β-Endorphin

Deletion of a single amino acid residue abolishes immunoreactivity but retains opiate potency

Choh Hao Li, Wen Chang Chang, Donald Yamashiro, Liang Fu Tseng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Two synthetic analogs of camel β-endorphin, one with omission of Leu-14 and the other with omission of Asn-20, have been assayed for immunoreactivity by radioimmunoassay, opiate activity in the guinea pig ileum preparation and analgesic potency in mice. It was found that the omission analogs had no immunoreactivity, but retained significant biological activities. As far as we are aware, this is the first instance in which deletion of a single amino acid residue in a biologically active peptide abolished immunoreactivity.

Original languageEnglish
Pages (from-to)693-697
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume87
Issue number3
DOIs
Publication statusPublished - Apr 13 1979
Externally publishedYes

Fingerprint

Opiate Alkaloids
Endorphins
Camelus
Bioactivity
Ileum
Radioimmunoassay
Analgesics
Guinea Pigs
Amino Acids
Peptides

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

β-Endorphin : Deletion of a single amino acid residue abolishes immunoreactivity but retains opiate potency. / Li, Choh Hao; Chang, Wen Chang; Yamashiro, Donald; Tseng, Liang Fu.

In: Biochemical and Biophysical Research Communications, Vol. 87, No. 3, 13.04.1979, p. 693-697.

Research output: Contribution to journalArticle

@article{0ca932c16492403181eddd831123a3bc,
title = "β-Endorphin: Deletion of a single amino acid residue abolishes immunoreactivity but retains opiate potency",
abstract = "Two synthetic analogs of camel β-endorphin, one with omission of Leu-14 and the other with omission of Asn-20, have been assayed for immunoreactivity by radioimmunoassay, opiate activity in the guinea pig ileum preparation and analgesic potency in mice. It was found that the omission analogs had no immunoreactivity, but retained significant biological activities. As far as we are aware, this is the first instance in which deletion of a single amino acid residue in a biologically active peptide abolished immunoreactivity.",
author = "Li, {Choh Hao} and Chang, {Wen Chang} and Donald Yamashiro and Tseng, {Liang Fu}",
year = "1979",
month = "4",
day = "13",
doi = "10.1016/0006-291X(79)92014-X",
language = "English",
volume = "87",
pages = "693--697",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier B.V.",
number = "3",

}

TY - JOUR

T1 - β-Endorphin

T2 - Deletion of a single amino acid residue abolishes immunoreactivity but retains opiate potency

AU - Li, Choh Hao

AU - Chang, Wen Chang

AU - Yamashiro, Donald

AU - Tseng, Liang Fu

PY - 1979/4/13

Y1 - 1979/4/13

N2 - Two synthetic analogs of camel β-endorphin, one with omission of Leu-14 and the other with omission of Asn-20, have been assayed for immunoreactivity by radioimmunoassay, opiate activity in the guinea pig ileum preparation and analgesic potency in mice. It was found that the omission analogs had no immunoreactivity, but retained significant biological activities. As far as we are aware, this is the first instance in which deletion of a single amino acid residue in a biologically active peptide abolished immunoreactivity.

AB - Two synthetic analogs of camel β-endorphin, one with omission of Leu-14 and the other with omission of Asn-20, have been assayed for immunoreactivity by radioimmunoassay, opiate activity in the guinea pig ileum preparation and analgesic potency in mice. It was found that the omission analogs had no immunoreactivity, but retained significant biological activities. As far as we are aware, this is the first instance in which deletion of a single amino acid residue in a biologically active peptide abolished immunoreactivity.

UR - http://www.scopus.com/inward/record.url?scp=0018760353&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018760353&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(79)92014-X

DO - 10.1016/0006-291X(79)92014-X

M3 - Article

VL - 87

SP - 693

EP - 697

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -