Probing Real-Time Activation of Nf-Kappa B Signaling Circuits by Low Level Laser Therapy in Single Living Cells

Project: A - Government Institutionb - Ministry of Science and Technology

Project Details

Description

The purpose of this project is to investigate the activation of NF-kappa B signaling circuits by low level laser therapy (LLLT) in single living cells using fluorescence-based technology, together with our high-resolution optical tweezers system, fluorescence detection system, microfluidic system, accurate sample temperature control system, micro-scale surface enhanced Raman spectroscopy system, laser-guided direct writing cell bioprinting system and image-based autofocusing system. This seven in one system allows us to probe the generation of reactive oxygen species (ROS) and the real-time activation of NF-kappa B signaling circuits by LLLT in single living cells. This project will primarily focus on three specific aims, and we will outline them briefly: Aim 1: Construction of precise near-infrared laser system, together with the microfluidic cell culture platform and fluorescence microscopy for LLLT; Aim 2: Probing real-time fluorescent signals of both ROS and NF-kB using two-channel, simultaneous-imaging system, and characterizing the effects of LLLT dose on ROS formation and NF-kB activation, and Aim 3: Validate whether the dysfunction of mitochondria due to the presence of either a mitochondrial uncoupler, carbonyl cyanide m-chlorophenylhydrazone (CCCP) or knockdown of cytochrome c oxidase can be naturally restored via LLLT. We anticipate the proposed LLLT in single living cells is an effective treatment strategy and LLLT can naturally restore mitochondrial function.
StatusFinished
Effective start/end date8/1/187/1/19

Keywords

  • Low level light therapy
  • mitochondria
  • cytochrome c oxidase
  • reactive oxygen species