces retinoid X receptor localized to the mitochondria for mediation of mitochondrial transcription

AU - Lin, Yung Wei

AU - Lien, Li Ming

AU - Yeh, Tien Shun

AU - Wu, Hsiao Mei

AU - Liu, Yi Li

AU - Hsieh, Rong Hong

PY - 2008/12/12

Y1 - 2008/12/12

N2 - We previously reported that 9-cis retinoic acid (RA) treatment induced an increase in mitochondrial (mt)DNA transcription. In order to extend these results, we tested various concentrations of 9-cis RA were used to treat 143B cells. Cells with low membrane potential treated with 9-cis RA showed significantly lower amounts of RXRα in mitochondria. We also found lower RXRα levels in mtDNA-depleted cells. Treating cells with 9-cis RA significantly increased expression of ND1, ND6, and COX I RNA. However, 9-cis RA-treatment did not appear to induce any significant changes in mtDNA copy number or mitochondrial mass. This study represents that 9-cis RA increases mtDNA transcription but not mtDNA replication, and it suggests that the effects of 9-cis RA on mitochondria are mediated by RXR localization to mitochondria. In addition, this is the first report that 9-cis RA regulation of RXR mitochondrial translocation depends on mitochondrial membrane potential and ATP.

AB - We previously reported that 9-cis retinoic acid (RA) treatment induced an increase in mitochondrial (mt)DNA transcription. In order to extend these results, we tested various concentrations of 9-cis RA were used to treat 143B cells. Cells with low membrane potential treated with 9-cis RA showed significantly lower amounts of RXRα in mitochondria. We also found lower RXRα levels in mtDNA-depleted cells. Treating cells with 9-cis RA significantly increased expression of ND1, ND6, and COX I RNA. However, 9-cis RA-treatment did not appear to induce any significant changes in mtDNA copy number or mitochondrial mass. This study represents that 9-cis RA increases mtDNA transcription but not mtDNA replication, and it suggests that the effects of 9-cis RA on mitochondria are mediated by RXR localization to mitochondria. In addition, this is the first report that 9-cis RA regulation of RXR mitochondrial translocation depends on mitochondrial membrane potential and ATP.

KW - 9-cis retinoic acid

KW - Mitochondrial biogenesis

KW - Retinoid X receptor

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U2 - 10.1016/j.bbrc.2008.09.122

DO - 10.1016/j.bbrc.2008.09.122

M3 - Article

VL - 377

SP - 351

EP - 354

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -