@article{c75bb1dca95f4d749a3e9af47ade2879,
title = "10′(Z),13′(E)-Heptadecadienylhydroquinone Inhibits Swarming and Virulence Factors and Increases Polymyxin B Susceptibility in Proteus mirabilis",
abstract = "In this study, we demonstrated that 10′(Z), 13′(E)-heptadecadienylhydroquinone (HQ17-2), isolated from the lacquer tree, could decrease swarming motility and hemolysin activity but increase polymyxin B (PB) susceptibilityof Proteus mirabilis which is intrinsically highly-resistant to PB. The increased PB susceptibility induced by HQ17-2 was also observed in clinical isolates and biofilm-grown cells. HQ17-2 could inhibit swarming in the wild-type and rppA mutant but not in the rcsB mutant, indicating that HQ17-2 inhibits swarming through the RcsB-dependent pathway, a two-component signaling pathway negatively regulating swarming and virulence factor expression. The inhibition of hemolysin activity by HQ17-2 is also mediated through the RcsB-dependent pathway, because HQ17-2 could not inhibit hemolysin activity in the rcsB mutant. Moreover, the finding that HQ17-2 inhibits the expression of flhDC gene in the wild-type and rcsB-complemented strain but not in the rcsB mutant supports the notion. By contrast, HQ17-2 could increase PB susceptibility in the wild-type and rcsB mutant but not in the rppA mutant, indicating that HQ17-2 increases PB susceptibility through the RppA-dependent pathway, a signaling pathway positively regulating PB resistance. In addition, HQ17-2 could inhibit the promoter activities of rppA and pmrI, a gene positively regulated by RppA and involved in PB resistance, in the wild-type but not in the rppA mutant. The inhibition of rppA and pmrI expression caused lipopolysaccharide purified from HQ17-2-treated cells to have higher affinity for PB. Altogether, this study uncovers new biological effects of HQ17-2 and provides evidence for the potential of HQ17-2 in clinical applications. {\textcopyright} 2012 Liu et al.",
keywords = "10',13' heptadecadienylhydroquinone, ampicillin, bacterial protein, ciprofloxacin, gentamicin, hemolysin, hydroquinone derivative, kanamycin, lipopolysaccharide, polymyxin B, protein rcsB, protein rppA, streptomycin, tetracycline, unclassified drug, virulence factor, 10'(Z),13'(E) heptadecadienylhydroquinone, 10'(Z),13'(E)-heptadecadienylhydroquinone, acyltransferase, antiinfective agent, chalcone synthase, RcsB protein, Bacteria, antibiotic resistance, antibiotic sensitivity, article, bacterial cell, bacterial gene, bacterial phenomena and functions, bacterial strain, bacterium isolate, bacterium mutant, binding affinity, biofilm, controlled study, flhDC gene, gene expression regulation, minimum inhibitory concentration, nonhuman, pmrl gene, promoter region, protein expression, Proteus mirabilis, rppA gene, signal transduction, swarming motility, wild type, drug effect, drug potentiation, genetics, metabolism, microbial sensitivity test, mutation, Acyltransferases, Anti-Bacterial Agents, Bacterial Proteins, Drug Synergism, Gene Expression Regulation, Bacterial, Hemolysin Proteins, Hydroquinones, Microbial Sensitivity Tests, Mutation, Polymyxin B, Promoter Regions, Genetic, Signal Transduction, Virulence Factors",
author = "Ming-Che Liu and Shwu-Bin Lin and Hsiung-Fei Chien and Won-Bo Wang and Yu-Han Yuan and Po-Ren Hsueh and Shwu-Jen Liaw",
note = "被引用次數：3 Export Date: 16 March 2016 通訊地址: Liaw, S.-J.; Department and Graduate Institute of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University, Taipei, Taiwan; 電子郵件： sjliaw@ntu.edu.tw 化學物質/CAS: ampicillin, 69-52-3, 69-53-4, 7177-48-2, 74083-13-9, 94586-58-0; ciprofloxacin, 85721-33-1; gentamicin, 1392-48-9, 1403-66-3, 1405-41-0; kanamycin, 11025-66-4, 61230-38-4, 8063-07-8; polymyxin B, 1404-26-8, 1405-20-5; streptomycin, 57-92-1; tetracycline, 23843-90-5, 60-54-8, 64-75-5, 8021-86-1; acyltransferase, 9012-30-0, 9054-54-0; chalcone synthase, 56803-04-4; 10'(Z),13'(E)-heptadecadienylhydroquinone; Acyltransferases, 2.3.-; Anti-Bacterial Agents; Bacterial Proteins; Hemolysin Proteins; Hydroquinones; Polymyxin B, 1404-26-8; RcsB protein, Bacteria, 127737-30-8; Virulence Factors; flavanone synthetase, 2.3.1.74 製造商： Fluka, United States 參考文獻: Jacobsen, S.M., Stickler, D.J., Mobley, H.L., Shirtliff, M.E., Complicated catheter-associated urinary tract infections due to Escherichia coli and Proteus mirabilis (2008) Clin Microbiol Rev, 21, pp. 26-59; 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year = "2012",
doi = "10.1371/journal.pone.0045563",
language = "English",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",
}