同類脫鈣冷凍乾燥骨併用組織導引再生法的齒槽骨再生促進作用

Several kinds of treatment methods, including bone grafting and guided tissue regeneration (GTR) technique, have been used in the treatment of oral bony defects. For the time being, one of the bone grafting materials, demineralized freeze-dried bone allograft (DFDBA), has been applied in conjunction with GTR technique in order to achieve further new bone formation. However, it is still enigmatic to realize the tissue response to DFDBA in bony defects when it is co-treated with GTR technique. After protein analysis of Dembone, one commercial product of human DFDBA, and scanning electron microscopic examination of Gore- Tex membrane, one telflon membrane used in GTR procedure. Six volunteer patients with a deep bony defect around their bicuspids, which evaluated hopeless in periodontal treatment, are selected. After the patients accepted inflammatory control of periodontal disease, a surgical flap with be raised around the selected tooth and with Gore-Tex membrane been hermetically suspended on the thoroughly treated root surface. As mixed with normal saline, Dembone is carried to the bony defect underneath the membrane and filled to the level of bone margin. The surgical wound is primarily closed with sutures. The new tissues growing in the periodonta1 defect were harvested three weeks after the surgery is performed and processed for the observation under light microscopic, histochemical and electron microscopic examination. In light microscopic and histochemica1 examinations, many capillaries, fiberous tissues, inflamation cells, and a few multinuclear cells were found surrounding Dembone particles. No newbone formation was found surrounding Dembone particles during whole experimental periods. The osteogenetic effects of DFDBA n human alveolar bony defects may not be osteoindutive as those in bone morphogenetic protein.