[5,10,15,20-Tetra-(N-methyl-3-pyridyl)porphyrin destabilizes the anti-parallel telomeric quadruplex d(TTAGGG)4].

I. V. Dutikova, O. F. Borisova, A. K. Shchelkina, J. Lin, S. Huang, A. A. Shtil', D. N. Kaliuzhnyǐ

Research output: Contribution to journalArticle

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Abstract

We studied the parameters of binding of 5,10,15,20-tetra-(N-methyl-3-pyridyl)porphyrin (TMPyP3) to the anti-parallel human telomeric G-quadruplex d(TTAGGG)4, the oligonucleotide dTTAGGGTTAGAG(TTAGGG)2 that does not form a quadruplex structure, as well as to the double stranded d(AC)8 x d(GT) and single stranded d(AC)8 and d(GT)8 DNAs. The analysis of absorption revealed that the binding constants and the number of DNA binding sites for TMPyP3 were d(AC)8 <d(GT)8 <d(AC)8 x d(GT)8 = d(TTAGGG)4 <dTTAGGGTTAGAG(TTAGGG)2. We demonstrated for the first time that the binding constant of TMPyP3 with the non-quadruplex chain dTTAGGGTTAGAG(TTAGGG)2 (1.3 x 10(7) M(-1)) is approximately 3 times bigger than the binding constant with the quadruplex d(TTAGGG)4 (4.6 x 10(6) M(-1)). Binding of two TMPyP3 molecules to d(TTAGGG)4 led to a decrease of thermostability of the G-quadruplex (deltaT(m) = -8 degrees C). Circular dichroism spectra of TMPyP3:d(TTAGGG)4 complexes revealed a shift of DNA structure from the G-quadruplex to an irregular chain. We hypothesize that partial destabilization of the telomeric G-quadruplex by TMPyP3 might be a reason for relatively low potency of this ligand as a telomerase inhibitor, as well as its marginal cytotoxicity for cultured tumor cells.

Original languageEnglish
Pages (from-to)929-937
Number of pages9
JournalMolekuliarnaia biologiia
Volume44
Issue number5
Publication statusPublished - Sep 2010
Externally publishedYes

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Porphyrins
G-Quadruplexes
Cultured Tumor Cells
Telomerase
DNA
Circular Dichroism
Oligonucleotides
Binding Sites
d(TTAGGG)4
Ligands

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Dutikova, I. V., Borisova, O. F., Shchelkina, A. K., Lin, J., Huang, S., Shtil', A. A., & Kaliuzhnyǐ, D. N. (2010). [5,10,15,20-Tetra-(N-methyl-3-pyridyl)porphyrin destabilizes the anti-parallel telomeric quadruplex d(TTAGGG)4]. Molekuliarnaia biologiia, 44(5), 929-937.

[5,10,15,20-Tetra-(N-methyl-3-pyridyl)porphyrin destabilizes the anti-parallel telomeric quadruplex d(TTAGGG)4]. / Dutikova, I. V.; Borisova, O. F.; Shchelkina, A. K.; Lin, J.; Huang, S.; Shtil', A. A.; Kaliuzhnyǐ, D. N.

In: Molekuliarnaia biologiia, Vol. 44, No. 5, 09.2010, p. 929-937.

Research output: Contribution to journalArticle

Dutikova, IV, Borisova, OF, Shchelkina, AK, Lin, J, Huang, S, Shtil', AA & Kaliuzhnyǐ, DN 2010, '[5,10,15,20-Tetra-(N-methyl-3-pyridyl)porphyrin destabilizes the anti-parallel telomeric quadruplex d(TTAGGG)4].', Molekuliarnaia biologiia, vol. 44, no. 5, pp. 929-937.
Dutikova, I. V. ; Borisova, O. F. ; Shchelkina, A. K. ; Lin, J. ; Huang, S. ; Shtil', A. A. ; Kaliuzhnyǐ, D. N. / [5,10,15,20-Tetra-(N-methyl-3-pyridyl)porphyrin destabilizes the anti-parallel telomeric quadruplex d(TTAGGG)4]. In: Molekuliarnaia biologiia. 2010 ; Vol. 44, No. 5. pp. 929-937.
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