β-Catenin overexpression causes an increase in inflammatory cytokines and NF-kB activation in cardiomyocytes

J. C. Lin, R. L. Chang, Y. F. Chen, J. J. Yang, R. Baskaran, L. C. Chung, R. J. Chen, C. H. Day, V. Vijaya Padma, C. Y. Huang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

β-Catenin has been implicated in various developmental and physiological processes. Defective Wnt signaling can result in different cardiac and vascular abnormalities and is activated under pathological conditions such as inflammation and obesity. In this study, roles of β-catenin in inflammation in cardiomyocytes were investigated. 10 samples from hearts of patients with acute infarction and 10 from normal ones were collected in order to access roles of β-catenin in cardiomyocytes. H9c2 cardiomyoblasts and primary neonatal rat cardiomyocytes were transfected with porcine cytomegalovirus (pCMV)-β-catenin plasmid in order to overexpress β-catenin. Protein level of β-catenin protein was increased in human acute infarction tissues compared to ones from normal patients. The transcription factor had increased nuclear localization in cardiomyocytes of the Wistar rats with cardiac hypertension. Furthermore, expression of fibrosis protein markers increased. Protein expression of β-catenin was increased in human acute infarction inflammatory heart tissues and in hearts of inflammatory obesity rats. After pCMV-β-catenin plasmid was transfected in a dose-dependent manner, inflammation protein markers, TNF-a and IL-8, were upregulated in hypertensive neonatal rat cardiomyocytes and H9c2 cardiomyoblasts. In addition, overexpression of β-catenin induced activation and nuclear localization of NF-kB. Therefore, β-catenin is a potential molecular target for treatment of inflammation and fibrosis in cardiomyocytes.

Original languageEnglish
Pages (from-to)17-22
Number of pages6
JournalCellular and Molecular Biology
Volume63
Issue number1
DOIs
Publication statusPublished - 2017

Fingerprint

Catenins
NF-kappa B
Cardiac Myocytes
Chemical activation
Cytokines
Rats
Infarction
Inflammation
Cytomegalovirus
Proteins
Plasmids
Fibrosis
Swine
Obesity
Tissue
Physiological Phenomena
Interleukin-8
Blood Vessels
Wistar Rats
Transcription Factors

Keywords

  • Acute infarction
  • H9c2 cardiomyocytes
  • Inflammation
  • NF-κB
  • β-catenin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Lin, J. C., Chang, R. L., Chen, Y. F., Yang, J. J., Baskaran, R., Chung, L. C., ... Huang, C. Y. (2017). β-Catenin overexpression causes an increase in inflammatory cytokines and NF-kB activation in cardiomyocytes. Cellular and Molecular Biology, 63(1), 17-22. https://doi.org/10.14715/cmb/2017.63.1.4

β-Catenin overexpression causes an increase in inflammatory cytokines and NF-kB activation in cardiomyocytes. / Lin, J. C.; Chang, R. L.; Chen, Y. F.; Yang, J. J.; Baskaran, R.; Chung, L. C.; Chen, R. J.; Day, C. H.; Vijaya Padma, V.; Huang, C. Y.

In: Cellular and Molecular Biology, Vol. 63, No. 1, 2017, p. 17-22.

Research output: Contribution to journalArticle

Lin, JC, Chang, RL, Chen, YF, Yang, JJ, Baskaran, R, Chung, LC, Chen, RJ, Day, CH, Vijaya Padma, V & Huang, CY 2017, 'β-Catenin overexpression causes an increase in inflammatory cytokines and NF-kB activation in cardiomyocytes', Cellular and Molecular Biology, vol. 63, no. 1, pp. 17-22. https://doi.org/10.14715/cmb/2017.63.1.4
Lin, J. C. ; Chang, R. L. ; Chen, Y. F. ; Yang, J. J. ; Baskaran, R. ; Chung, L. C. ; Chen, R. J. ; Day, C. H. ; Vijaya Padma, V. ; Huang, C. Y. / β-Catenin overexpression causes an increase in inflammatory cytokines and NF-kB activation in cardiomyocytes. In: Cellular and Molecular Biology. 2017 ; Vol. 63, No. 1. pp. 17-22.
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