Abstract

Aim: To evaluate the effect of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) on cell proliferation and examine the mechanisms of THSG-enhanced proliferative potential in human dental pulp stem cells (hDPSC). Methodology: After treatment with THSG, hDPSC were collected. Cell viability was determined by MTS assay, while messenger RNA (mRNA) expressions of proliferation and stem cell markers were analyzed using real-time PCR. Flow cytometry was also conducted to analysis protein expression of stem cell markers. A colony-forming unit assay of hDPSC was carried out. Cellular telomerase activity was also identified using real-time PCR. In addition, proliferation-related proteins involved in the effects of THSG on hDPSC were analyzed by Western blotting. Data were analyzed using one-way analysis of variance and two-tailed Student's t-test. Results: Cell viability, colony-forming rates and telomerase activities of hDPSCs were enhanced after THSG treatment. mRNA expressions of proliferation markers (including expressions of NAD+-dependent histone deacetylase sirtuin 1 (SIRT1), proliferating cell nuclear antigen (PCNA), cyclin D1 and ribonucleotide reductase subunit M2 (RRM2)) increased significantly after THSG treatment (P < 0.05). Treatment with THSG for 3 h significantly augmented SIRT1 protein expression (P < 0.05). Furthermore, activities of proliferation-related proteins (including AMP-activated protein kinase (AMPK) and extracellular signal-regulated kinase (ERK) had also significantly increased at 3 h (P < 0.05). After THSG treatment, increased gene and protein expressions of pluripotent-like stem cell markers (including NANOG, OCT4, and SOX2) were observed. Conclusions: 2,3,5,4′-Tetrahydroxystilbene-2-O-β-glucoside treatment enhanced the renewal ability and proliferative potential of hDPSCs via the AMPK/ERK/SIRT1 axis, which may provide a novel autogenic cell-based therapeutic strategy in regenerative dentistry.

Original languageEnglish
Pages (from-to)1159-1170
Number of pages12
JournalInternational Endodontic Journal
Volume51
Issue number10
DOIs
Publication statusPublished - Oct 1 2018

Fingerprint

Sirtuin 1
Dental Pulp
AMP-Activated Protein Kinases
Mitogen-Activated Protein Kinase 3
Stem Cells
Telomerase
Proteins
Therapeutics
Real-Time Polymerase Chain Reaction
Cell Survival
Histone Deacetylase 1
Colony-Forming Units Assay
Pluripotent Stem Cells
Messenger RNA
Cyclin D1
Extracellular Signal-Regulated MAP Kinases
Proliferating Cell Nuclear Antigen
Dentistry
2,3,5,4'-tetrahydroxystilbene-2-O-glucoside
NAD

Keywords

  • 2,3,5,4′-tetrahydroxystilbene-2-O-β-glucoside
  • dental pulp stem cells
  • pluripotency
  • proliferation

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

2,3,5,4′-Tetrahydroxystilbene-2-O-β-glucoside potentiates self-renewal of human dental pulp stem cells via the AMPK/ERK/SIRT1 axis. / Lin, C. Y.; Chin, Y. T.; Kuo, P. J.; Lee, H. W.; Huang, H. M.; Lin, H. Y.; Weng, I. T.; Hsiung, C. N.; Chan, Y. H.; Lee, S. Y.

In: International Endodontic Journal, Vol. 51, No. 10, 01.10.2018, p. 1159-1170.

Research output: Contribution to journalArticle

@article{8de46caffd984f43afd122e9c45d8e0c,
title = "2,3,5,4′-Tetrahydroxystilbene-2-O-β-glucoside potentiates self-renewal of human dental pulp stem cells via the AMPK/ERK/SIRT1 axis",
abstract = "Aim: To evaluate the effect of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) on cell proliferation and examine the mechanisms of THSG-enhanced proliferative potential in human dental pulp stem cells (hDPSC). Methodology: After treatment with THSG, hDPSC were collected. Cell viability was determined by MTS assay, while messenger RNA (mRNA) expressions of proliferation and stem cell markers were analyzed using real-time PCR. Flow cytometry was also conducted to analysis protein expression of stem cell markers. A colony-forming unit assay of hDPSC was carried out. Cellular telomerase activity was also identified using real-time PCR. In addition, proliferation-related proteins involved in the effects of THSG on hDPSC were analyzed by Western blotting. Data were analyzed using one-way analysis of variance and two-tailed Student's t-test. Results: Cell viability, colony-forming rates and telomerase activities of hDPSCs were enhanced after THSG treatment. mRNA expressions of proliferation markers (including expressions of NAD+-dependent histone deacetylase sirtuin 1 (SIRT1), proliferating cell nuclear antigen (PCNA), cyclin D1 and ribonucleotide reductase subunit M2 (RRM2)) increased significantly after THSG treatment (P < 0.05). Treatment with THSG for 3 h significantly augmented SIRT1 protein expression (P < 0.05). Furthermore, activities of proliferation-related proteins (including AMP-activated protein kinase (AMPK) and extracellular signal-regulated kinase (ERK) had also significantly increased at 3 h (P < 0.05). After THSG treatment, increased gene and protein expressions of pluripotent-like stem cell markers (including NANOG, OCT4, and SOX2) were observed. Conclusions: 2,3,5,4′-Tetrahydroxystilbene-2-O-β-glucoside treatment enhanced the renewal ability and proliferative potential of hDPSCs via the AMPK/ERK/SIRT1 axis, which may provide a novel autogenic cell-based therapeutic strategy in regenerative dentistry.",
keywords = "2,3,5,4′-tetrahydroxystilbene-2-O-β-glucoside, dental pulp stem cells, pluripotency, proliferation",
author = "Lin, {C. Y.} and Chin, {Y. T.} and Kuo, {P. J.} and Lee, {H. W.} and Huang, {H. M.} and Lin, {H. Y.} and Weng, {I. T.} and Hsiung, {C. N.} and Chan, {Y. H.} and Lee, {S. Y.}",
year = "2018",
month = "10",
day = "1",
doi = "10.1111/iej.12935",
language = "English",
volume = "51",
pages = "1159--1170",
journal = "International Endodontic Journal",
issn = "0143-2885",
publisher = "Wiley-Blackwell",
number = "10",

