1-Benzyl-2-Phenylbenzimidazole (BPB), a Benzimidazole derivative, induces cell apoptosis in human chondrosarcoma through intrinsic and extrinsic pathways

Ju Fang Liu, Yuan Li Huang, Wei Hung Yang, Chih Shiang Chang, Chih Hsin Tang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In this study, we investigated the anticancer effects of a new benzimidazole derivative, 1-benzyl-2-phenyl -benzimidazole (BPB), in human chondrosarcoma cells. BPB-mediated apoptosis was assessed by the MTT assay and flow cytometry analysis. The in vivo efficacy was examined in a JJ012 xenograft model. Here we found that BPB induced apoptosis in human chondrosarcoma cell lines (JJ012 and SW1353) but not in primary chondrocytes. BPB induced upregulation of Bax, Bad and Bak, downregulation of Bcl-2, Bid and Bcl-XL and dysfunction of mitochondria in chondrosarcoma. In addition, BPB also promoted cytosolic releases AIF and Endo G. Furthermore, it triggered extrinsic death receptor-dependent pathway, which was characterized by activating Fas, FADD and caspase-8. Most importantly, animal studies revealed a dramatic 40% reduction in tumor volume after 21 days of treatment. Thus, BPB may be a novel anticancer agent for the treatment of chondrosarcoma.

Original languageEnglish
Pages (from-to)16472-16488
Number of pages17
JournalInternational Journal of Molecular Sciences
Volume13
Issue number12
DOIs
Publication statusPublished - Dec 1 2012

Keywords

  • Benzimidazole
  • Chinese herb
  • Chondrosarcoma
  • Extrinsic pathway
  • Intrinsic pathway

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Fingerprint Dive into the research topics of '1-Benzyl-2-Phenylbenzimidazole (BPB), a Benzimidazole derivative, induces cell apoptosis in human chondrosarcoma through intrinsic and extrinsic pathways'. Together they form a unique fingerprint.

  • Cite this