In previous studies, a patented high-yield and non-dialysis silk fibroin regeneration technology based on the shear-induced phase separation was established. The objective of this study is to develop photocrosslinkable methacrylated silk fibroin/Sacchachitosan (SF/SC) composites for bone tissue engineering applications via 3D printing technology. The degummed silk cocoon was first dissolved in a (CaCh • 2H2O/C2H5OH/H2O) solution, and then silk fibroin was harvested by a non-dialysis process. Various compositions of SF/SC were turned into photocrosslinkable by methacrylation according to Michael addition reaction. By incorporating methacrylated SF/SC with nano-hydroxyapatite (nano-HAp) or MC3T3 E1 cells individually then the bone scaffolds were prepared by 3D printing processes. A two-year project is proposed. In the first year, the nano-HAp and methacrylated SF/SC will be prepared and characterized by FT-IR, 1H-NMR, XRD, and rheometer for their structural, physical, and rheological properties. A SF/SC/nano-HAp bone bio-scaffold will be 3D printed based on the STL (Stereolithography) file built from Solid WorksTM software and assayed for its biocompatibility and mechanical properties. In the second year, the photocrosslink 3D-print operating parameters and cell viability on SF/SC/MC3T3 E1 cells based scaffold will be carried out. It will shed some light on the future development of high-end photocrosslinkable biomaterial for 3D printing technology.
|Effective start/end date||8/1/15 → 7/31/16|