}

TY - JOUR

T1 - 2,3,5,4′-Tetrahydroxystilbene-2-O-β-glucoside potentiates self-renewal of human dental pulp stem cells via the AMPK/ERK/SIRT1 axis

AU - Lin, C. Y.

AU - Chin, Y. T.

AU - Kuo, P. J.

AU - Lee, H. W.

AU - Huang, H. M.

AU - Lin, H. Y.

AU - Weng, I. T.

AU - Hsiung, C. N.

AU - Chan, Y. H.

AU - Lee, S. Y.

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Aim: To evaluate the effect of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) on cell proliferation and examine the mechanisms of THSG-enhanced proliferative potential in human dental pulp stem cells (hDPSC). Methodology: After treatment with THSG, hDPSC were collected. Cell viability was determined by MTS assay, while messenger RNA (mRNA) expressions of proliferation and stem cell markers were analyzed using real-time PCR. Flow cytometry was also conducted to analysis protein expression of stem cell markers. A colony-forming unit assay of hDPSC was carried out. Cellular telomerase activity was also identified using real-time PCR. In addition, proliferation-related proteins involved in the effects of THSG on hDPSC were analyzed by Western blotting. Data were analyzed using one-way analysis of variance and two-tailed Student's t-test. Results: Cell viability, colony-forming rates and telomerase activities of hDPSCs were enhanced after THSG treatment. mRNA expressions of proliferation markers (including expressions of NAD+-dependent histone deacetylase sirtuin 1 (SIRT1), proliferating cell nuclear antigen (PCNA), cyclin D1 and ribonucleotide reductase subunit M2 (RRM2)) increased significantly after THSG treatment (P < 0.05). Treatment with THSG for 3 h significantly augmented SIRT1 protein expression (P < 0.05). Furthermore, activities of proliferation-related proteins (including AMP-activated protein kinase (AMPK) and extracellular signal-regulated kinase (ERK) had also significantly increased at 3 h (P < 0.05). After THSG treatment, increased gene and protein expressions of pluripotent-like stem cell markers (including NANOG, OCT4, and SOX2) were observed. Conclusions: 2,3,5,4′-Tetrahydroxystilbene-2-O-β-glucoside treatment enhanced the renewal ability and proliferative potential of hDPSCs via the AMPK/ERK/SIRT1 axis, which may provide a novel autogenic cell-based therapeutic strategy in regenerative dentistry.

AB - Aim: To evaluate the effect of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) on cell proliferation and examine the mechanisms of THSG-enhanced proliferative potential in human dental pulp stem cells (hDPSC). Methodology: After treatment with THSG, hDPSC were collected. Cell viability was determined by MTS assay, while messenger RNA (mRNA) expressions of proliferation and stem cell markers were analyzed using real-time PCR. Flow cytometry was also conducted to analysis protein expression of stem cell markers. A colony-forming unit assay of hDPSC was carried out. Cellular telomerase activity was also identified using real-time PCR. In addition, proliferation-related proteins involved in the effects of THSG on hDPSC were analyzed by Western blotting. Data were analyzed using one-way analysis of variance and two-tailed Student's t-test. Results: Cell viability, colony-forming rates and telomerase activities of hDPSCs were enhanced after THSG treatment. mRNA expressions of proliferation markers (including expressions of NAD+-dependent histone deacetylase sirtuin 1 (SIRT1), proliferating cell nuclear antigen (PCNA), cyclin D1 and ribonucleotide reductase subunit M2 (RRM2)) increased significantly after THSG treatment (P < 0.05). Treatment with THSG for 3 h significantly augmented SIRT1 protein expression (P < 0.05). Furthermore, activities of proliferation-related proteins (including AMP-activated protein kinase (AMPK) and extracellular signal-regulated kinase (ERK) had also significantly increased at 3 h (P < 0.05). After THSG treatment, increased gene and protein expressions of pluripotent-like stem cell markers (including NANOG, OCT4, and SOX2) were observed. Conclusions: 2,3,5,4′-Tetrahydroxystilbene-2-O-β-glucoside treatment enhanced the renewal ability and proliferative potential of hDPSCs via the AMPK/ERK/SIRT1 axis, which may provide a novel autogenic cell-based therapeutic strategy in regenerative dentistry.

KW - 2,3,5,4′-tetrahydroxystilbene-2-O-β-glucoside

KW - dental pulp stem cells

KW - pluripotency

KW - proliferation

UR - http://www.scopus.com/inward/record.url?scp=85052949487&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85052949487&partnerID=8YFLogxK

U2 - 10.1111/iej.12935

DO - 10.1111/iej.12935

M3 - Article

AN - SCOPUS:85052949487

VL - 51

SP - 1159

EP - 1170

JO - International Endodontic Journal

JF - International Endodontic Journal

SN - 0143-2885

IS - 10

ER -