5-HT modulates multiple conductances in immature rat rostral ventrolateral medulla neurones in vitro

L. L. Hwang, N. J. Dun

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

1. Whole-cell patch-clamp recordings were made from rostral ventrolateral medulla (RVLM) neurones of brainstem slices from 8- to 12-day-old rats. In the presence of tetrodotoxin (0.5 μM), 5-HT (50 μM) elicited an outward current (I(5-HT,outward)) (10/44 neurones) associated with an increase in membrane conductance, and an inward current (I(5-HT,inward)) (29/44 neurones) accompanied by a decrease or no significant change in membrane conductance. 2. The steady-state I-V relationship of I(5-HT,outward) showed an inward rectification; the 5-HT-induced current, which reversed at -87.9 ± 3.0 mV, was suppressed by 0.1 mM Ba2+. 3. Two types of steady-state I-V relationship for I(5-HT,inward) were noted: type I I(5-HT,inward) was characterized by a significant decrease in membrane conductance and reversed at a potential close to or negative to the theoretical K+ equilibrium potential (E(K)), -94 mV, in 8/17 neurones; type II I(5-HT,inward) was not associated with a significant change in membrane conductance and was relatively independent of membrane potential. 4. Both type I and type II I(5-HT,inward) were significantly reduced in a low [Na+](o) solution. In this solution, I(5-HT,inward) decreased with hyperpolarization and had a linear steady-state I-V relationship with a reversal potential of approximately -110 mV. The reversal potential of type I I(5-HT,inward) shifted to about -80 mV as the [K+](o) was increased from 3.1 to 7.0 mM in low [Na+](o) solution. The type II I(5-HT,inward) did not reverse at the estimated E(K) in the same solution. 5. While not affected by externally applied Cs+ (1 mM), I(5-HT,inward) was significantly smaller in RVLM neurones patched with Cs+-containing electrodes; the current reversed at -11.9 ± 6.4 mV in 8/15 responsive neurones. 6. It may be concluded that in rat RVLM neurones 5-HT increases an inwardly rectifying K+ conductance which may underlie the I(5-HT,outward) and that a combination of varying degrees of K+ conductance decrease and a Cs+-insensitive, non-selective cation conductance increase may account for the two types of conductance change associated with I(5-HT,inward).

Original languageEnglish
Pages (from-to)217-228
Number of pages12
JournalJournal of Physiology
Volume517
Issue number1
DOIs
Publication statusPublished - May 15 1999
Externally publishedYes

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Serotonin
Neurons
Membranes
In Vitro Techniques
Tetrodotoxin
Membrane Potentials
Brain Stem
Cations
Electrodes

ASJC Scopus subject areas

  • Physiology

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5-HT modulates multiple conductances in immature rat rostral ventrolateral medulla neurones in vitro. / Hwang, L. L.; Dun, N. J.

In: Journal of Physiology, Vol. 517, No. 1, 15.05.1999, p. 217-228.

Research output: Contribution to journalArticle

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N2 - 1. Whole-cell patch-clamp recordings were made from rostral ventrolateral medulla (RVLM) neurones of brainstem slices from 8- to 12-day-old rats. In the presence of tetrodotoxin (0.5 μM), 5-HT (50 μM) elicited an outward current (I(5-HT,outward)) (10/44 neurones) associated with an increase in membrane conductance, and an inward current (I(5-HT,inward)) (29/44 neurones) accompanied by a decrease or no significant change in membrane conductance. 2. The steady-state I-V relationship of I(5-HT,outward) showed an inward rectification; the 5-HT-induced current, which reversed at -87.9 ± 3.0 mV, was suppressed by 0.1 mM Ba2+. 3. Two types of steady-state I-V relationship for I(5-HT,inward) were noted: type I I(5-HT,inward) was characterized by a significant decrease in membrane conductance and reversed at a potential close to or negative to the theoretical K+ equilibrium potential (E(K)), -94 mV, in 8/17 neurones; type II I(5-HT,inward) was not associated with a significant change in membrane conductance and was relatively independent of membrane potential. 4. Both type I and type II I(5-HT,inward) were significantly reduced in a low [Na+](o) solution. In this solution, I(5-HT,inward) decreased with hyperpolarization and had a linear steady-state I-V relationship with a reversal potential of approximately -110 mV. The reversal potential of type I I(5-HT,inward) shifted to about -80 mV as the [K+](o) was increased from 3.1 to 7.0 mM in low [Na+](o) solution. The type II I(5-HT,inward) did not reverse at the estimated E(K) in the same solution. 5. While not affected by externally applied Cs+ (1 mM), I(5-HT,inward) was significantly smaller in RVLM neurones patched with Cs+-containing electrodes; the current reversed at -11.9 ± 6.4 mV in 8/15 responsive neurones. 6. It may be concluded that in rat RVLM neurones 5-HT increases an inwardly rectifying K+ conductance which may underlie the I(5-HT,outward) and that a combination of varying degrees of K+ conductance decrease and a Cs+-insensitive, non-selective cation conductance increase may account for the two types of conductance change associated with I(5-HT,inward).

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