3D co-cultured multicellular spheroids on microfluidic chip for studying ecm-mediated drug resistance

Venkanagouda S. Goudar, Long Sheng Lu, Manohar Prasad Koduri, Fan Gang Tseng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Among various ECM proteins, collagen type-I is a widely distributed extracellular matrix (ECM) protein expressed in different types of cancers and induces tumor growth and drug resistance. To prove this, in the present work we co-cultured colon cancer cells (HCT-8) and fibroblast cells (NIH3T3) in a PDMS based open welled micro 3D (μ-3D) cell culture chip. This co-culture model was employed to study the interaction of cancer cells and stromal cells and revealed that collagen treatment before chemotherapy can be a game-changer in a colon cancer treatment.

Original languageEnglish
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages853-854
Number of pages2
ISBN (Electronic)9781733419017
Publication statusPublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: Oct 4 2020Oct 9 2020

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period10/4/2010/9/20

Keywords

  • 3D spheroids
  • Collagen type-I
  • Colon cancer
  • ECM
  • μ-3D culture chip

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering
  • Chemistry(all)
  • Control and Systems Engineering

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