2,2′-Diphenyl-1-picrylhydrazyl radical-scavenging active components from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) hulls

Ching Chuan Kuo, Wenchang Chiang, Guey Ping Liu, Ya Lin Chien, Jang Yang Chang, Ching Kuo Lee, Jir Mehng Lo, Shou Ling Huang, Ming Chih Shih, Yueh Hsiung Kuo

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69 Citations (Scopus)

Abstract

An activity-directed fractionation and purification process was used to identify the antioxidative components of adlay hulls. Hulls of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) were extracted with methanol and then separated into water, 1-butanol, ethyl acetate, and hexane fractions. The 1-butanol-soluble fraction exhibited greater capacity to scavenge 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radicals when compared with fractions soluble in water, ethyl acetate, and hexane phases. The 1-butanol fraction was then subjected to separation and purification using Diaion HP-20 chromatography, silica gel chromatography, and HPLC. Six compounds showing strong antioxidant activity were identified by spectroscopic methods (1H NMR, 13C NMR, IR, and MS) and by comparison with authentic samples to be coniferyl alcohol (1), syringic acid (2), ferulic acid (3), syringaresinol (4), 4-ketopinoresinol (5), and a new lignan, mayuenolide (6).

Original languageEnglish
Pages (from-to)5850-5855
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume50
Issue number21
DOIs
Publication statusPublished - Oct 9 2002
Externally publishedYes

Keywords

  • Active components
  • Adlay hulls
  • Antioxidant activity
  • Free radical

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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    Kuo, C. C., Chiang, W., Liu, G. P., Chien, Y. L., Chang, J. Y., Lee, C. K., Lo, J. M., Huang, S. L., Shih, M. C., & Kuo, Y. H. (2002). 2,2′-Diphenyl-1-picrylhydrazyl radical-scavenging active components from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) hulls. Journal of Agricultural and Food Chemistry, 50(21), 5850-5855. https://doi.org/10.1021/jf020391w