2,2′-Diphenyl-1-picrylhydrazyl radical-scavenging active components from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) hulls

Ching Chuan Kuo; Wenchang Chiang; Guey Ping Liu; Ya Lin Chien; Jang Yang Chang; Ching Kuo Lee; Jir Mehng Lo; Shou Ling Huang; Ming Chih Shih; Yueh Hsiung Kuo

doi:10.1021/jf020391w

2,2′-Diphenyl-1-picrylhydrazyl radical-scavenging active components from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) hulls

Ching Chuan Kuo, Wenchang Chiang, Guey Ping Liu, Ya Lin Chien, Jang Yang Chang, Ching Kuo Lee, Jir Mehng Lo, Shou Ling Huang, Ming Chih Shih, Yueh Hsiung Kuo

Research output: Contribution to journal › Article › peer-review

80 Citations (Scopus)

Abstract

An activity-directed fractionation and purification process was used to identify the antioxidative components of adlay hulls. Hulls of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) were extracted with methanol and then separated into water, 1-butanol, ethyl acetate, and hexane fractions. The 1-butanol-soluble fraction exhibited greater capacity to scavenge 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radicals when compared with fractions soluble in water, ethyl acetate, and hexane phases. The 1-butanol fraction was then subjected to separation and purification using Diaion HP-20 chromatography, silica gel chromatography, and HPLC. Six compounds showing strong antioxidant activity were identified by spectroscopic methods (¹H NMR, ¹³C NMR, IR, and MS) and by comparison with authentic samples to be coniferyl alcohol (1), syringic acid (2), ferulic acid (3), syringaresinol (4), 4-ketopinoresinol (5), and a new lignan, mayuenolide (6).

Original language	English
Pages (from-to)	5850-5855
Number of pages	6
Journal	Journal of Agricultural and Food Chemistry
Volume	50
Issue number	21
DOIs	https://doi.org/10.1021/jf020391w
Publication status	Published - Oct 9 2002
Externally published	Yes

Keywords

Active components
Adlay hulls
Antioxidant activity
Free radical

ASJC Scopus subject areas

Agricultural and Biological Sciences (miscellaneous)
Food Science
Chemistry (miscellaneous)

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10.1021/jf020391w

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title = "2,2′-Diphenyl-1-picrylhydrazyl radical-scavenging active components from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) hulls",

abstract = "An activity-directed fractionation and purification process was used to identify the antioxidative components of adlay hulls. Hulls of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) were extracted with methanol and then separated into water, 1-butanol, ethyl acetate, and hexane fractions. The 1-butanol-soluble fraction exhibited greater capacity to scavenge 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radicals when compared with fractions soluble in water, ethyl acetate, and hexane phases. The 1-butanol fraction was then subjected to separation and purification using Diaion HP-20 chromatography, silica gel chromatography, and HPLC. Six compounds showing strong antioxidant activity were identified by spectroscopic methods (1H NMR, 13C NMR, IR, and MS) and by comparison with authentic samples to be coniferyl alcohol (1), syringic acid (2), ferulic acid (3), syringaresinol (4), 4-ketopinoresinol (5), and a new lignan, mayuenolide (6).",

keywords = "Active components, Adlay hulls, Antioxidant activity, Free radical",

author = "Kuo, {Ching Chuan} and Wenchang Chiang and Liu, {Guey Ping} and Chien, {Ya Lin} and Chang, {Jang Yang} and Lee, {Ching Kuo} and Lo, {Jir Mehng} and Huang, {Shou Ling} and Shih, {Ming Chih} and Kuo, {Yueh Hsiung}",

year = "2002",

month = oct,

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doi = "10.1021/jf020391w",

language = "English",

volume = "50",

pages = "5850--5855",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

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T1 - 2,2′-Diphenyl-1-picrylhydrazyl radical-scavenging active components from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) hulls

AU - Kuo, Ching Chuan

AU - Chiang, Wenchang

AU - Liu, Guey Ping

AU - Chien, Ya Lin

AU - Chang, Jang Yang

AU - Lee, Ching Kuo

AU - Lo, Jir Mehng

AU - Huang, Shou Ling

AU - Shih, Ming Chih

AU - Kuo, Yueh Hsiung

PY - 2002/10/9

Y1 - 2002/10/9

N2 - An activity-directed fractionation and purification process was used to identify the antioxidative components of adlay hulls. Hulls of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) were extracted with methanol and then separated into water, 1-butanol, ethyl acetate, and hexane fractions. The 1-butanol-soluble fraction exhibited greater capacity to scavenge 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radicals when compared with fractions soluble in water, ethyl acetate, and hexane phases. The 1-butanol fraction was then subjected to separation and purification using Diaion HP-20 chromatography, silica gel chromatography, and HPLC. Six compounds showing strong antioxidant activity were identified by spectroscopic methods (1H NMR, 13C NMR, IR, and MS) and by comparison with authentic samples to be coniferyl alcohol (1), syringic acid (2), ferulic acid (3), syringaresinol (4), 4-ketopinoresinol (5), and a new lignan, mayuenolide (6).

AB - An activity-directed fractionation and purification process was used to identify the antioxidative components of adlay hulls. Hulls of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) were extracted with methanol and then separated into water, 1-butanol, ethyl acetate, and hexane fractions. The 1-butanol-soluble fraction exhibited greater capacity to scavenge 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radicals when compared with fractions soluble in water, ethyl acetate, and hexane phases. The 1-butanol fraction was then subjected to separation and purification using Diaion HP-20 chromatography, silica gel chromatography, and HPLC. Six compounds showing strong antioxidant activity were identified by spectroscopic methods (1H NMR, 13C NMR, IR, and MS) and by comparison with authentic samples to be coniferyl alcohol (1), syringic acid (2), ferulic acid (3), syringaresinol (4), 4-ketopinoresinol (5), and a new lignan, mayuenolide (6).

KW - Active components

KW - Adlay hulls

KW - Antioxidant activity

KW - Free radical

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2,2′-Diphenyl-1-picrylhydrazyl radical-scavenging active components from adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) hulls

Abstract

Keywords

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