Abstract

Periodontitis, a chronic infection by periodontopathic bacteria, induces uncontrolled inflammation, which leads to periodontal tissue destruction. 2,3,5,4′-Tetrahydroxystilbene-2-O-beta-glucoside (THSG), a polyphenol extracted from Polygoni Multiflori, reportedly has anti-inflammatory properties. In this study, we investigated the mechanisms of THSG on the Porphyromonas gingivalis-induced inflammatory responses in human gingival fibroblasts and animal modeling of ligature-induced periodontitis. Human gingival fibroblast cells were treated with lipopolysaccharide (LPS) extracted from P. gingivalis in the presence of resveratrol or THSG to analyze the expression of TNF-α, IL-1β, and IL-6 genes. Increased AMP-activated protein kinase (AMPK) activation and SirT1 expression were induced by THSG. Treatment of THSG decreased the expression of LPS-induced inflammatory cytokines, enhanced AMPK activation, and increased the expression of SirT1. In addition, it suppressed the activation of NF-B when cells were stimulated with P. gingivalis LPS. The anti-inflammatory effect of THSG and P. Multiflori crude extracts was reproduced in ligature-induced periodontitis animal modeling. In conclusion, THSG inhibited the inflammatory responses of P. gingivalis-stimulated human gingival fibroblasts and ameliorated ligature-induced periodontitis in animal model.

Original languageEnglish
Article number6953459
JournalMediators of Inflammation
Volume2016
DOIs
Publication statusPublished - 2016

Fingerprint

Porphyromonas gingivalis
Periodontitis
Ligation
Lipopolysaccharides
AMP-Activated Protein Kinases
Fibroblasts
Anti-Inflammatory Agents
Chronic Periodontitis
Polyphenols
Complex Mixtures
Interleukin-1
Interleukin-6
Animal Models
Cytokines
Inflammation
Bacteria
2,3,5,4'-tetrahydroxystilbene-2-O-glucoside
Infection
Genes

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

2,3,5,4′-tetrahydroxystilbene-2-O- β -glucoside isolated from Polygoni Multiflori ameliorates the development of periodontitis. / Chin, Yu Tang; Hsieh, Meng Ti; Lin, Chi Yu; Kuo, Po Jan; Yang, Yu Chen S H; Shih, Ya Jung; Lai, Hsuan Yu; Cheng, Guei Yun; Tang, Heng Yuan; Lee, Chen Chen; Lee, Sheng Yang; Wang, Ching Chiung; Lin, Hung Yun; Fu, Earl; Whang-Peng, Jacqueline; Liu, Leroy F.

In: Mediators of Inflammation, Vol. 2016, 6953459, 2016.

Research output: Contribution to journalArticle

Chin, Yu Tang ; Hsieh, Meng Ti ; Lin, Chi Yu ; Kuo, Po Jan ; Yang, Yu Chen S H ; Shih, Ya Jung ; Lai, Hsuan Yu ; Cheng, Guei Yun ; Tang, Heng Yuan ; Lee, Chen Chen ; Lee, Sheng Yang ; Wang, Ching Chiung ; Lin, Hung Yun ; Fu, Earl ; Whang-Peng, Jacqueline ; Liu, Leroy F. / 2,3,5,4′-tetrahydroxystilbene-2-O- β -glucoside isolated from Polygoni Multiflori ameliorates the development of periodontitis. In: Mediators of Inflammation. 2016 ; Vol. 2016.
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abstract = "Periodontitis, a chronic infection by periodontopathic bacteria, induces uncontrolled inflammation, which leads to periodontal tissue destruction. 2,3,5,4′-Tetrahydroxystilbene-2-O-beta-glucoside (THSG), a polyphenol extracted from Polygoni Multiflori, reportedly has anti-inflammatory properties. In this study, we investigated the mechanisms of THSG on the Porphyromonas gingivalis-induced inflammatory responses in human gingival fibroblasts and animal modeling of ligature-induced periodontitis. Human gingival fibroblast cells were treated with lipopolysaccharide (LPS) extracted from P. gingivalis in the presence of resveratrol or THSG to analyze the expression of TNF-α, IL-1β, and IL-6 genes. Increased AMP-activated protein kinase (AMPK) activation and SirT1 expression were induced by THSG. Treatment of THSG decreased the expression of LPS-induced inflammatory cytokines, enhanced AMPK activation, and increased the expression of SirT1. In addition, it suppressed the activation of NF-B when cells were stimulated with P. gingivalis LPS. The anti-inflammatory effect of THSG and P. Multiflori crude extracts was reproduced in ligature-induced periodontitis animal modeling. In conclusion, THSG inhibited the inflammatory responses of P. gingivalis-stimulated human gingival fibroblasts and ameliorated ligature-induced periodontitis in animal model.",
author = "Chin, {Yu Tang} and Hsieh, {Meng Ti} and Lin, {Chi Yu} and Kuo, {Po Jan} and Yang, {Yu Chen S H} and Shih, {Ya Jung} and Lai, {Hsuan Yu} and Cheng, {Guei Yun} and Tang, {Heng Yuan} and Lee, {Chen Chen} and Lee, {Sheng Yang} and Wang, {Ching Chiung} and Lin, {Hung Yun} and Earl Fu and Jacqueline Whang-Peng and Liu, {Leroy F.}",
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AU - Chin, Yu Tang

AU - Hsieh, Meng Ti

AU - Lin, Chi Yu

AU - Kuo, Po Jan

AU - Yang, Yu Chen S H

AU - Shih, Ya Jung

AU - Lai, Hsuan Yu

AU - Cheng, Guei Yun

AU - Tang, Heng Yuan

AU - Lee, Chen Chen

AU - Lee, Sheng Yang

AU - Wang, Ching Chiung

AU - Lin, Hung Yun

AU - Fu, Earl

AU - Whang-Peng, Jacqueline

AU - Liu, Leroy F.

PY - 2016

Y1 - 2016

N2 - Periodontitis, a chronic infection by periodontopathic bacteria, induces uncontrolled inflammation, which leads to periodontal tissue destruction. 2,3,5,4′-Tetrahydroxystilbene-2-O-beta-glucoside (THSG), a polyphenol extracted from Polygoni Multiflori, reportedly has anti-inflammatory properties. In this study, we investigated the mechanisms of THSG on the Porphyromonas gingivalis-induced inflammatory responses in human gingival fibroblasts and animal modeling of ligature-induced periodontitis. Human gingival fibroblast cells were treated with lipopolysaccharide (LPS) extracted from P. gingivalis in the presence of resveratrol or THSG to analyze the expression of TNF-α, IL-1β, and IL-6 genes. Increased AMP-activated protein kinase (AMPK) activation and SirT1 expression were induced by THSG. Treatment of THSG decreased the expression of LPS-induced inflammatory cytokines, enhanced AMPK activation, and increased the expression of SirT1. In addition, it suppressed the activation of NF-B when cells were stimulated with P. gingivalis LPS. The anti-inflammatory effect of THSG and P. Multiflori crude extracts was reproduced in ligature-induced periodontitis animal modeling. In conclusion, THSG inhibited the inflammatory responses of P. gingivalis-stimulated human gingival fibroblasts and ameliorated ligature-induced periodontitis in animal model.

AB - Periodontitis, a chronic infection by periodontopathic bacteria, induces uncontrolled inflammation, which leads to periodontal tissue destruction. 2,3,5,4′-Tetrahydroxystilbene-2-O-beta-glucoside (THSG), a polyphenol extracted from Polygoni Multiflori, reportedly has anti-inflammatory properties. In this study, we investigated the mechanisms of THSG on the Porphyromonas gingivalis-induced inflammatory responses in human gingival fibroblasts and animal modeling of ligature-induced periodontitis. Human gingival fibroblast cells were treated with lipopolysaccharide (LPS) extracted from P. gingivalis in the presence of resveratrol or THSG to analyze the expression of TNF-α, IL-1β, and IL-6 genes. Increased AMP-activated protein kinase (AMPK) activation and SirT1 expression were induced by THSG. Treatment of THSG decreased the expression of LPS-induced inflammatory cytokines, enhanced AMPK activation, and increased the expression of SirT1. In addition, it suppressed the activation of NF-B when cells were stimulated with P. gingivalis LPS. The anti-inflammatory effect of THSG and P. Multiflori crude extracts was reproduced in ligature-induced periodontitis animal modeling. In conclusion, THSG inhibited the inflammatory responses of P. gingivalis-stimulated human gingival fibroblasts and ameliorated ligature-induced periodontitis in animal model.

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