β-lactam resistance and β-lactamase isoforms of moraxella catarrhalis isolates in Taiwan

Chang Phone Fung, Sai Cheong Lee, Peter Yuk Fong Liu, Tsrang Neng Jang, Fu Der Wong, Benjamin I. Kuo, Cheng Yi Liu, Yung Chin Liu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Moraxella catarrhalis is an important pathogen in both upper and lower respiratory tract infections. More than 90% of isolates worldwide produce β- lactamase. The β-lactamases produced by M. catarrhalis can be differentiated by isoelectric focusing (IEF) into BRO-1 and BRO-2 patterns. In this study, we investigated the prevalence of various β-lactamase isoforms in clinical isolates of M. catarrhalis in Taiwan, as well as the relationships of these isoforms with antibiotic resistance. A total of 271 clinical isolates of M. catarrhalis were collected from 12 large medical laboratories in Taiwan from 1 August 1993 to 31 July 1995. The overall prevalence of β-lactamase production was 98.2% (266 of 271 isolates). Analytical IEF revealed BRO-1 was the most common β-lactamase pattern among the isolates (238 isolates, 88%); BRO-2 was the only other pattern found, with 32 (12%) isolates. The geometric mean minimum inhibitory concentration of ampicillin for BRO-1 producers was 63-fold higher than that for β-lactamase-negative isolates, and 6.5-fold higher than that for BRO-2 producers. β-Lactam antibiotics, such as amoxicillin + clavulanate and the cephalosporins, tested were very active against this species, regardless of whether the isolate produced β- lactamase or not. In conclusion, β-lactamase is common among clinical isolates of M. catarrhalis in Taiwan, with BRO-1 being the most common isoform. However, because most isolates tested were still sensitive to amoxicillin + clavulanate and cephalosporins, these agents appear to be reliable alternatives to first-line therapy when M. catarrhalis is contributing to a clinical infection.

Original languageEnglish
Pages (from-to)453-457
Number of pages5
JournalJournal of the Formosan Medical Association = Taiwan yi zhi
Volume97
Issue number7
Publication statusPublished - Jul 1998
Externally publishedYes

Fingerprint

Moraxella (Branhamella) catarrhalis
Lactams
Taiwan
Protein Isoforms
Clavulanic Acid
Amoxicillin
Isoelectric Focusing
Cephalosporins
Microbial Sensitivity Tests
Ampicillin
Microbial Drug Resistance
Respiratory Tract Infections
Anti-Bacterial Agents
Infection

Keywords

  • β-lactamase
  • Antibiotic resistance
  • Moraxella catarrhalis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

β-lactam resistance and β-lactamase isoforms of moraxella catarrhalis isolates in Taiwan. / Fung, Chang Phone; Lee, Sai Cheong; Liu, Peter Yuk Fong; Jang, Tsrang Neng; Wong, Fu Der; Kuo, Benjamin I.; Liu, Cheng Yi; Liu, Yung Chin.

In: Journal of the Formosan Medical Association = Taiwan yi zhi, Vol. 97, No. 7, 07.1998, p. 453-457.

Research output: Contribution to journalArticle

Fung, CP, Lee, SC, Liu, PYF, Jang, TN, Wong, FD, Kuo, BI, Liu, CY & Liu, YC 1998, 'β-lactam resistance and β-lactamase isoforms of moraxella catarrhalis isolates in Taiwan', Journal of the Formosan Medical Association = Taiwan yi zhi, vol. 97, no. 7, pp. 453-457.
Fung, Chang Phone ; Lee, Sai Cheong ; Liu, Peter Yuk Fong ; Jang, Tsrang Neng ; Wong, Fu Der ; Kuo, Benjamin I. ; Liu, Cheng Yi ; Liu, Yung Chin. / β-lactam resistance and β-lactamase isoforms of moraxella catarrhalis isolates in Taiwan. In: Journal of the Formosan Medical Association = Taiwan yi zhi. 1998 ; Vol. 97, No. 7. pp. 453-457.
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abstract = "Moraxella catarrhalis is an important pathogen in both upper and lower respiratory tract infections. More than 90{\%} of isolates worldwide produce β- lactamase. The β-lactamases produced by M. catarrhalis can be differentiated by isoelectric focusing (IEF) into BRO-1 and BRO-2 patterns. In this study, we investigated the prevalence of various β-lactamase isoforms in clinical isolates of M. catarrhalis in Taiwan, as well as the relationships of these isoforms with antibiotic resistance. A total of 271 clinical isolates of M. catarrhalis were collected from 12 large medical laboratories in Taiwan from 1 August 1993 to 31 July 1995. The overall prevalence of β-lactamase production was 98.2{\%} (266 of 271 isolates). Analytical IEF revealed BRO-1 was the most common β-lactamase pattern among the isolates (238 isolates, 88{\%}); BRO-2 was the only other pattern found, with 32 (12{\%}) isolates. The geometric mean minimum inhibitory concentration of ampicillin for BRO-1 producers was 63-fold higher than that for β-lactamase-negative isolates, and 6.5-fold higher than that for BRO-2 producers. β-Lactam antibiotics, such as amoxicillin + clavulanate and the cephalosporins, tested were very active against this species, regardless of whether the isolate produced β- lactamase or not. In conclusion, β-lactamase is common among clinical isolates of M. catarrhalis in Taiwan, with BRO-1 being the most common isoform. However, because most isolates tested were still sensitive to amoxicillin + clavulanate and cephalosporins, these agents appear to be reliable alternatives to first-line therapy when M. catarrhalis is contributing to a clinical infection.",
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