Abstract

Vitamin E (Vit. E) is considered an essential dietary nutrient for humans and animals. An enormous body of evidence indicates the biological and protective effects of Vit. E consumption. Tocopherol-associated protein (TAP) is a major tocopherolbinding protein affecting Vit. E stimulation and downstream signaling transduction. However, how Vit. E utilizes TAP as an anti-cancer mechanism remains unclear. Microarray analysis of signature gene profiles in breast cancer cells treated with α-tocopheryl succinate (α-TOS, a Vit. E isoform) resulted in cell cycle arrest and anticancer activity in breast cancer cells. Pterostilbene (PS), a natural dietary antioxidant found in blueberries, in combination with α-TOS synergistically maximized breast cancer cell growth inhibition by disrupting signal transduction, transcription factors and cell cycle proteins. In a xenograft mouse model, PS treatment with Vit. E inhibited breast tumor growth and cell invasion, which were evaluated using our recently developed circulating tumor cell (CTC) detection assay. Because dietary Vit. E and PS supplementation contributed to preventative and therapeutic effects in vitro and in vivo, this combination may benefit breast cancer therapy in the clinic.

Original languageEnglish
Pages (from-to)4593-4606
Number of pages14
JournalOncotarget
Volume9
Issue number4
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

alpha-Tocopherol
Vitamin E
Human Activities
Breast Neoplasms
Neoplasms
Tocopherols
Blueberry Plants
Circulating Neoplastic Cells
Cell Cycle Proteins
Proteins
Succinic Acid
Therapeutic Uses
Microarray Analysis
Growth
Cell Cycle Checkpoints
pterostilbene
In Vitro Techniques
Heterografts
Signal Transduction
Protein Isoforms

Keywords

  • A-tocopheryl succinate
  • Breast cancer
  • Pterostilbene
  • Tocopherol-associated protein
  • Vitamin E

ASJC Scopus subject areas

  • Oncology

Cite this

α-Tocopherol succinate enhances pterostilbene anti-tumor activity in human breast cancer cells in vivo and in vitro. / Tam, Ka Wai; Ho, Chi Tang; Tu, Shih Hsin; Lee, Wen Jui; Huang, Ching Shui; Chen, Ching Shyang; Wu, Chih Hsiung; Lee, Chia Hwa; Ho, Yuan Soon.

In: Oncotarget, Vol. 9, No. 4, 01.01.2018, p. 4593-4606.

Research output: Contribution to journalArticle

Tam, Ka Wai ; Ho, Chi Tang ; Tu, Shih Hsin ; Lee, Wen Jui ; Huang, Ching Shui ; Chen, Ching Shyang ; Wu, Chih Hsiung ; Lee, Chia Hwa ; Ho, Yuan Soon. / α-Tocopherol succinate enhances pterostilbene anti-tumor activity in human breast cancer cells in vivo and in vitro. In: Oncotarget. 2018 ; Vol. 9, No. 4. pp. 4593-4606.
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