β-Carotene and canthaxanthin alter the pro-oxidation and antioxidation balance in rats fed a high-cholesterol and high-fat diet

Chun Kuang Shih, Jui Hung Chang, Shwu Huey Yang, Tsui Wei Chou, Hsing-Hsien Cheng

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

This study investigated the effects of β-carotene and canthaxanthin on lipid peroxidation and antioxidative enzyme activities in rats fed a high-cholesterol, high-fat diet. Wistar rats were divided into six groups. Negative control group (group NC) received a high-fat (150 g/kg) diet; cholesterol control group (group CC) received a high-cholesterol (10 g/kg), high-fat diet. The other four groups were fed a high-cholesterol, high-fat diet supplemented with crystal β-carotene (group BC), β-carotene beadlet (group BB), canthaxanthin beadlet (group CX) or α-tocopherol (group AT). Blood and livers were collected for analysis after 6 weeks of feeding. Group BB had significantly lower hepatic thiobarbituric acid reactive substance (TBARS) and conjugated diene concentrations, whereas group CX had a significantly lower plasma TBARS concentration than did group CC. In erythrocytes, glutathione peroxidase activities were significantly greater in groups BC, BB and CX than in group CC. Moreover, compared with group CC, catalase activities were significantly greater in groups BB and CX, and superoxide dismutase (SOD) activity was significantly greater in group BB. In livers, SOD activities were significantly greater in groups BC, BB and CX, and glutathione reductase activities were significantly greater in groups BB and CX than in group CC. Compared with group CC, hepatic retinol and α-tocopherol concentrations were significantly greater in groups BC, BB and CX, whereas plasma and hepatic cholesterol concentrations were significantly lower in group BC. These findings suggest that β-carotene and canthaxanthin altered the pro-oxidation and antioxidation balance and suppressed cholesterol-induced oxidative stress via modulation of antioxidant system and cholesterol metabolism.

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalBritish Journal of Nutrition
Volume99
Issue number1
DOIs
Publication statusPublished - Jan 2008

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Canthaxanthin
High Fat Diet
Carotenoids
Cholesterol
Liver
Tocopherols
Thiobarbituric Acid Reactive Substances
Superoxide Dismutase
Control Groups
Glutathione Reductase
Glutathione Peroxidase
Vitamin A
Catalase
Lipid Peroxidation
Wistar Rats
Oxidative Stress
Antioxidants
Erythrocytes
Fats
Diet

Keywords

  • β-Carotene
  • Antioxidation
  • Canthaxanthin
  • Cholesterol
  • Pro-oxidation

ASJC Scopus subject areas

  • Medicine (miscellaneous)

Cite this

β-Carotene and canthaxanthin alter the pro-oxidation and antioxidation balance in rats fed a high-cholesterol and high-fat diet. / Shih, Chun Kuang; Chang, Jui Hung; Yang, Shwu Huey; Chou, Tsui Wei; Cheng, Hsing-Hsien.

In: British Journal of Nutrition, Vol. 99, No. 1, 01.2008, p. 59-66.

Research output: Contribution to journalArticle

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