κ-Carrageenan Gel Modified Mesoporous Gold Chronocoulometric Sensor for Ultrasensitive Detection of MicroRNA

Bidita Salahuddin, Mostafa Kamal Masud, Shazed Aziz, Chia Hung Liu, Nasim Amiralian, Aditya Ashok, S. M.Azad Hossain, Hyeongyu Park, Md Abdul Wahab, Mohammed A. Amin, M. Adharvana Chari, Alan E. Rowan, Yusuke Yamauchi, Md Shahriar A. Hossain, Yusuf Valentino Kaneti

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Hydrogel-functionalized surface-based transducers demonstrate fluid-like kinetics, non-fouling properties, and superior biocompatibility. The integration of such properties of threedimensional (3D) micro- or macrostructure of hydrogels into a mesoporous platform provides a favorable moiety for incorporating biomolecule for adsorption or hybridization with a capture agent. Herein, we report a novel κ-carrageenan hydrogelcoated mesoporous gold (Au) electrode (abbreviated as MPGE/ gel) for chronocoulometric (CC) detection of microRNA (miRNA). The κ-carrageenan gel provides a 3D porous network on Au electrode surface to enable higher adsorption of target miRNA for CC interrogation of miRNA in presence of a redox molecule-ruthenium hexaammine (III) chloride ([Ru(NH3)6]3+, RuHex). Magnetically isolated and purified target miR-9-2 is adsorbed onto the MPGE via Au-RNA affinity interaction through the porous 3D network of the gel followed by the CC detection. The enhanced miRNA adsorption and electrocatalytic activity of MPGE/gel provide attomolar (50 aM) level of detection of miRNA with a dynamic range from 100pM to 10 aM. The good reproducibility (% RSD ≤ 5%, for n = 3) and high specificity of the developed biosensor demonstrates its excellent translational potential toward developing precisely controlled sensing devices for current clinical needs.

Original languageEnglish
Pages (from-to)198-207
Number of pages10
JournalBulletin of the Chemical Society of Japan
Volume95
Issue number1
DOIs
Publication statusPublished - 2022

Keywords

  • Gold electrode
  • Mesoporous metals
  • MicroRNA sensor

ASJC Scopus subject areas

  • Chemistry(all)

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